2009 Fiscal Year Final Research Report
Diagnostic value of peptidome analyses for protease activated pathological conditions beyond cancer diagnosis
| Project/Area Number |
19390156
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kagoshima University |
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Principal Investigator |
HASHIGUCHI Teruto Kagoshima University, 大学院・医歯学総合研究科, 准教授 (70250917)
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| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Ikuro 鹿児島大学, 大学院・医歯学総合研究科, 教授 (20082282)
KAWAHARA Koichi 鹿児島大学, 大学院・医歯学総合研究科, 助教 (10381170)
INUI Akio 鹿児島大学, 大学院・医歯学総合研究科, 教授 (80168418)
NATSUGOE Shoji 鹿児島大学, 大学院・医歯学総合研究科, 教授 (70237577)
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| Research Collaborator |
TANAKA Kenji , (株)プロトセラ・膜タンパク質&リガンド解析センター
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| Project Period (FY) |
2007 – 2009
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| Keywords | プロテオーム / ペプチドーム / ペプチド / バイオマーカー / 質量分析 / 癌 / DIC / 検査 |
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| Research Abstract |
Human serum contains thousands of proteolytically derived low-molecular-weight peptide fragments (serum peptidome). The concept of utilizing the serum peptidome for cancer diagnosis has been developed. A pathological serum peptidome appears when the homeostatic balance between proteases and protease inhibitors is disrupted. We hypothesize if analyses of the serum peptidome are of diagnostic value as information on which molecules are disrupted, and the pathological course it will take in unknown pathological conditions and disseminated intravascular coagulation (DIC). We analyzed the serum peptidome in 3 stages (early stage, pre-DIC and DIC stages) in one patient with POEMS (polyneuropathy, organomegaly, endocrinopathy, M protein and skin changes) syndrome, an intractable disease with unknown pathology, using a 1-dimensional gel electrophoresis/ matrix-assisted laser desorption/ionization-mass spectrometry (1-DE/MS)-based rapid quantitative approach. A very large number of peptide fragments appeared in the DIC stage, compared to pre-DIC. In addition, we identified fragments of transthyretin (ALGISPFHEHAEVVFTANDSGPR, m/z 2,451.18) and α1-antitrypsin (EDPQGDAAQKTDTSHHDQDHPTFN, m/z 2,691.02) that significantly increased in the DIC stage, compared to those in the pre-DIC stage. Rapid analyses of the serum peptidome may lead to a diagnostic method that can predict on-going protease activated pathological conditions and help to decide on multilateral strategies including nutritional support and drug therapy.
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[Journal Article] B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes.2010
Author(s)
Hashiguchi T*, Shrestha B*, Ito T*, Miura N* (*Equal contribution), Takenouchi K, Oyama Y, Kawahara K, Tancharoen S, Ki-i Y, Arimura N, Yoshinaga N, Noma S, Shrestha C, Nitanda T, Kitajima S, Arimura K, Sato M, Sakamoto T, Maruyama I
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Journal Title
J Immunol 184
Pages: 4819-4826
Peer Reviewed
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[Journal Article] Intraocular expression and release of high-mobility group box 1 protein in retinal detachment.2009
Author(s)
Arimura N, Ki-i Y, Hashiguchi T, Kawahara K, Biswas KK, Nakamura M, Sonoda Y, Yamakiri K, Okubo A, Sakamoto T, Maruyama I
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Journal Title
Lab Invest 89
Pages: 278-289
Peer Reviewed
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[Journal Article] Association between reduced ADAMTS13 and diabetic nephropathy
Author(s)
Taniguchi S, Hashiguchi T, Ono T, Takenouchi K, Nakayama K, Kawano T, Kato K, Matsushita R, Nagatomo M, Nakamura S, Nakashima T, Maruyama I
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Journal Title
Peer Reviewed
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[Journal Article] High-mobility group box-1 protein promotes granulomatous nephritis in adenine-induced nephropathy.
Author(s)
Oyama Y, Hashiguchi T, Taniguchi N, Tancharoen S, Uchimura T, Biswas KK, Kawahara K, Nitanda T, Umekita Y, Lotz M, Maruyama I
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Journal Title
Peer Reviewed
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