Basic analysis of new collectins in renal tract.

2009 Fiscal Year Final Research Report

• Project/Area Number: 19390227
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Asahikawa Medical College
• Principal Investigator: WAKAMIYA Nobutaka Asahikawa Medical College, 医学部・医学科, 教授 (20210867)
• Co-Investigator(Kenkyū-buntansha): OHTANI Katsuki 旭川医科大学, 医学部, 助教 (90396367) ; SUZUKI Ysuhiko 北海道大学, 人獣共通感染症リサーチセンター, 教授 (90206540) ; YOHIDA Itsuro 旭川医科大学, 医学部, 准教授 (20041816) ; MOTOMURA Wataru 旭川医科大学, 医学部, 助教 (70374791) ; JANG Seong-jae 旭川医科大学, 医学部, 特任助教 (50466497)
• Project Period (FY): 2006 – 2009
• Keywords: 腎疾患 / コラーゲン / レクチン

Research Abstract

初年度において、各種リコンビナントコレクチン作成とその抗体作成を行った。遺伝子改変動物の作成については、ノックアウトマウスはCL-P1、CL-K1について両者とも、キメラマウスが樹立でき、さらにヘテロマウス作成が終了し、精子保存にてノックアウト表現型解析に進んでいる。平成22年度末の時点で、CL-P1は、仔マウスとして誕生せず、胎生致死に関与する遺伝子であると考えられた。一方、CL-K1ノックアウトマウスは無事誕生し、一見正常に見えた。しかしながら、ノックアウトマウス個体が野生型の通常マウスに比較して小型であることや、誕生個体が少ないことより、個体発生や生育に関与する遺伝子である可能性がでている。腎組織おける検討では、局在は異なるが両コレクチンの存在することが、本研究により再確認されたが、上記マウスを用いた病態における意義については十分な解析結果が得られていない、今後の検討が待たれる。

Research Products

• [Journal Article] Mannose binding lectin and lung collectins interact with Toll-like receptor 4 and MD-2 by different mechanisms (2009)
  • Author(s): Shimizu, T., Nishitani, C., Mitsuzawa, H., Ariki,S., Takahashi, M., Ohtani, K., Wakamiya, N., Kuroki, Y.
  • Journal Title: B.B.A. 1790 Pages: 1705-1710
• [Journal Article] Scavenger receptor collectin placenta 1(CL-P1)predominantly mediates zymosan phagocytosis by human vascular endothelial cells (2009)
  • Author(s): Jang S-J, Ohtani K, Fukuoh A, Yoshizaki T, Fukuda M, Motomura W, Mori K, Fukuzawa J, Kitamaoto N, Yoshida I, Suzuki Y, Wakamiya, N.
  • Journal Title: J. Biol. Chem. 284(6) Pages: 3956-65
• [Journal Article] Troglitazone increases expression of E-cadherin and claudin 4 in human pancreatic cancer cells (2009)
  • Author(s): Kumei S, Motomura W, Yoshizaki T, Takakusaki K, Okumura T
  • Journal Title: Biochem Biophys Res Commun. 380(3) Pages: 614-9
• [Journal Article] A Jak2 inhibitor, AG490, reverses lipin-1 suppression by TNF-alpha in 3T3-L1 adipocytes (2009)
  • Author(s): Tsuchiya Y, Takahashi N, Yoshizaki T, Tanno S, Ohhira M, Motomura W, Tanno S, Takakusaki K, Kohgo Y, Okumura T.
  • Journal Title: Biochem Biophys Res Commun. 382(2) Pages: 348-52
• [Journal Article] Loop-Mediated Isothermal Amplification (LAMP) for the direct detection of human pulmonary infections with environmental (nontuberclosis) mycobacteria (2009)
  • Author(s): Adhikari BR, Pandy BD, Ghimire P, Shrestha B, Khadka M, Yoda T, Suzuki Y.
  • Journal Title: Jpn. J. Infect. Dis. 62 Pages: 212-214
• [Journal Article] Immunolocalization of a novel collectin CL-K1 in murine tissues (2008)
  • Author(s): Motomura W, Yoshizaki T, Ohtani K, Okumura T, Fukuda M, Fukuzawa J, Mori K, Jang SJ, Nomura N, Yoshida I, Suzuki Y, KohgoY, Wakamiya, N
  • Journal Title: J Histochem Cytochem 56(3) Pages: 243-52
• [Journal Article] Detection of culturable and nonculturable Legionella species from hot water systems of public buildings in Japan (2008)
  • Author(s): Edagawa A, Kimura A, Doi H, Tanaka H, Tomioka K, Sakabe K, Nakajima C, Suzuki Y.
  • Journal Title: J. Appl. Microbiol. 105 Pages: 2104-2114
• [Journal Article] A novel method to simply detect mutations conferring drug resistance in Mycobacterium leprae based on a DNA microarray, and its applicability in developing countries (2008)
  • Author(s): Matsuoka M, Aye KS, Kyaw K, Tan EV, Balagon MV, Saunderson P, Makino M, Nakajima C, Suzuki Y
  • Journal Title: J. Med. Microbiol. 57 Pages: 1213-1219
• [Journal Article] Development of an in-house loop-mediated isothermal amplification (LAMP) assay for detection of Mycobacterium tuberculosis and evaluation in sputum samples of Nepalese patients (2008)
  • Author(s): Pandey BD, Poudel A, Yoda T, Tamaru A, Oda N, Fukushima Y, Lekhak B, Risal B, Acharya B, Sapkota B, Nakajima C, Taniguchi T, Phetsuksiri B, Suzuki Y.
  • Journal Title: J. Med. Microbiol. 57 Pages: 439-43
• [Journal Article] Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily (2008)
  • Author(s): Higuchi M, Matsuo A, Shingai M, Shida K, Ishii A, Funami K, Suzuki Y, Oshiumi H, Matsumoto M, Seya T.
  • Journal Title: Dev. Comp. Immunol. 32 Pages: 147-55
• [Journal Article] Anti-influenza A virus activities of mannan-binding lectins and bovine conglutinin (2007)
  • Author(s): Kawai T, Kase T, Suzuki Y, Eda S, Sakamoto T, Ohtani K, Wakamiya, N.
  • Journal Title: J Vet Med Sci. 69(2) Pages: 221-4
• [Journal Article] Lipopolysaccharide induces adipose differentiation-related protein expression and lipid accumulation in the liver through inhibition of fatty acid oxidation in mice (2007)
  • Author(s): Ohhira M, Motomura W, Fukuda M, Yoshizaki T, Takahashi N, Tanno S, Wakamiya, N., Kohgo Y, Kumei S, Okumura T.
  • Journal Title: J Gastroenterol. 42 Pages: 969-78
• [Journal Article] Evaluation and Application of Detecting Norovirus using Reverse Transcription Loop-Mediated Isothermal Amplification (2007)
  • Author(s): Yoda T, Suzuki Y, Yamazaki K, Sakon N, Kanki M, Aoyama I, Tsukamoto T.
  • Journal Title: J. Med. Virol. 79 Pages: 326-334
• [Journal Article] Association of the Rv0679c protein with lipids and carbohydrates in Mycobacterium tuberculosis/Mycobacterium bovis BCG (2007)
  • Author(s): Matsuba T, Suzuki Y, Tanaka Y.
  • Journal Title: Arch. Microbiol 187 Pages: 297-311
• [Presentation] Molecular Cloning and Characterization of Novel Ascidian Collectins (2009)
  • Author(s): K Ohtani, K Azumi, S-J Jang, K Mori, W Motomura, I Yoshida, Y Suzuki, N. Wakamiya, N.
  • Place of Presentation: Prague(Czech Republic)
  • Year and Date: 20090600
• [Presentation] Cloning and characterization of novel Ascidian collectins (2008)
  • Author(s): Wakamiya, N., Ohtani K, Azumi K, Jang S-J, Mori K, Fukuzawa J, Yoshida I, Suzuki Y.
  • Organizer: INTERLEC-23
  • Place of Presentation: Stirling(Scotland)
  • Year and Date: 20080700
• [Book] The novel collectins, CL-L1, CL-P1 and CL-K1. COLLAGEN-RELATED LECTINS IN INNATE IMMUNITY(Kilpatrick D. edd.)(REVIEW) (2007)
  • Author(s): Suzuki Y, Ohtani K, Wakamiya, N.
  • Total Pages: 85-101
  • Publisher: Research Spotlight
• [Remarks]
  • URL: http://www.asahikawa-med.ac.jp/dept/mc/microbio/microbiology.html
• [Patent(Industrial Property Rights)] 組換えマンナン結合タンパク質 (2009)
  • Inventor(s): 若宮伸隆
  • Industrial Property Rights Holder: 扶桑薬品工業株式会社
  • Industrial Property Number: PCT11/241035
  • Acquisition Date: 2009-08-11
  • Overseas
• [Patent(Industrial Property Rights)] コレクチン活性を有するhCL-K1ポリペプチド (2007)
  • Inventor(s): 若宮伸隆, その他4名
  • Industrial Property Rights Holder: 扶桑薬品工業株式会社
  • Industrial Property Number: PCTJP2007/057632
  • Filing Date: 2007-04-05
  • Overseas