Roles of phosphorylation signals in regulation of stem cell systems

2008 Fiscal Year Final Research Report

• Project/Area Number: 19390113
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Osaka University
• Principal Investigator: KIMURA Tohru Osaka University, 医学系・研究科, 准教授 (50280962)
• Co-Investigator(Kenkyū-buntansha): KITAJIMA Kenji 大阪大学, 医学系・研究科, 特任准教授(常勤) (10346132)
• Co-Investigator(Renkei-kenkyūsha): TARUTANI Masahito 兵庫医科大学, 医学部, 講師 (30301261)
• Project Period (FY): 2007 – 2008
• Keywords: 幹細胞システム / リン酸化酵素 / シグナル伝達 / 組織幹細胞 / 胚性幹細胞 / 生殖細胞 / 脱分化 / 初期化

Research Abstract

リン酸化シグナルPI3K (Phosphoinositide 3 kinase) /Aktシグナルが、毛包幹細胞の休止・活性化や軟骨の最終分化を制御すること、Aktによる癌抑制遺伝子p53の機能抑制が生殖細胞の脱分化を促進すること、GSK3 (glycogen synthase kinase 3)シグナルの阻害はES細胞の樹立効率を上昇させることなどを明らかにした。各幹細胞システムにおけるリン酸化シグナルの機能の特異性・共通性を解明し、応用研究への基盤を整えた。

Research Products

• [Journal Article] Effects of Akt signaling on nuclear reprogramming (2008)
  • Author(s): Nakamura T, Inoue K, Ogawa S, Umehara H, Ogonuki N, Miki H, Kimura T, Ogura A, and Nakano T.
  • Journal Title: Genes Cells 13(12) Pages: 1269-1277
  • Peer Reviewed
• [Journal Article] PI3K/Akt signaling as a key regulatory pathway for chondrocyte terminal differentiation (2008)
  • Author(s): Kita K, Kimura T, Nakamura N, Yoshikawa H, and Nakano T.
  • Journal Title: Genes Cells 13(8) Pages: 839-850
  • Peer Reviewed
• [Journal Article] DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes (2008)
  • Author(s): Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Totoki Y, Toyoda A, Ikawa M , Asada N, Kojima K, Yamaguchi Y, Ijiri T, Hata K, Li E, Matsuda Y, Kimura T, Okabe M, Sakaki Y, Sasaki H, and Nakano T.
  • Journal Title: Genes Dev 22(7) Pages: 908-917
  • Peer Reviewed
• [Journal Article] AKT signaling promotes derivation of embryonic germ cells form primordial germ cells (2008)
  • Author(s): Kimura T, Tomooka M, Yamano N, Murayama K, Matoba S, Umehara H, Kanai Y, and Nakano T.
  • Journal Title: Development 135(5) Pages: 869-879
  • Peer Reviewed
• [Journal Article] Efficient derivation of embryonic stem cells by inhibition of glycogen synthase kinase 3 (2007)
  • Author(s): Umehara H, Kimura T, Ohtsuka S, Nakamura T, Kitajima K, Ikawa M, Okabe M, Niwa H, and Nakano T.
  • Journal Title: Stem Cells 25(11) Pages: 2705-2711
  • Peer Reviewed
• [Journal Article] Akt mediates self-renewal division of mouse spermatogonial stem cells (2007)
  • Author(s): Lee J, Shinohara-Kanatsu M, Inoue K, Ogonuki N, Miki H, Toyokuni S, Kimura T, Nakano T, Ogura A, and Shinohara T.
  • Journal Title: Development 134(10) Pages: 1853-1859
  • Peer Reviewed
• [Journal Article] Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors (2007)
  • Author(s): Murayama K, Kimura T, Tarutani M, Tomooka M, Hayashi R, Okabe M, Nishida K, Itami S, Katayama I, and Nakano T.
  • Journal Title: Oncogene 26(33) Pages: 4882-4888
  • Peer Reviewed
• [Journal Article] PGC7/Stella protects against DNA demethylation in early embryogenesis (2007)
  • Author(s): Nakamura T, Arai Y, Umehara H, Masuhara M, Kimura T, Taniguchi H, Sekimoto T, Ikawa M, Yoneda Y, Okabe M, Tanaka S, Shiota K, and Nakano T.
  • Journal Title: Nature Cell Biol 9(1) Pages: 64-71
  • Peer Reviewed
• [Presentation] PI3K/Aktシグナルによる多能性幹細胞システムの制御 (2008)
  • Author(s): 木村透、中村肇伸、山野範子、山本敏之、鈴木聡、仲野徹
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会合同学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 20081209-12
• [Presentation] Regulation of Pluripotency in Mouse Primordial Germ Cells (2008)
  • Author(s): Kimura T, Yamano N, Yamamoto T, and Nakano T.
  • Organizer: Cold Spring Harbor Symposium Germ Cell Meeting
  • Place of Presentation: Cold Spring Harbor Laboratory
  • Year and Date: 20081001-05
• [Presentation] 分化多能性の獲得と維持におけるPI3K/Aktシグナルの機能 (2008)
  • Author(s): 木村透、中村肇伸、山野範子、鈴木聡、仲野徹
  • Organizer: 第6回幹細胞シンポジウム
  • Place of Presentation: 学術総合センター
  • Year and Date: 20080516-17
• [Presentation] PI3K-Aktシグナルによる幹細胞システム制御 (2007)
  • Author(s): 木村透、鈴木聡、仲野徹
  • Organizer: 第40回日本発生生物学会、第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20070528-30
• [Presentation] Aktシグナルによる表皮幹細胞の活性化と前駆細胞の増幅 (2007)
  • Author(s): 木村透、村山一茂、樽谷勝仁、林竜平、岡部勝、板見智、片山一朗、仲野徹
  • Organizer: 第5回幹細胞シンポジウム
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 20070517-19
• [Book] Regulation of stem cell systems by PI3K/Akt signaling (2009)
  • Author(s): Kimura T, and Nakano T.
  • Total Pages: 309-317
  • Publisher: Humana Press