2008 Fiscal Year Final Research Report
Mechanism and treatment for the disease caused by the impairment of PKC signaling
Project/Area Number |
19390064
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General pharmacology
|
Research Institution | Kobe University |
Principal Investigator |
SAITO Naoaki Kobe University, 自然科学系先端融合研究環バイオシグナル研究センター, 教授 (60178499)
|
Co-Investigator(Kenkyū-buntansha) |
SHIRAI Yasuhito 神戸大学, 自然科学系先端融合研究環バイオシグナル研究センター, 准教授 (60263399)
UEYAMA Takehiko 神戸大学, 自然科学系先端融合研究環バイオシグナル研究センター, 講師 (80346254)
|
Project Period (FY) |
2007 – 2008
|
Keywords | プロテインキナーゼC / 精髄小脳変性症 / カルシウム / 神経変性 / 遺伝子変異 |
Research Abstract |
脊髄小脳変性症14型の発症メカニズムとして、変異型PKCγには細胞膜に十分な時間存在する能力が欠如しTRPCチャネルをリン酸化することができないため細胞外からのCa^<2+> 流入を抑制する機構が働かないことを示した。この過剰なCa^<2+> 流入が神経細胞死が招くと推測された。また他の要因として初代培養プルキンエ細胞において変異型PKCγは凝集体の形成・樹状突起の伸展・シナプス形成異常を引き起こす事を示した。
|
-
[Journal Article] Kinase activity of the dgk gene product is involved 1 in the virulence of Streptococcus mutans Microbiology2009
Author(s)
Shibata, Y., van der Ploeg, JR., Kozuki, T., Shirai, Y., Saito, N., Kawada, M., Takeshita, T., and Yamashita, Y.
-
Journal Title
Peer Reviewed
-
[Journal Article] Mutant γPKC found in spinocerebellar ataxia type 14 induces aggregate-independent maldevelopment of dendrites in primary cultured Purkinje cells2009
Author(s)
Seki, T. ; Shimahara ; T., Yamamoto ; K., Abe ; N., Amano, T. ; Adachi ; N., Takahashi, H. ; Kashiwagi, K. ; Saito, N., Sakai, N.
-
Journal Title
Neurobiology of Disease 33
Pages: 260-273
Peer Reviewed
-
[Journal Article] Diacylglycerol kinase β accumulates on the perisynaptic site of medium spiny neurons in the striatum2008
Author(s)
Hozumi,Y., Fukaya, M., Adachi, N., Saito, N., Otani, K., Kondo, K., Watanabe, M., and Goto, K.
-
Journal Title
Eur. J. Neurosci 28
Pages: 2409-2422
Peer Reviewed
-
[Journal Article] Ca^<2+> oscillation induced by P2Y2 receptor activation and its regulation by a neuron-specific subtype of PKC (βPKC)2008
Author(s)
Ashida, N., Ueyama, T., Rikitake, K., Shirai, Y. Eto, M., Kondoh, T., Kohmura, E., and Saito, N.
-
Journal Title
Neurosci. Lett 446
Pages: 123-128
Peer Reviewed
-
[Journal Article] Metabotropic glutamate receptor subtype-1 is essential for in vivo growth of melanoma2008
Author(s)
Ohtani, Y., Harada, T., Funasaka, Y., Nakao, K., Takahara, C., Abdel-Daim, M., Sakai, N., Saito, N., Nishigori, C. and Aiba, A.
-
Journal Title
Oncogene 27
Pages: 7162-7170
Peer Reviewed
-
[Journal Article] Reactive oxygen species derived from NOX1/NADPH oxidase enhance inflammatory pain2008
Author(s)
Ibi, M., Matsuno, K., Shiba, D., Katsuyama, M., Iwata, K., Kakehi, T., Nakagawa, T., Sango, K., Shirai, Y., Yokoyama, T., Kaneko, S., Saito N.,and Yabe-Nishimura, C.
-
Journal Title
J. Neurosci 28
Pages: 9486-9494
Peer Reviewed
-
-
[Journal Article] Enzymological analysis of mutant protein kinase Cγ causing spinocerebellar ataxia type 14 and its dysfunction in Ca^<2+> homeostasis2008
Author(s)
Adachi, N., Kobayashi, T., Takahashi, H., Kawasaki, T., Shirai, Y., Ueyama, T., Matsuda, T., Seki, T., Sakai, N. and Saito N.
-
Journal Title
J. Biol. Chem 283
Pages: 19854-19863
Peer Reviewed
-
[Journal Article] Identification of SF-1/Ad4BP gene expression modulators by large scale gain and loss of function studies2008
Author(s)
Sakai, N., Terami, H., Suzuki, S., Haga, M.,Nomoto, K., Tsuchida, N., Morohashi, KI.,Saito, N., Asada, M., Hashimoto, M.,Harada, D., Asahara, H., Ishikawa, T.,Shimada, F., Sakurada, K.
-
Journal Title
J. Endocrinol 198
Pages: 489-497
Peer Reviewed
-
[Journal Article] Sequential Binding of Cytosolic Phox Complex to Phagosomes through Regulated Adaptor Proteins : Evaluation Using the Novel Monomeric Kusabira-Green System and Live Imaging of Phagocytosis2008
Author(s)
Ueyama, T., Kusakabe, T., Karasawa, S., Kawasaki, T., Shimizu, A., Son J-H, Shirai, Y., Leto, TL., Miyawaki, A., and Saito N.
-
Journal Title
J. Immunol 181
Pages: 629-40
Peer Reviewed
-
-
[Journal Article] Immunogold electron microscopic demonstration of distinct submembranous localization of the activated γPKC depending on the stimulation2008
Author(s)
Oyasu, M., Fujimiya, M., Kashiwagi, K., Ohmori, S., Imaeda, H. and Saito, N.
-
Journal Title
J. Histochem. Cytochem 56
Pages: 253-265
Peer Reviewed
-
[Journal Article] Synthesis Conformational Analysis, and Biological Evaluation of 1-Hexyl-indolactam-V10 as a Selective Activator for Novel Protein Kinase C Isozymes2008
Author(s)
Yanagita, RC, Nakagawa, Y., Yamanaka, N., Kashiwagi, K., Saito, N. and Irie, K.
-
Journal Title
J. Med. Chem 51
Pages: 46-56
Peer Reviewed
-
-
[Journal Article] Aggregate Formation of Mutant Protein Kinase C Gamma Found in Spinocerebellar Ataxia Type 14 Impairs Ubiquitin Proteasome System and Induces Endoplasmic Reticulum Stress Eur2007
Author(s)
Seki, T., Takahashi, T., Adachi, N., Shimahara, T., Abe, N., Saito, N. and Sakai N.
-
Journal Title
J. Neurosci 26
Pages: 3126-3140
Peer Reviewed
-
-
-
-
-
-
-
-
[Presentation] Sequential binding of cytosolic phox complex to phagosomes through regulated adaptor proteins : evaluation using the novel monomeric Kusabira-Green(mKG)system and liveimaging of phagocytosis2008
Author(s)
Ueyama,T., Kusakabe,T., Karasawa,S., Kawasaki,T., Shimizu,A., Son, Jeong-hyun., Leto,L.Thomas., Miyawaki,A., Saito,N.
Organizer
Gordon Research Conference
Place of Presentation
ボストン
Year and Date
20080601-07
-
-
-
[Presentation] Sequential targeting of cytosolic phox proteins to phagosomes through regulated adaptor proteins during Fc□R-mediated phagocytosis2008
Author(s)
Ueyama,T., Kusakabe,T., Karasawa,S., Kawasaki,T., Shimizu,A., Son,eong-hyun., Leto L.Thomas
Organizer
第81回日本薬理学会年会
Place of Presentation
横浜
Year and Date
2008-03-18
-
[Presentation] Enzymological properties of PKCγ mutants found in spinocerebellar ataxia 14(SCA14) and its involvement in neuronal death2007
Author(s)
Saito,N., Adachi,N., Takahashi,H., Kobayashi,T., Seki,T. Sakai,N.
Organizer
International symposium on cell signaling and gene regulation
Place of Presentation
Taiwan
Year and Date
20071118-19
-
-
-
-
-
-
-
-
-
-
-
-
-