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2008 Fiscal Year Final Research Report

Development of serum tumor markers by using antibodies generated by Top-Down screening strategy

Research Project

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Project/Area Number 19390158
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKitasato University

Principal Investigator

MAEDA Tadakazu  Kitasato University, 理学部, 教授 (90265728)

Co-Investigator(Kenkyū-buntansha) SATO Yuichi  北里大学, 医療衛生学部, 准教授 (30178793)
BABA Shiro  北里大学, 医学部, 教授 (00051889)
MATSUMOTO Kazumasa  北里大学, 医学部, 講師 (70306603)
KODERA Yoshio  北里大学, 理学部, 准教授 (60265733)
Co-Investigator(Renkei-kenkyūsha) NOZAKI Naohito  神奈川歯科大学, 歯学部, 講師 (70222198)
Project Period (FY) 2007 – 2008
Keywords臨床検査医学
Research Abstract

ヒト腫瘍部位の組織もしくは樹立癌細胞株から取得したタンパク質混合物をそのままマウスに免疫し、取り出した脾細胞に対して常法に従い細胞融合し、限界希釈法を経てモノクローナル抗体を獲得する(ランダム免疫法)。我々の最終目的は低侵襲性で患者への負担の少ない体液(血液、尿)を対象とした癌診断法の確立である。本研究ではランダム免疫法で獲得した抗体について、免疫ブロットならびに組織染色によって、有用抗体を評価した

  • Research Products

    (41 results)

All 2009 2008 2007 Other

All Journal Article (21 results) (of which Peer Reviewed: 21 results) Presentation (15 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 2 results)

  • [Journal Article] A simple and highly reproducible method for discovering potential disease markers in low abundance serum proteins2009

    • Author(s)
      Kawashima, Y., Fukuno T., Satoh M., Takahashi H., Matsui T., Maeda T., and Kodera Y.
    • Journal Title

      J. Electrophoresis 57

      Pages: 13-18

    • Peer Reviewed
  • [Journal Article] Hoffman RM. Human and mouse hair follicles contain both multipotent and monopotent stem cells2009

    • Author(s)
      Amoh Y, Sato Y, Katsuoka K(他3名)
    • Journal Title

      Cell Cycle 8

      Pages: 1-2

    • Peer Reviewed
  • [Journal Article] Expression of the hair stem cell-specific Marker nestin in epithelial and follicular tumors2008

    • Author(s)
      Kanoh M,Amoh Y, Sato Y, Katsuoka K.
    • Journal Title

      Eur J Dermatol 18(5)

      Pages: 518-523

    • Peer Reviewed
  • [Journal Article] Detection of tumor-specific autoantibodies in sera of patients with lung cancer2008

    • Author(s)
      Nagashio R, Sato Y, Jiang Shi-Xu Ryuge S, Kodera Y, Maeda T, Nakajima T.
    • Journal Title

      Lung Cancer 62

      Pages: 364-373

    • Peer Reviewed
  • [Journal Article] MAGE-A protein and MAGE-A10 gene expression in liver metastasis in patients with stomach cancer2008

    • Author(s)
      Suzuki S, Sato Y, Tsukui T(他5名)
    • Journal Title

      Br J Cancer 99

      Pages: 350-356

    • Peer Reviewed
  • [Journal Article] A lymphatic vessel network in normal urothelium of the urinary bladder2008

    • Author(s)
      Matsumoto K, Soh S, Satoh T, Iwamura M, Ishikawa Y, Ishii T, Baba S.
    • Journal Title

      Urology 12

      Pages: 706-710

    • Peer Reviewed
  • [Journal Article] Recent advances in molecular markers for bladder cancer2008

    • Author(s)
      Matsumoto K, Matsushita K, Baba S (他5名)
    • Journal Title

      Res Adv in Urol 1

      Pages: 1-17

    • Peer Reviewed
  • [Journal Article] Loss expression of uroplakin III is associated with clinicopathological features of aggressive bladder cancer2008

    • Author(s)
      Matsumoto K, Baba S (他5名)
    • Journal Title

      Urology 72

      Pages: 444-449

    • Peer Reviewed
  • [Journal Article] Protein Carbonyl Detection by Two-Dimensional Fluorescence Difference Gel Electrophoresis2008

    • Author(s)
      Sawada A., Kodera Y. and Maeda T.(他4名)
    • Journal Title

      J. Electrophoresis 52

      Pages: 1-17

    • Peer Reviewed
  • [Journal Article] Searching for new biomarkers of bladder cancer based on proteomic analysis2008

    • Author(s)
      Okusa H., Kodera Y., Oh-ishi M., Minamida M., Tsuchida M., Kavoussi N., Matsumoto K., Sato T., Iwamura M., Maeda T. and Baba S.
    • Journal Title

      J. Electrophoresis 52

      Pages: 19-24

    • Peer Reviewed
  • [Journal Article] Identification of novel immunohistochemical tumor markers for primary hepatocellular carcinoma ; clathrin heavy chain and formiminotransferase cyclodeaminase2008

    • Author(s)
      Seimiya M., Kodera Y., Maeda T., Nomura F.(他11名)
    • Journal Title

      Hepatology 48

      Pages: 519-530

    • Peer Reviewed
  • [Journal Article] Detection of tumor-specific autoantibodies in sera of patients with lung cancer2008

    • Author(s)
      Nagashio R, Sato Y, Jiang SX, Ryuge S, Kodera Y, Maeda T, Nakajima T.
    • Journal Title

      Lung Cancer 62

      Pages: 364-73

    • Peer Reviewed
  • [Journal Article] Disease proteomics of endocrine disorders revealed by two-dimensional gel electrophoresis and mass spectrometry2008

    • Author(s)
      Oh-Ishi M, Kodera Y, Furudate S, Maeda T.
    • Journal Title

      Proteomics Clin Appl 2

      Pages: 327-337

    • Peer Reviewed
  • [Journal Article] Expression of S100A2 and S100A4 predicts disease progression and patient survival in bladder cancer2007

    • Author(s)
      Matsumoto K, Baba S.(他6名)
    • Journal Title

      Urology 70

      Pages: 602-607

    • Peer Reviewed
  • [Journal Article] Gemcitabine and paclitaxel chemotherapy as a second-line treatement for advanced transitionalcell carcinoma2007

    • Author(s)
      Matsumoto K, Irie A, Satoh T, Okazaki M, Iwamura M, Baba S.
    • Journal Title

      Int J Urol 14

      Pages: 1000-1004

    • Peer Reviewed
  • [Journal Article] Structural role of the secondary active domain of HIV-2 NCp8 in multi-functionality. Biochem2007

    • Author(s)
      Matsui T., Kodera Y., Maeda T. (他6名)
    • Journal Title

      Biophys. Res. Commun 678

      Pages: 373-678

    • Peer Reviewed
  • [Journal Article] RNA Recognition Mechanism of the Minimal Active Domain of the Human Immunodeficiency Virus Type-2 Nucleocapsid Protein2007

    • Author(s)
      Matsui T., Kodera Y., and Maeda T.(他6名)
    • Journal Title

      J Biochem 141

      Pages: 269-277

    • Peer Reviewed
  • [Journal Article] Detection of biomarkers for alcoholism by two-dimensional differential gel electrophoresis2007

    • Author(s)
      Wu D, Kodera Y, Maeda T, Nomura F.(他7名)
    • Journal Title

      Alcohol Clin Exp Res. 31

      Pages: s67-71

    • Peer Reviewed
  • [Journal Article] A Search for Novel Markers of Alcohol Abuse Using Magnetic beads and MALDI-TOF /TOF Mass Spectrometry

    • Author(s)
      Sogawa K., Kodera Y., Nomura F.(他3名)
    • Journal Title

      Proteomics-Clinical applications (in press)

    • Peer Reviewed
  • [Journal Article] YKL-40 identified by proteomic analysis as a biomarker of sepsis

    • Author(s)
      Hattori N, Kodera Y, Sogawa K, Satoh M, Hirasawa H.(他8名)
    • Journal Title

      Shock (in press)

    • Peer Reviewed
  • [Journal Article] The RNA recognition mechanism of human immunodeficiency virus (HIV) type 2 NCp8 is different from that of HIV-1 NCp7

    • Author(s)
      Matsui T., Maeda M., and Kodera Y., (他9名)
    • Journal Title

      Biochemistry (in press)

    • Peer Reviewed
  • [Presentation] 質量分析計を用いた診断を目指して2009

    • Author(s)
      小寺義男
    • Organizer
      第11回次世代医療システム産業化フォーラム2008(招待)
    • Place of Presentation
      大阪商工会議所
    • Year and Date
      2009-02-19
  • [Presentation] Development of the basic strategy for the comparative analysis of serum proteomes and its application in thediscovery of hepatic injury biomarkers2008

    • Author(s)
      Kodera, Y., Maeda, T.(他9名)
    • Organizer
      17th Meeting of Methods in Protein Structure Analysis (MPSA2008)
    • Place of Presentation
      Sapporo
    • Year and Date
      20080726-29
  • [Presentation] 独自の基盤技術の確立と臨床応用可能なプロダクトの創出を目指して2008

    • Author(s)
      小寺義男
    • Organizer
      第35回BMSコンファランス(招待)
    • Place of Presentation
      裏磐梯ロイヤルホテル
    • Year and Date
      20080706-09
  • [Presentation] Development of novel serum peptidomic strategy and its application in the discovery of colon cancer tumor marker peptides2008

    • Author(s)
      Kodera, Y., Maeda, T.(他6名)
    • Organizer
      PepTalk2008
    • Place of Presentation
      San-Diego
    • Year and Date
      20080106-09
  • [Presentation] Use of gel-based low molecular weight proteomic approach to discover potential biomarkers in serum2008

    • Author(s)
      Kawashima, Y., Kodera, Y., Maeda, T.(他8 名)
    • Organizer
      PepTalk2008
    • Place of Presentation
      San-Diego
    • Year and Date
      20080106-09
  • [Presentation] Development of the basic strategy for the comparative analysis of serum proteomes to discover potential biomarkers2008

    • Author(s)
      Fukuno, T., Kodera, Y., Maeda, T., (他9名)
    • Organizer
      PepTalk2008
    • Place of Presentation
      San-Diego
    • Year and Date
      20080106-09
  • [Presentation] 血清中のペプチドを対象とした診断法の確立を目指して2008

    • Author(s)
      小寺義男(シンポジウム)
    • Organizer
      日本分析化学会 第217 回液体クロマトグラフィー研究懇談会
    • Place of Presentation
      東京理科大学
    • Year and Date
      2008-11-21
  • [Presentation] 特別講演 : 北里大学での疾患プロテオミクスの歩み、これまでとこれから Clinical Proteomics in Chiba 20082008

    • Author(s)
      前田忠計(招待)
    • Organizer
      第5回千葉疾患プロテオミクス研究会
    • Place of Presentation
      千葉
    • Year and Date
      2008-11-16
  • [Presentation] 独自のペプチド分析法による大腸癌マーカーペプチドの探索と評価2008

    • Author(s)
      小寺義男, 川島祐介, 福冨俊之, 高橋広樹, 相野谷学, 丸橋正弘, 松井崇, 平賀啓介, 曽川一幸, 朝長毅, 野村文夫, 前田忠計
    • Organizer
      日本ヒトプロテオーム機構(JHUPO)第6回大会(シンポジウム)
    • Place of Presentation
      大阪府茨木市
    • Year and Date
      2008-07-30
  • [Presentation] 量子ドット技術を用いた複数のタンパク質翻訳後修飾の同時検出2008

    • Author(s)
      大石正道, 小寺義男, 前田忠計
    • Organizer
      日本ヒトプロテオーム機構(JHUPO)第6回大会(シンポジウム)
    • Place of Presentation
      大阪府茨木市
    • Year and Date
      2008-07-29
  • [Presentation] 体液を対象とした診断システムの開発2008

    • Author(s)
      小寺義男
    • Organizer
      第58回日本電気泳動学会シンポジウム(シンポジウム)
    • Place of Presentation
      東京都港区
    • Year and Date
      2008-06-13
  • [Presentation] 有用プロダクト創出を目指した疾患プロテオーム解析 第58 回日本電気泳動学会シンポジウム2008

    • Author(s)
      前田忠計
    • Organizer
      基礎研究から様々な応用研究へ飛躍する最先端プロテオミクス(シンポジウム)
    • Place of Presentation
      東京都港区
    • Year and Date
      2008-06-13
  • [Presentation] 尿を対象としたプロテオミクスの最前線2007

    • Author(s)
      小寺義男(招待)
    • Organizer
      日本臨床検査自動化学会第39回大会
    • Place of Presentation
      横浜
    • Year and Date
      20070926-28
  • [Presentation] High molecular mass proteomics of human renal cell carcinoma by 2-DE using agarose gels for IEF (Agarose 2-DE) and HPLC/MS/MS HUPO 6th Annual World Congress2007

    • Author(s)
      Oh-Ishi M, Kodera Y, Baba S, Maeda T (他4名)
    • Organizer
      第6回国際ヒトプロテオーム機構年会
    • Place of Presentation
      Seoul, Korea : 韓国, ソウル
    • Year and Date
      2007-10-09
  • [Presentation] Urinary proteomics of high-molecular-mass proteins by 2-DE with agarose gels in the first dimension (Agarose 2-DE) and LC-MS/MS Human Kidney & Urine Proteome Project2007

    • Author(s)
      Okusa H, Kodera Y, Kondo R, Matsumoto K, Iwamura M, Oh-Ishi M, Baba S, Maeda T
    • Organizer
      HUPO 6^<th> Annual World Congress
    • Place of Presentation
      Seoul, Korea : 韓国, ソウル
    • Year and Date
      2007-10-06
  • [Patent(Industrial Property Rights)] 大腸癌マーカーペプチド、及び大腸癌の診断2008

    • Inventor(s)
      小寺義男、川島祐介、前田忠計、朝長毅、野村文夫
    • Industrial Property Rights Holder
      学校法人北里研究所
    • Industrial Property Number
      PCT/JP2008/002217
    • Filing Date
      2008-08-15
    • Overseas
  • [Patent(Industrial Property Rights)] 体液試料における、低分子量タンパク質及びペプチドの濃縮方法2008

    • Inventor(s)
      小寺義男、川島裕介、前田忠計
    • Industrial Property Rights Holder
      学校法人北里研究所
    • Industrial Property Number
      PCT/JP2008/002168
    • Filing Date
      2008-08-08
    • Overseas
  • [Patent(Industrial Property Rights)] 膀胱癌の診断2008

    • Inventor(s)
      松本和将、大草洋、馬場志郎、小寺義男、前田忠計
    • Industrial Property Rights Holder
      学校法人北里研究所
    • Industrial Property Number
      特願2008-066122
    • Filing Date
      2008-03-14
  • [Patent(Industrial Property Rights)] 大腸癌マーカーペプチド、及び大腸癌の診断2007

    • Inventor(s)
      小寺義男、川島祐介、前田忠計、朝長毅、野村文夫
    • Industrial Property Rights Holder
      学校法人北里学園、国立大学法人千葉大学
    • Industrial Property Number
      特願2007-213211
    • Filing Date
      2007-08-18
  • [Patent(Industrial Property Rights)] 体液試料における、低分子量タンパク質及びペプチドの濃縮方法2007

    • Inventor(s)
      小寺義男、川島裕介、前田忠計
    • Industrial Property Rights Holder
      学校法人北里学園
    • Industrial Property Number
      特願2007-206602
    • Filing Date
      2007-08-08

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Published: 2010-06-10   Modified: 2016-04-21  

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