2010 Fiscal Year Final Research Report
Development of the technology of nuclear reprogramming of human somatic cells to pluripotent stem cell
| Project/Area Number |
19390072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
TADA Takashi Kyoto University, 再生医科学研究所, 准教授 (30188247)
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| Project Period (FY) |
2007 – 2010
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| Keywords | ES細胞 / 体細胞 / 細胞融合 / 拒絶反応 / 再プログラム化 |
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| Research Abstract |
To develop technology of nuclear reprogramming of somatic cells into pluripotent stem cells, human somatic cells were cell-fused with human embryonic stem (ES) cells. We got the following results ; 1) human ES cells had potential ofreprogramming of human somatic cells into pluripotent stem cells, 2) selected chromosome (s) were eliminated from ES-somatic hybrid cells, and 3) karyotypically normal ES cells were generated by duplication of an eliminated chromosome. These developed techniques could be applied to induced pluripotent stem (iPS) cells.
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Research Products
(41 results)
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[Journal Article] Familiarity and prudence of the Japanese public with research into induced pluripotent stem cells, and their desire for its proper regulation.2010
Author(s)
Shineha, R., Kawakami, M., Kawakami, K., Nagata, M., Tada, T., Kato, K.
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Journal Title
Stem Cell Review 6
Pages: 1-7
Peer Reviewed
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[Journal Article] Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells.2009
Author(s)
Yamaguchi, S., Kurimoto, K., Yabuta, Y., Sasaki, H., Nakatsuji, N., Saitou, M., Tada, T.
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Journal Title
Development 136
Pages: 4011-4020
Peer Reviewed
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[Journal Article] Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells.2009
Author(s)
Nagata, S., Toyoda, M., Yamaguchi, S., Hirano, K., Makino, H., Nishino, K., Miyagawa, Y., Okita, H., Kiyokawa, N., Nakagawa, M., Yamanaka, S., Akutsu, H., Umezawa, A., Tada, T.
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Journal Title
Genes Cells 14
Pages: 1395-1404
Peer Reviewed
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[Journal Article] Rapid induction of large chromosomal deletions by a Cre/inverted loxP system in mouse ES Cell hybrids.2008
Author(s)
Otsuji, T., Matsumura, H., Suzuki, T., Nakatsuji, N., Tada, T., Tada, M.
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Journal Title
J Mol Biol 378
Pages: 328-336
Peer Reviewed
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[Journal Article] Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling.2008
Author(s)
Mise, N., Fuchikami, T., Sugimoto, M., Kobayakawa, S., Ike, F., Ogawa, T., Tada, T., Kanaya, S., Noce, T., Abe, K.
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Journal Title
Genes Cells 13
Pages: 863-877
Peer Reviewed
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[Journal Article] Targeted chromosome elimination from ES-somatic hybrid cell nuclei.2007
Author(s)
Matsumura, H., Tada, M., Otsuji, T., Yasuchika, K., Nakatsuji, N., Tada, T.
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Journal Title
Nature Methods 4
Pages: 23-25
Peer Reviewed
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