Therapeutic principles on protein misfolding diseases

2009 Fiscal Year Final Research Report

• Project/Area Number: 19390045
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Kumamoto University
• Principal Investigator: KAI Hirofumi Kumamoto University, 大学院・生命科学研究部, 教授 (30194658)
• Co-Investigator(Renkei-kenkyūsha): SHUTO Ysuyoshi 熊本大学, 大学院・生命科学研究部, 講師 (80333524) ; SUICO MARY Ann Soten 熊本大学, 大学院・生命科学研究部, 助教 (20363525)
• Project Period (FY): 2007 – 2009
• Keywords: 薬理学 / タンパク質 / 遺伝病 / cystic fibrosis / FAP / CFTR / transthyretin

Research Abstract

Drug development for cystic fibrosis and familial amyloid polyneuropathy is still in progress. We found out the intracellular protein quality control mechanisms of ΔF508 CFTR, a major mutant of cystic fibrosis, and of transthyretin variants, resulting in proposal of new concept of drug development for familial amyloid polyneuropathy. In addition, we found that the endoplasmic reticulum-associated degradation of transthyretin variants is negatively regulated by BiP in mammalian cells.

Research Products

• [Journal Article] Role of the glutamic acid 54 residue in transthyretin stability and thyroxine binding (2010)
  • Author(s): Miyata M, Sato T, Mizuguchi M, Nakamura T, Ikemizu S, Nabeshima Y, Susuki S, Suwa Y, Morioka H, Ando Y, Suico MA, Shuto T, Koga T, Yamagata Y, Kai H
  • Journal Title: Biochemistry 49 Pages: 114-23
  • Peer Reviewed
• [Journal Article] Heat shock treatment with mild electrical stimulation safely reduced inflammatory markers in healthy male subjects (2010)
  • Author(s): Kondo, T., Sasaki, K., Adachi, H., Nakayama, Y., Hatemura, M., Matsuyama, R., Tsuruzoe, K., Furukawa, N., Motoshima, H., Morino, S., Yamashita Y., Miyamura N., Kai H., Araki E
  • Journal Title: Obesity Research, Clinical Practice 4 Pages: e101-e109
  • Peer Reviewed
• [Journal Article] TLR3 induction by anticancer drugs potentiates poly I:C-induced tumor cell apoptosis (2010)
  • Author(s): Manabu Taura, R. Fukuda, Mary Ann Suico, Ayaka Eguma, Tomoaki Koga, Tsuyoshi Shuto, Takashi Sato, Saori Koga Morino, Hirofumi Kai
  • Journal Title: Cancer Sci (in press)
  • Peer Reviewed
• [Journal Article] Modified Mild Heat Shock Modality Attenuates Hepatic Ischemia/Reperfusion Injury (2010)
  • Author(s): Mariko Oba, Mary Ann Suico, Saori Morino, Shuichiro Yano, Takashi Matsuno, Tomoaki Koga, Takashi Sato, Tsuyoshi Shuto, Hirofumi Kai
  • Journal Title: J. Surg. Res (in press)
  • Peer Reviewed
• [Journal Article] The endoplasmic reticulum-associated degradation of transthyretin variants is negatively regulated by BiP in mammalian cells (2009)
  • Author(s): Susuki S, Sato T, Miyata M, Momohara M, Suico MA, Shuto T, Ando Y, Kai H
  • Journal Title: J Biol Chem 284 Pages: 8312-8321
  • Peer Reviewed
• [Journal Article] Calreticulin positively regulates the expression and function of epithelial sodium channel (2009)
  • Author(s): Takuya Sugahara, Tomoaki Koga, Keiko Ueno-Shuto, Tsuyoshi Shuto, Eriko Watanabe, Maekawa A., Kitamura K., Tomita K., Ai Mizuno, Takashi Sato, Mary Ann Suico, Hirofumi Kai
  • Journal Title: Exp. Cell Res 315 Pages: 3294-300
  • Peer Reviewed
• [Journal Article] A novel combination of mild electrical stimulation and hyperthermia: general concepts and applications (review) (2009)
  • Author(s): Hirofumi Kai, Mary Ann Suico, Saori Morino, Tatsuya Kondo, Mariko Oba, Mikiko Noguchi, Tsuyoshi Shuto, Eiichi Araki
  • Journal Title: Int. J. Hyperthermia 25 Pages: 655-660
  • Peer Reviewed
• [Journal Article] The expression of antimicrobial peptide lysozyme is increased by treatment with silver nanoparticle (Atomyball SR) in mammalian epithelial cells (2009)
  • Author(s): Mary Ann Suico, Atsushi Tanaka, Tsuyoshi Shuto, Hirofumi Kai
  • Journal Title: J. Health Sci 55 Pages: 456-462
  • Peer Reviewed
• [Journal Article] Phosphatidic acid metabolism regulate the intracellular trafficking and retrotranslocation of cystic fibrosis transmembrane conductance regulator. Biochem (2008)
  • Author(s): Yasuaki Hashimoto, Tsukasa Okiyoneda, Kazutsune Harada, Keiko Ueno, Takuya Sugahara, Atsushi Yamashita, Tsuyoshi Shuto, Mary Ann Suico, Hirofumi Kai
  • Journal Title: Biochim. Acta (Mol. Cell Res. ) 1783 Pages: 153-162
  • Peer Reviewed
• [Journal Article] Multi-elemental analysis of serum and amyloid fibrils in familial amyloid polyneuropathy patients (2008)
  • Author(s): Susuki S., Ando Y., Sato T., Nishiyama M., Miyata M., Suico MA., Shuto T., Kai H
  • Journal Title: Amyloid (The Journal of Protein Folding Disorders) 15 Pages: 108-116
  • Peer Reviewed
• [Journal Article] Interferon-gamma down-regulated Hsp27 and enhances hyperthermia-induced tumor cell death in vitro and tumor suppression in vivo (2008)
  • Author(s): Oba M., Yano S., Shuto T., Suico M.A., Eguma A., Kai H
  • Journal Title: Int. J. Oncol 32 Pages: 1317-1324
  • Peer Reviewed
• [Journal Article] Role of calnexin in the ER quality control and productive folding of CFTR; differential effect of calnexin knockout on wild-type and DF508 CFTR (2008)
  • Author(s): Okiyoneda T., Niibori A., Harada K., Kohno T., Michalak M., Duszyk M., Wada I., Ikawa M., Shuto T., Suico M. A, Kai H
  • Journal Title: Biochem. Biochim. Acta (Mol. Cell Res. ) 1783 Pages: 1585-94
  • Peer Reviewed
• [Journal Article] DNA demethylation-dependent enhancement of toll-like receptor-2 gene expression in cystic fibrosis epithelial cells involves SP1-activated transcription (2008)
  • Author(s): Takashi Furuta, Tsuyoshi Shuto, Shogo Shimasaki, Yuko Ohira, Mary Ann Suico, Dieter C Gruenert, Hirofumi Kai
  • Journal Title: BMC Mol Biol 9 Pages: 39
  • Peer Reviewed
• [Journal Article] p53 regulates TLR3 expression and function in human epithelial cell lines (2008)
  • Author(s): Manabu Taura, Ayaka Eguma, Mary Ann Suico, Tsuyoshi Shuto, Tomoaki Koga, Kensei Komatsu, Takefumi Komune, Takashi Sato, Hideyuki Saya, Jian-Dong Li, Hirofumi Kai
  • Journal Title: Mol. Cell. Biol 28 Pages: 6557-67
  • Peer Reviewed
• [Journal Article] Mild Electrical Stimulation Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degredation (2008)
  • Author(s): Saori Morino, Mary Ann Suico, Tatsuya Kondo, Erika Sekimoto, Shuichiro Yano. Tomoko Matsuda, Takashi Matsuno, Tsuyoshi Shuto, Eiichi Araki, Hirohumi Kai
  • Journal Title: J Pharmacol Sci 108 Pages: 222-226
  • Peer Reviewed
• [Journal Article] Mild Electrical Stimulation with heat shock ameliorates insulin resistance via enhanced insulin signaling. Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degradation (2008)
  • Author(s): Saori Morino, Tatsuya Kondo, Kazunari Sasaki, Hironori Adachi, Mary Ann Suico, Erika Sekimoto, Tomoko Matsuda, Tsuyoshi Shuto, Eiichi Araki, Hirofumi Kai
  • Journal Title: PLoS ONE 3(12) Pages: e4068
  • Peer Reviewed
• [Journal Article] Curucumin enhances cystic fibrosis transmembrane regulator expression by down-regulating calreticulin, Biochem (2007)
  • Author(s): Kazutsune Harada, Tsukasa Okiyoneda, Yasuaki Hashimoto, Kimiko Oyokawa, Kimitoshi Nakamura, Mary Ann Suico, Tsuyoshi Shuto, Hirofumi Kai
  • Journal Title: Biophys. Res. Commun 353 Pages: 351-356
  • Peer Reviewed
• [Journal Article] Increased responsiveness to TLR2 and TLR4 ligands during dimethylsulfoxideinduced neutrophil-like differentiation of HL-60 myeloid leukemia cells (2007)
  • Author(s): Tsuyoshi Shuto, Takashi Furuta, Judy Cheung, Dieter C. Gruenert, Yuko Ohira, Shogo Shimasaki, Mary Ann Suico, Keizo Sato, Hirofumi Kai
  • Journal Title: Leuk Res 31 Pages: 1721-8
  • Peer Reviewed
• [Journal Article] Endoplasmic reticulum quality control regulates the fate of transthyretin variants in the cell
  • Author(s): Takashi Sato, Seiko Susuki, Mary Ann Suico, Masanori Miyata, Yukio Ando, Mineyuki Mizuguchi, Makoto Takeuchi, Mizuki Dobashi, Tsuyoshi Shuto, Hirofumi Kai
  • Journal Title: EMBO J 26 Pages: 2501-2512
  • Peer Reviewed
• [Presentation] Combination of heat shock and mild electrical stimulation improves insulin resistance and visceral fat accumulation (2009)
  • Author(s): S. Morino
  • Organizer: The 4th International Congress on Stress Responses in Biology and Medicine
  • Place of Presentation: Gateaux Kingdom Sapporo Hotel, Spa Resort, Sapporo, Japan
  • Year and Date: 20091006-20091009
• [Presentation] Endoplasmic reticulum stress increases the expression of toll-like receptor-2 in epithelial cells (2009)
  • Author(s): S. Shimasaki
  • Organizer: Cell Stress Society International 2009
  • Place of Presentation: Gateaux Kingdom Sapporo Hotel, Spa Resort, Sapporo, Japan
  • Year and Date: 20091006-20091009
• [Presentation] The endoplasmic reticulum-associated degradation of transthyretin variants is negatively regulated by BiP (2009)
  • Author(s): T. Sato
  • Organizer: Cell Stress Society International 2009
  • Place of Presentation: Gateaux Kingdom Sapporo Hotel, Spa Resort, Sapporo, Japan
  • Year and Date: 20091006-20091009
• [Presentation] The endoplasmic reticulum-associated degradation of transthyretin variants is negatively regulated by BiP in mammalian cells (2009)
  • Author(s): T. Sato
  • Organizer: FASEB SUMMER RESEARCH CONFERENCES
  • Place of Presentation: Vermont Academy, Vermont, USA
  • Year and Date: 20090607-20090612
• [Presentation] The role of Glu54 in transthyretin for protein stability, thyroxine binding and secretion from the cells (2009)
  • Author(s): M. Miyata
  • Organizer: FASEB SUMMER RESEARCH CONFERENCES
  • Place of Presentation: Vermont Academy, Vermont, USA
  • Year and Date: 20090607-20090612
• [Presentation] p53 Regulates TLR3 Expression and Function in Human Epthelial Cell Lines (2008)
  • Author(s): A. Eguma
  • Organizer: The American Society for Cell Biology 48th Annual Meeting
  • Place of Presentation: Moscon convention center, San Francisco, USA
  • Year and Date: 20081207-20081213
• [Presentation] Curcumin Inhibits Peptidoglycan-Mediated Inflammatory Response in Human Cystic Fibrosis Bronchial Epithelial Cells through Inhibition of TLR2 Gene (2008)
  • Author(s): Y. Ohira
  • Organizer: The 22nd Annual North American Cystic Fibrosis Conference
  • Place of Presentation: Orland, Florida, USA
  • Year and Date: 20081023-20081025
• [Presentation] ER RETENTION OF CFTR IS CRUCIAL FOR SP1-DEPENDENT UP-REGULATION OF TLR2 EXPRESSION INΔF508-CFTR-EXPRESSING CF BRONCHIAL EPITHELIAL CELLS (2008)
  • Author(s): S. Shimasaki
  • Organizer: The 22nd Annual North American Cystic Fibrosis Conference
  • Place of Presentation: Orland, Florida, USA
  • Year and Date: 20081023-20081025
• [Presentation] The endoplasmic reticulm retention and degradation of transthyretin are regulated by the molecular chaperone BiP in mammalian cells (2008)
  • Author(s): S. Susuki
  • Organizer: VIIth International Symposium on Familial Amyloid Polyneuropathy and Ist International Workshop on Hereditary Amyloidosis
  • Place of Presentation: King's College London, London, UK
  • Year and Date: 20080902-20080905
• [Presentation] The role of coat protein complex II machinery on the secretion, endoplasmic reticulum retention and endoplasmic reticulum-associated degradation of transthyretin (2008)
  • Author(s): T. Sato
  • Organizer: VIIth International Symposium on Familial Amyloid Polyneuropathy and Ist International Workshop on Hereditary Amyloidosis
  • Place of Presentation: King's College London, London, UK
  • Year and Date: 20080902-20080905
• [Presentation] Glu54 mutations in transthyretin affect thyroxine binding and tetramer stability, resulting in aggressive familial amyloid polyneuropathy (2008)
  • Author(s): M. Miyata
  • Organizer: VIIth International Symposium on Familial Amyloid Polyneuropathy and Ist International Workshop on Hereditary Amyloidosis
  • Place of Presentation: King's College London, London, UK
  • Year and Date: 20080902-20080905
• [Presentation] Phosphatidic Acid Metabolism Regulates the Intracellular Trafficking of CFTR (2007)
  • Author(s): S. Mizunoe
  • Organizer: The American Society for Cell Biology 47th Annual Meeting
  • Place of Presentation: Washington Convention Center, Washington, DC., USA
  • Year and Date: 20071201-20071205
• [Presentation] The Expression of Myelid-1-like Factor (MEF/Elf4), an ETS Transcription Factor, Is Modulated by p53 (2007)
  • Author(s): M. Taura
  • Organizer: The American Society for Cell Biology 47th Annual Meeting
  • Place of Presentation: Washington Convention Center, Washington, DC., USA
  • Year and Date: 20071201-20071205
• [Presentation] Investigation of the Mechanism of Severe Familial Amyloidotic Polyneurophaty (2007)
  • Author(s): M. Miyata
  • Organizer: The American Society for Cell Biology 47th Annual Meeting
  • Place of Presentation: Washington Convention Center, Washington, DC., USA
  • Year and Date: 20071201-20071205
• [Presentation] Interferon-gamma Enhances the Induction of Cell Death by Heat-shock and Cisplatin through Hsp27 Down-Regulation (2007)
  • Author(s): M. Oba
  • Organizer: The American Society for Cell Biology 47th Annual Meeting
  • Place of Presentation: Washington Convention Center, Washington, DC., USA
  • Year and Date: 20071201-20071205
• [Remarks]
  • URL: http://www.molmed730.org/