Study on in vivo roles of basophils

2008 Fiscal Year Final Research Report

• Project/Area Number: 19390110
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KARASUYAMA Hajime Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 教授 (60195013)
• Project Period (FY): 2007 – 2008
• Keywords: 炎症 / 好塩基球 / アレルギー / アナフィラキシー

Research Abstract

好塩基球は末梢血白血球のわずか0.5%を占めるに過ぎない最少血球細胞であり、またマスト細胞と類似性があるために、その存在意義が疑問視されていた。私たちは本研究において、好塩基球がIgE依存性慢性アレルギー炎症ならびにIgG依存性全身性アナフィラキシーの責任細胞であることを世界に先駆けて明らかにした。本研究により、生体内において好塩基球とマスト細胞が明らかに異なる役割を果たしていることが証明された。

Research Products

• [Journal Article] Newly-discovered roles for basophils : a neglected minority gains new respect (2009)
  • Author(s): Karasuyama, H., Mukai, K., Tsujimura, Y.and Obata, K.
  • Journal Title: Nat.Rev.Immunol 9 Pages: 9-13
  • Peer Reviewed
• [Journal Article] Fc receptor γ-chain, a constitutive component of the interleukin 3 receptor is required for interleukin 3-induced interleukin 4 (2009)
  • Author(s): Hida, S., Yamasaki, S., Sakamoto, Y., Takamoto, M., Obata, K., Takai, T., Karasuyama, H., Sugane, K., Saito, T.and Taki, S.
  • Journal Title: Nat.Immunol 10 Pages: 214-222
  • Peer Reviewed
• [Journal Article] The skewed heavy-chain repertoire in peritoneal B-1 cells is predetermined by the selection via pre-B cell receptor during B cell ontogeny in the fetal liver (2009)
  • Author(s): Yoshikawa, S., Kawano, Y., Minegishi, Y.and Karasuyama, H.
  • Journal Title: Int.Immunol 21 Pages: 43-52
  • Peer Reviewed
• [Journal Article] New insights into the roles for basophils in acute and chronic allergy (2009)
  • Author(s): Mukai, K., Obata, K., Tsujimura, Y.and Karasuyama, H.
  • Journal Title: Allergol.Int 58 Pages: 11-19
  • Peer Reviewed
• [Journal Article] Defects in Jak-STAT-mediated cytokine signals causehyper-IgE syndrome : lessons from a primary human immunodeficiency (2009)
  • Author(s): Minegishi, Y.and Karasuyama, H.
  • Journal Title: Int.Immunol 21 Pages: 105-112
  • Peer Reviewed
• [Journal Article] Peanuts can contribute to anaphylactic shock by activating complement (2009)
  • Author(s): Khodoun, M., Strait, R., Orekov, T., Hogan,S., Karasuyama, H., Herbert, D. R., Kohl, J.and Finkelman, F.D.
  • Journal Title: J.Allergy Clin.Immunol 58 Pages: 11-19
  • Peer Reviewed
• [Journal Article] LOK is a major ERM kinasein resting lymphocytes and regulates cytoskeletal rearrangement through ERM phosphorylation (2009)
  • Author(s): Belkina N.V., Lie, Y., Hao J-J., Karasuyama,H.and Shaw, S.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 106 Pages: 4707-4712
  • Peer Reviewed
• [Journal Article] 免疫アレルギー研究の表舞台に躍り出た好塩基球 (2009)
  • Author(s): 烏山一
  • Journal Title: アレルギー・免疫 16(3) Pages: 9-10
• [Journal Article] CD200R3を介した好塩基球とマスト細胞の活性化 (2009)
  • Author(s): 小島利之、小畑一茂、烏山一
  • Journal Title: アレルギー・免疫 16(3) Pages: 44-51
• [Journal Article] 好塩基球によるIgG型アナフィラキシーの誘導 (2009)
  • Author(s): 辻村祐佑、小畑一茂、烏山一
  • Journal Title: アレルギー・免疫 16(3) Pages: 62-71
• [Journal Article] 好塩基球が主役を演じる慢性アレルギー炎症 (2009)
  • Author(s): 向井香織、烏山一
  • Journal Title: アレルギー・免疫 16(3) Pages: 72-77
• [Journal Article] Basophils play a pivotal role inimmunoglobulin G-but not immunoglobulinE-mediated systemic anaphylaxis (2008)
  • Author(s): Tsujimura, Y., Obata, K., Mukai, K., Shindou,H., Yoshida, M., Nishikado, H., Kawano, Y.,Minegishi, Y., Shimizu, T.and Karasuyama, H.
  • Journal Title: Immunity 28 Pages: 581-589
  • Peer Reviewed
• [Journal Article] Erk kinases link pre-B cellreceptor signaling to transcriptional events required for early B cell expansion (2008)
  • Author(s): Yasuda, T., Sanjo, H., Pages, G., Kawano, Y.,Karasuyama, H., Pouyssegur, J., Ogata, M.and Kurosaki, T.
  • Journal Title: Immunity 28 Pages: 499-508
  • Peer Reviewed
• [Journal Article] Genetic origins of hyper-IgE syndrome (2008)
  • Author(s): Minegishi, Y.and Karasuyama, H.
  • Journal Title: Curr.Allergy Asthma Rep. 8(5) Pages: 386-91
  • Peer Reviewed
• [Journal Article] Overproduction of IgE induces macrophage-derived chemokine(CCL22)secretion from basophils (2008)
  • Author(s): Watanabe, M., Satoh, T., Yamamoto, Y.,Kanai, Y., Karasuyama, H.and Yokozeki, H.
  • Journal Title: J.Immunol 181 Pages: 5653-5659
  • Peer Reviewed
• [Journal Article] A novel hairless mouse model on anatopic dermatitis-prone genetic backgroundgenerated by receptor-mediated transgenesis (2008)
  • Author(s): Takada, T., Shitara, H., Matsuoka, K.,Kojima, E., Ishii, R., Kikkawa, Y.,Karasuyama, H., Kohno, K.and Yonekawa, H.
  • Journal Title: Transgenic Res. 17(6) Pages: 1155-1162
  • Peer Reviewed
• [Journal Article] 皮膚アレルギーと好塩基球 (2008)
  • Author(s): 横関博雄、烏山一、佐藤貴浩
  • Journal Title: 皮膚アレルギーフロンティア 6(2) Pages: 118-125
• [Journal Article] 好塩基球の21世紀ルネサンス~生体内における好塩基球のユニークな役割が次々と明らかに~ (2008)
  • Author(s): 辻村祐佑、小畑一茂、向井香織、烏山一
  • Journal Title: 臨床血液 49(7) Pages: 489-497
• [Journal Article] アナフィラキシー・ショックを引き起こす新たな仕組み-好塩基球の知られざる役割が明らかに (2008)
  • Author(s): 烏山一
  • Journal Title: Dental Diamond 33 Pages: 66-70
• [Journal Article] アナフィラキシー・ショックを引き起こす新たなメカニズム-好塩基球・IgG・血小板活性化因子を介した即時型アレルギー (2008)
  • Author(s): 辻村祐佑、烏山一
  • Journal Title: 細胞工学 27(10) Pages: 1030-1031
• [Journal Article] アナフィラキシーショック発症の新たなメカニズム (2008)
  • Author(s): 烏山一
  • Journal Title: 日本医事新報 4402 Pages: 45-48
• [Journal Article] 危険なアレルギー反応の新たな仕組みが明らかに (2008)
  • Author(s): 烏山一
  • Journal Title: 科学(岩波書店) 78(11) Pages: 1246-1251
• [Journal Article] 慢性アレルギー炎症ならびにアナフィラキシーにおける好塩基球のあらたな役割 (2008)
  • Author(s): 烏山一
  • Journal Title: 医学のあゆみ 227(5) Pages: 361-366
• [Journal Article] 好塩基球とマスト細胞に発現するCD200R3 (2008)
  • Author(s): 小畑一茂、小島利之、辻村祐佑、向井香織、烏山一
  • Journal Title: 臨床免疫・アレルギー科 50(5) Pages: 595-602
• [Journal Article] Basophils are essentialinitiators of a novel type of chronic allergic inflammation (2007)
  • Author(s): Obata, K., Mukai, K., Tsujimura, Y., Ishiwata,K., Kawano, Y., Minegishi, Y., Watanabe, N.and Karasuyama, H
  • Journal Title: Blood 110 Pages: 913-920
  • Peer Reviewed
• [Journal Article] Dominant-negative mutations in the DNA-binding domain of STAT3 cause hyper-IgE syndrome (2007)
  • Author(s): Minegishi, Y., Saito, M., Tsuchiya, S., Tsuge,I., Takada, H., Hara T., Kawamura, N., Ariga,T., Pasic, S., Stojkovic, O., Metin, A., and Karasuyama, H.
  • Journal Title: Nature 448 Pages: 1058-1062
  • Peer Reviewed
• [Journal Article] Mast cells and basophils are selectively activated in vitro and in vivo through CD200R3 in an IgE-independent manner (2007)
  • Author(s): Kojima, T., Obata, K., Mukai, K., Sato, S.,Takai, T., Minegishi, Y.,and Karasuyama, H.
  • Journal Title: J.Immunol. 179 Pages: 7093-7100
  • Peer Reviewed
• [Journal Article] Hyperimmunoglobulin E syndrome and tyrosine kinasae 2 deficiency (2007)
  • Author(s): Minegishi, Y. and Karasuyama, H.
  • Journal Title: Curr.Opin.Allergy Clin Immunol 7 Pages: 506-509
  • Peer Reviewed
• [Journal Article] 好塩基球によるIgE依存性慢性アレルギー炎症 (2007)
  • Author(s): 向井香織、烏山一
  • Journal Title: アレルギー・免疫(医薬ジャーナル) 14(2) Pages: 40-45
• [Journal Article] 慢性アレルギー炎症における好塩基球の役割 (2007)
  • Author(s): 向井香織、烏山一
  • Journal Title: 皮膚アレルギーフロンティア(メディカルレビュー社) 5(1) Pages: 25-29
• [Journal Article] 好塩基球と慢性アレルギー炎症 (2007)
  • Author(s): 烏山一
  • Journal Title: 医学のあゆみ 220(12) Pages: 949-952
• [Journal Article] 好塩基球が主役を演じる慢性アレルギー炎症 (2007)
  • Author(s): 烏山一
  • Journal Title: メディカル・サイエンス・ダイジェスト(ニューサイエンス社) 33(7) Pages: 26-29
• [Journal Article] 慢性アレルギー炎症における好塩基球の新たな役割 (2007)
  • Author(s): 烏山一
  • Journal Title: 生化学 79(8) Pages: 761-767
• [Journal Article] アレルギーと好塩基球 (2007)
  • Author(s): 烏山一
  • Journal Title: Medical Tribune Vol.40, No.45:43
• [Presentation] Basophils regulate macrophage functions through Th2 cytokines in allergic inflammation (2009)
  • Author(s): Karasuyama, H.
  • Organizer: Allergy and Asthma(Keystone Symposia)
  • Place of Presentation: Keystone, USA
  • Year and Date: 20090120-25
• [Presentation] Basophils regulate macrophage functions through Th2 cytokines (2008)
  • Author(s): Karasuyama H
  • Organizer: The 38^<th> JSI annual meeting
  • Place of Presentation: Kyoto
  • Year and Date: 20081201-03
• [Presentation] Basophils induce alternative activation of macrophages through Th2 cytokine release (2008)
  • Author(s): Mukai K, Minegishi Y, and Karasuyama H
  • Organizer: The 38^<th> JSI annual meeting
  • Place of Presentation: Kyoto
  • Year and Date: 20081201-03
• [Presentation] Novel tools for immunohistochemical identification of mouse basophils in immune and allergic responses : mAbs specific to basophil granular enzymesmMCP-8 and mMCP-11 (2008)
  • Author(s): Ugajin T, Kojima T, Obata K, Mukai K,Kawano Y, Minegishi Y, Yokozeki H, and Karasuyama H
  • Organizer: The 38^<th> JSI annual meeting
  • Place of Presentation: Kyoto
  • Year and Date: 20081201-03
• [Presentation] Basophils play a protective role against Nippostrongylus brasiliensis (2008)
  • Author(s): Obata K, Ishiwata K, Wada T, Kawano Y, Minegishi Y, Watanabe N, and Karasuyama H
  • Organizer: The 38^<th> JSI annual meeting
  • Place of Presentation: Kyoto
  • Year and Date: 20081201-03
• [Presentation] The role for basophils in the protection against external parasites (2008)
  • Author(s): Wada T, Obata K, Ishiwata K, Minegishi Y,Watanabe N,and Karasuyama H
  • Organizer: The 38^<th> JSI annual meeting
  • Place of Presentation: Kyoto
  • Year and Date: 20081201-03
• [Presentation] 特別講演「好塩基球の21世紀ルネサンス~急性ならびに慢性アレルギーにおける好塩基球の新たな役割」 (2008)
  • Author(s): 烏山一
  • Organizer: 第58回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 20081127-29
• [Presentation] 好塩基球由来Th2サイトカインによるalternative activated macrophageの誘導 (2008)
  • Author(s): 向井香織、烏山一
  • Organizer: 第58回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 20081127-29
• [Presentation] The role for basophils in the protection against external parasites (2008)
  • Author(s): Karasuyama H
  • Organizer: The 8^<th> Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji
  • Year and Date: 20080909-10
• [Presentation] 生体防御ならびにアレルギー疾患における好塩基球のユニークな役割 (2008)
  • Author(s): 烏山一
  • Organizer: 第32回阿蘇シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 20080801-02
• [Presentation] Basophils and mast cells play distinct roles in acute and chronic allergic responses (2008)
  • Author(s): Karasuyama H
  • Organizer: FOCIS(Federation of Clinical Immunology Societies)
  • Place of Presentation: Boston, USA
  • Year and Date: 20080605-09
• [Presentation] Roles of mast cells and basophils in the development of allergic diseases (2008)
  • Author(s): Karasuyama H
  • Organizer: Frontiers in Allergy and Autoimmunity
  • Place of Presentation: Mainz,Germany
  • Year and Date: 20080530-31
• [Presentation] Chronic cutaneous allergic inflammation-responsible cells and causative genes (2008)
  • Author(s): Karasuyama H
  • Organizer: International Investigative Dermatology 2008
  • Place of Presentation: Kyoto
  • Year and Date: 20080514-17
• [Presentation] Basophils and mast cells play distinct roles inacute and chronic allergic reactions (2008)
  • Author(s): Karasuyama H, Tsujimura Y, Obata K, MukaiK, Shindo H, Shimizu T, Minegishi Y
  • Organizer: 27^<th> Symposium of the Collegium International Allergologicum
  • Place of Presentation: Curacao, USA
  • Year and Date: 20080501-06
• [Presentation] 見逃されていた好塩基球の重要性 (2008)
  • Author(s): 烏山一
  • Organizer: 第36回日本臨床免疫学会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-18
• [Presentation] 皮膚慢性アレルギー : 責任細胞と原因遺伝子 (2008)
  • Author(s): 烏山一
  • Organizer: 第29回近畿アトピー性皮膚炎懇話会
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-04
• [Presentation] Basophils play a pivotal rolein IgG-but not IgE-mediated systemicanaphylaxis in contrast to mast cells (2008)
  • Author(s): Karasuyama H
  • Organizer: LIAI Allergy Symposium
  • Place of Presentation: La Jolla, USA
  • Year and Date: 2008-04-04
• [Presentation] Novel mechanism of chronic allergic inflammation ; pivotal role of basophils (2007)
  • Author(s): Karasuyama H
  • Organizer: World Allergy Congress 2007
  • Place of Presentation: Bangkok
  • Year and Date: 20071201-06
• [Presentation] Basophils function as initiator rather than effectors in IgE-mediated chronic allergic inflammation (2007)
  • Author(s): Mukai K, Kawano Y, Minegishi Y, Karasuyama H
  • Organizer: The 37^<th> JSI annual meeting
  • Place of Presentation: Tokyo
  • Year and Date: 20071120-23
• [Presentation] A CD200-likeprotein CD200R3 is selectively expressed onbasophils and a subset of mast cells (2007)
  • Author(s): Kojima T, Obata K, Mukai K, Sato S, Minegishi Y, Karasuyama H
  • Organizer: The 37^<th> JSI annual meeting
  • Place of Presentation: Tokyo
  • Year and Date: 20071120-23
• [Presentation] Identification and characterization of murine basophil granularenzymes mMCP-8 and mMCP-11 (2007)
  • Author(s): Ugajin T, Kojima T, Obata K, Tsujimura Y, Mukai K, Kawano Y, Yokozeki H, MinegishiY, Karasuyama H
  • Organizer: The 37^<th> JSI annual meeting
  • Place of Presentation: Tokyo
  • Year and Date: 20071120-23
• [Presentation] CD200R3 functions as an activating receptor expressed exclusively on basophils and a subset of amast cells (2007)
  • Author(s): Obata K, Kojima T, Tsujimura Y, Kawano Y, Minegishi Y.and Karasuyama H
  • Organizer: The 37^<th> JSI annual meeting
  • Place of Presentation: Tokyo
  • Year and Date: 20071120-23
• [Presentation] Basophils and mast cells playdistinct roles in systemic anaphylaxis (2007)
  • Author(s): Karasuyama H, Tsujimura Y, Obata K, Mukai K, Kojima T, Nishikado H, Kawano Y, Minegishi Y
  • Organizer: The 37^<th> JSI annual meeting
  • Place of Presentation: Tokyo
  • Year and Date: 20071120-22
• [Presentation] Basophils play a piovotal rolein IgG-but not IgE-mediated systemic anaphylaxis (2007)
  • Author(s): Tsujimura Y, Kawano Y, Minegishi Y,and Karasuyama H
  • Organizer: The 37^<th> JSI annual meeting
  • Place of Presentation: Tokyo
  • Year and Date: 20071120-22
• [Presentation] Possible functions of basophils in the protection against external parasite (2007)
  • Author(s): Onuma N, Obata K, Ishiwata K, Watanabe N, Minegishi Y.and Karasuyama H
  • Organizer: The 37^<th> JSI annual meeting
  • Place of Presentation: Tokyo
  • Year and Date: 20071120-22
• [Presentation] 慢性アレルギー炎症におけるイニシエーターとしての好塩基球の役割 (2007)
  • Author(s): 向井香織、峯岸克行、烏山一
  • Organizer: 第57回日本アレルギー学会秋季学術大会
  • Place of Presentation: 横浜
  • Year and Date: 20071101-03
• [Presentation] 好塩基球に選択的に発現する顆粒酵素に対する特異抗体の樹立とその応用 (2007)
  • Author(s): 宇賀神つかさ、小島利之、小畑一茂、辻村祐佑、向井香織、河野洋平、峯岸克行、烏山一
  • Organizer: 第57回日本アレルギー学会秋季学術大会
  • Place of Presentation: 横浜
  • Year and Date: 20071101-03
• [Presentation] 好塩基球が中心的な役割を果たすIgG依存性全身性アナフィラキシー (2007)
  • Author(s): 辻村祐佑、河野洋平、峯岸克行、烏山一
  • Organizer: 第57回日本アレルギー学会秋季学術大会
  • Place of Presentation: 横浜
  • Year and Date: 20071101-03
• [Presentation] CD200R3は好塩基球・マスト細胞特異的な活性型受容体として機能する (2007)
  • Author(s): 小畑一茂、小島利之、辻村祐佑、峯岸克行、烏山一
  • Organizer: 第57回日本アレルギー学会秋季学術大会
  • Place of Presentation: 横浜
  • Year and Date: 20071101-03
• [Presentation] Basophils function as an initiator of chronic allergic inflammation (2007)
  • Author(s): Karasuyama H, Mukai K, Obata K, TsujimuraY, Kawano Y, Ishiwata K, Watanabe N, and Minegishi Y
  • Organizer: The 20^<th> Naito Conference
  • Place of Presentation: Kanagawa
  • Year and Date: 20071009-12
• [Presentation] 即時型ならびに慢性アレルギー反応における好塩基球の新たな役割 (2007)
  • Author(s): 烏山一
  • Organizer: 生体機能と創薬シンポジウム2007
  • Place of Presentation: 金沢
  • Year and Date: 20070913-14
• [Presentation] Basophils functions as initiators rather than effectors in a novel type of chronic allergic inflammation (2007)
  • Author(s): Karasuyama H
  • Organizer: The 13th International Congress of Immunology
  • Place of Presentation: Rio de Janeiro
  • Year and Date: 20070821-25
• [Presentation] 慢性アレルギー性炎症における好塩基球の役割 (2007)
  • Author(s): 烏山一
  • Organizer: 第17回国際喘息学会日本北アジア部会
  • Place of Presentation: 東京
  • Year and Date: 20070706-07
• [Presentation] The traffic of basophils in allergic responses (2007)
  • Author(s): Karasuyama H
  • Organizer: The 28^<th> Congress of the European Academy of Allergology and Clinical Immunology
  • Place of Presentation: Goteborg
  • Year and Date: 20070609-13
• [Presentation] Basophils function as initiators rather than effectors in IgE-mediated chronic allergic inflammation, independently of T cells andmast cells (2007)
  • Author(s): Karasuyama H, Mukai K, Obata K, Ishiwata K, Watanabe N, Kawano Y, Minegishi Y
  • Organizer: The 94^<th> annual meeting of the American Association of Immunologists
  • Place of Presentation: Miami
  • Year and Date: 20070518-22
• [Book] 「アレルギーにおける好塩基球の新たな役割」Annual Review 2009 呼吸器 (2009)
  • Author(s): 烏山一
  • Total Pages: 44-51
  • Publisher: 中外医学社
• [Book] 「アレルギー性炎症」免疫応答と免疫病態の総合的分子理解 (2007)
  • Author(s): 烏山一
  • Total Pages: 189-196
  • Publisher: 南山堂
• [Remarks]
  • URL: http://www.tmd.ac.jp/med/mbch/top_Japanese.htm