2010 Fiscal Year Final Research Report
Molecular Genetic Analysis of the MAPK Signaling Pathway
| Project/Area Number |
19390024
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kinki University |
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Principal Investigator |
SUGIURA Reiko Kinki University, 薬学部, 教授 (90294206)
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| Co-Investigator(Kenkyū-buntansha) |
KITA Ayako 近畿大学, 薬学部, 助教 (00388498)
KAKEHI Kazuaki 近畿大学, 薬学部, 教授 (30101405)
MURAOKA Osamu 近畿大学, 薬学部, 教授 (20142599)
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| Project Period (FY) |
2007 – 2010
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| Keywords | MAPキナーゼ / 細胞内シグナル伝達 / ゲノム創薬 / ゲノム薬理 / 分裂酵母モデル生物 / RNA結合タンパク質 / 分子遺伝学 / タンパク質リン酸化 |
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| Research Abstract |
This project aims to identify regulators of MAPK signaling using fission yeast S. pombe as a model organism. We identified negative regulators of Pmk1 MAPK, such as ptc1+, ptc3+, encoding PP2Cs, and ecm33+, encoding a cell surface protein. We also identified targets of Pmk1 including the Atf1 transcription factor and the Nrd1 RNA-binding protein. We also demonstrated feedback mechanisms involving these regulators of Pmk1 MAPK and identified several compounds with potent MAPK inhibitory action.
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[Journal Article] The Cell Surface Protein Gene ecm33^+ Is a Target of the Two Transcription Factors Atf1 and Mbx1 and Negatively Regulates Pmk1 MAPK Cell Integrity Signaling in Fission Yeast.2010
Author(s)
Hirohumi Takada, Aiko Nishida, Mitsuhiro Domae, Ayako Kita, Yuki Yamano, Atsushi Uchida, Shunji Ishiwata,Yue Fang , Xin Zhou, Takashi Masuko, Mitsuhiro Kinoshita, Kazuaki Kakehi, Reiko Sugiura
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Journal Title
Mol.Biol.Cell. 21(4)
Pages: 674-85
Peer Reviewed
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[Journal Article] Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeast. The Role of the RNA-Binding Protein Nrd1 and Pmk1 MAPK in the Regulation of Myosin mRNA Stability in Fission Yeast2009
Author(s)
Yan Ma, Mai Takeuchi, Reiko Sugiura, Susie O. Sio, Takayoshi Kuno Ryosuke Satoh, Takahiro Morita, Hirofumi Takada, Ayako Kita, Shunji Ishiwata, Akira Doi, Kanako Hagihara, Atsushi Taga, Yasuhiro Matsumura, Hideki Tohda, Reiko Sugiura
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Journal Title
Genes Cells Mol.Biol.Cell. 14,20(9)
Pages: 1015-1028,2473-2485
Peer Reviewed
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[Journal Article] Atf1 Is a Target of the MAP Kinase Pmk1 and Regulates Cell Integrity in Fission Yeast.2007
Author(s)
Hirofumi Takada, Masayuki Nishimura, Yuta Asayama, Yoshiaki Mannse, Shunji Ishiwata, Ayako Kita, Akira Doi, Aiko Nishida, Naoyuki Kai, Sayako Moriuchi, Hideki Tohda, Yuko Giga-Hama, Takayoshi Kuno, Reiko Sugiura
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Journal Title
Mol.Biol.Cell 18(12)
Pages: 4794-4802
Peer Reviewed
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