Identification and functional analysis of novel bioactive peptides on feeding regulation

2009 Fiscal Year Final Research Report

• Project/Area Number: 19390254
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Endocrinology
• Research Institution: University of Miyazaki
• Principal Investigator: NAKAZATO Masamitsu University of Miyazaki, 医学部, 教授 (10180267)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Hideki 宮崎大学, 医学部, 講師 (10305097) ; TOSHINAI Koji 宮崎大学, 医学部, 特任助教 (80381101)
• Project Period (FY): 2007 – 2009
• Keywords: 摂食亢進 / 視床下部 / 新規生理活性ペプチド / NERP / オレキシン

Research Abstract

We have investigated the tissue localization and biological functions of novel bioactive peptides NERPs. Immunoreactive NERPs were present in lateral nucleus of the hypothalamus and colocalized with orexin. NERP-2, but not NERP-1, enhanced food intake in a manner dependent on the neuropeptides orexins. Bioactivity of NERPs required carboxy-terminal amidation. This study demonstrated that NERP-2 is a novel orexigenic bioactive peptide.

Research Products

• [Journal Article] Neuroendocrine regulatory peptide-1 and-2: novel bioactive peptides processed from VGF. (2009)
  • Author(s): Toshinai K Nakazato M.
  • Journal Title: Cell Mol Life Sci 66 Pages: 1939-1945
• [Journal Article] Plasma des-acyl ghrelin, but not plasma HMW adiponectin, is a useful cardiometabolic marker for predicting atherosclerosis in elderly hypertensive patients. (2009)
  • Author(s): Yano Y, Toshinai K, Inokuchi T, Kangawa K, Shimada K, Kario K, Nakazato M.
  • Journal Title: Atherosclerosis 204 Pages: 590-594
• [Journal Article] Ghrelin reverses experimental diabetic neuropathy in mice. (2009)
  • Author(s): Kyoraku I, Shiomi K, Kangawa K, Nakazato M.
  • Journal Title: Biochem Biophys Res Commun 389 Pages: 405-408
• [Journal Article] Effect on octanoic acid-rich formula on plasma ghrelin levels in cachectic with chronic respiratory diseases. (2009)
  • Author(s): Ashitani J, Matsumoto N, Nakazato M.
  • Journal Title: Nutritional J 16 Pages: 8-25
• [Journal Article] Ghrelin and its therapeutic potential for cachectic patients. (2009)
  • Author(s): Ashitani J, Matsumoto N, Nakazato M.
  • Journal Title: Peptides 30 Pages: 1951-1956
• [Journal Article] Effects of memantine on soluble Alphabeta (25-35)-induced changes in peptidergic and glial cells in Alzheimer's disease model rat brain regions. (2009)
  • Author(s): Arif M, Chikuma T, Ahmed MM, Nakazato M, Smith MA, Kato T.
  • Journal Title: Neuroscience 164 Pages: 1199-1209
• [Journal Article] Telmisartan attenuates fatty-acid-induced oxidative stress and NAD(P)H oxidase activity in pancreatic β-cells. (2009)
  • Author(s): Saitoh Y, Hongwei W, Ueno H, Mizuta M, Nakazato M.
  • Journal Title: Diabetes Metab 35 Pages: 392-397
• [Journal Article] Elevated alpha-defensin levels in plasma of patients with pulmonary sarcoidosis. (2008)
  • Author(s): Ashitani J, Matsumoto N, Nakazato M.
  • Journal Title: Respirology 12 Pages: 339-345
• [Journal Article] The role of PYY in feeding regulation. (2008)
  • Author(s): Ueno H, Yamaguchi H, Mizuta M, Nakazato M.
  • Journal Title: Regul Pept 145 Pages: 12-16
• [Journal Article] Ontogeny of a new enteric peptide, neuropeptide W (NPW), in the developing rat stomach. (2008)
  • Author(s): Mondal MS, Yamaguchi H, Date Y, Tsuruta T, Shimbara T, Toshinai K, Shimomura Y, Mori M, Nakazato M.
  • Journal Title: Regul Pept 145 Pages: 141-146
• [Journal Article] The vagal afferent pathway does not play a major role in the induction of satiety by intestinal fatty acid in rats. (2008)
  • Author(s): Ogawa N, Yamaguchi H, Shimbara T, Toshinai K, Kakutani M, Yonemori F, Nakazato M.
  • Journal Title: Neurosci Lett 433 Pages: 38-42
• [Journal Article] Pten controls lung morphogenesis, bronchioalveolar stem cells, and onset of lung adenocarcinomas. (2007)
  • Author(s): Yanagi S, Kishimoto H, Kawahara K, Sasaki T, Sasaki M, Nishio M, Yajima N, Hamada K, Horie Y, Kubo H, Mak TW, Nakano T, Nakazato M, Suzuki A.
  • Journal Title: J Clin Invest 117 Pages: 2929-2940
• [Journal Article] Peptidomic identification and biological validation of neuroendocrine regulatory peptide-1 and-2. (2007)
  • Author(s): Yamaguchi H, Sasaki K, Satomi Y, Shimbara T, Kageyama H, Mondal MS, Toshinai K, Date Y, Gonzalez LJ, Shioda S, Takao T, Nakazato M, Minamino N.
  • Journal Title: J Biol Chem 282 Pages: 26354-26360
• [Journal Article] Ghrelin stimulates growth hormone secretion and food intake in aged rats. (2007)
  • Author(s): Toshinai K, Mondal MS, Shimbara T, Yamaguchi H, Date Y, Kangawa K, Nakazato M.
  • Journal Title: Mech Ageing Dev 128 Pages: 182-186
• [Journal Article] Elevated levels of antimicrobial peptides in bronchoalveolar lavage fluid in patients with chronic eosinophilic pneumonia. (2007)
  • Author(s): Ashitani J, Matsumoto N, Nakazato M.
  • Journal Title: Respiration 74 Pages: 69-75
• [Journal Article] Acute incremental exercise decreases plasma ghrelin level in healthy men. (2007)
  • Author(s): Toshinai T, Kawagoe T, Shimbara T, Tobina T, Nishida Y, Mondal MS, Yamaguchi H, Date Y, Tanaka H, Nakazato M.
  • Journal Title: Horm Metab Res 39 Pages: 849-851
• [Journal Article] Plasma ghrelin concentrations in different clinical stages of diabetic complications and glycemic control in Japanese diabetics. (2007)
  • Author(s): Ueno H, Shiiya T, Mizuta M, Mondal MS, Nakazato M.
  • Journal Title: Endocrine J 54 Pages: 895-902
• [Journal Article] A hypothalamic peptide, neuroendocrine regulatory peptide-2, modulates the orexin system.
  • Author(s): oshinai K, Yamaguchi H, Kageyama H, Matsuo T, Koshinaka K, Sasaki K, Shioda S, Minamino N, Nakazato M.
  • Journal Title: Am J Physiol (in press)
• [Journal Article] Localization of neuroendocrine regulatory peptide-1 and-2 in human tissues.
  • Author(s): Matsuo T, Yamaguchi H, Kageyama H, Sakaki M, Shioda S, Minamino N, Nakazato M.
  • Journal Title: Regul Pept (in press)
• [Journal Article] Translational research of ghrelin.
  • Author(s): Ueno H, Shiiya T, Nakazato M.
  • Journal Title: Ann NY Acad Sci (in press)
• [Presentation] 胃で産生されるアナボリックペプチドグレリンの臨床応用の展開 (2009)
  • Author(s): 中里雅光
  • Organizer: Japan Digestive Disease week 2009
  • Year and Date: 20090000
• [Presentation] Recent progresses in neuropeptide research: from discovery to clinical application (2009)
  • Author(s): 中里雅光
  • Organizer: The 2009 Annual Meeting of Korean Endocrine Society
  • Year and Date: 20090000
• [Presentation] 新規視床下部ペプチドによる摂食調節
  • Author(s): 中里雅光
  • Organizer: 第50回日本糖尿病学会年次学術集会
• [Presentation] 摂食関連ペプチドのネットワーク
  • Author(s): 中里雅光
  • Organizer: 第80回日本内分泌学会総会
• [Presentation] ペプチドによる神経性の摂食調節機構
  • Author(s): 中里雅光
• [Presentation] グレリン治療の応用指針
  • Author(s): 中里雅光
  • Organizer: 第34回日本神経内分泌学会
• [Presentation] 消化管と脳を結ぶペプチド性摂食調節機構
  • Author(s): 中里雅光
  • Organizer: 07BMB第80回日本生化学会
• [Presentation] AVP分泌調節に関わる新規視床下部ペプチドNERPの発見と機能解析
  • Author(s): 中里雅光
  • Organizer: 第81回日本内分泌学会学術総会
• [Presentation] Ghrelin and novel peptides in the regulation of energy homeostasis
  • Author(s): 中里雅光
  • Organizer: 17th International Symposium on Regulatory peptides
• [Presentation] 加齢におけるアナボリックペプチドグレリンの意義とトランスレーショナルリサーチ
  • Author(s): 中里雅光
  • Organizer: 第9回日本抗加齢医学会総会
• [Presentation] Feeding Regulatory System Connecting
  • Author(s): 中里雅光
  • Organizer: The 82nd Annual Meeting of the Japan Endocrine Society
• [Presentation] Role of ghrelin in the central regulation of feeding
  • Author(s): 中里雅光
  • Organizer: XXXVI International Congress of Physiological Sciences
• [Presentation] Discovery of novel neuropeptides NERPs that suppress vasopressin secretion
  • Author(s): 中里雅光
  • Organizer: VIIIth World Congress on Neurohypophysial Hormones
• [Presentation] Translational Research of Gastrointestinal Peptides
  • Author(s): 中里雅光
  • Organizer: atellite Symposium of the 9th International Symposium on VIP, PACAP and Related Peptides
• [Presentation] ペプチドによる中枢と末梢を結ぶ摂食調節機構
  • Author(s): 中里雅光
  • Organizer: 第82回日本生化学会大会
• [Presentation] 摂食調節ペプチドのトランスレーショナルリサーチ
  • Author(s): 中里雅光
  • Organizer: 第30回日本肥満学会
• [Presentation] 摂食調節物質とトランスレーショナルリサーチの実際
  • Author(s): 中里雅光
  • Organizer: 第44回糖尿病学の進歩
• [Book] New Frontiers in Neuroscience, Feeding regulation in the brain: Involvement of neuropeptide W (2009)
  • Author(s): Takenoha F, Kageyama H, Date Y, Nakazato M, Shioda S
  • Total Pages: 210
  • Publisher: Springer
• [Book] 実験医学増刊肥満・糖尿病の病態を解明するエネルギー代謝の最前線-ミトコンドリア・脂肪細胞の機能理解から, 臓器間神経ネットワークによる代謝調節まで, 摂食調節2. 視床下部における摂食調節物質の新知見 (2009)
  • Author(s): 中里雅光
  • Total Pages: 213
  • Publisher: 羊土社
• [Book] New frontiers in Lifestyle-Related Diseases (2007)
  • Author(s): Nakazato M, Ueno H
  • Total Pages: 159
  • Publisher: Springer
• [Book] 糖尿病ナビゲータ
  • Author(s): 中里雅光
  • Publisher: メディカルレビュー社(in pres)
• [Patent(Industrial Property Rights)] グレリン及びその誘導体又はghsr1aアンタゴニストを有効成分とする慢性呼吸器感染治療 (2008)
  • Inventor(s): 中里雅光, 芦谷淳一, 小玉剛士, 寒川賢治, 林友二郎
  • Industrial Property Rights Holder: 中里雅光,芦谷淳一,小玉剛士, 寒川賢治,林友二郎
  • Industrial Property Number: 特願・2008-88324
  • Acquisition Date: 2008-03-28
• [Patent(Industrial Property Rights)] 脳血管疾患の診断法 (2007)
  • Inventor(s): 塩見一剛, 中里雅光, 浜窪隆雄, 児玉龍彦, 相良三奈
  • Industrial Property Rights Holder: 塩見一剛,中里雅光,浜窪隆雄, 児玉龍彦,相良三奈
  • Industrial Property Number: 特許・2007-184373
  • Acquisition Date: 2007-07-13
• [Patent(Industrial Property Rights)] 鼻腔内投薬装置 (2007)
  • Inventor(s): 藤原光輝, 中里雅光
  • Industrial Property Rights Holder: 藤原光輝,中里雅光
  • Industrial Property Number: 特願・2007-331207
  • Acquisition Date: 2007-12-22
• [Patent(Industrial Property Rights)] 糖尿病性神経障害治療薬 (2007)
  • Inventor(s): 中里雅光, 京楽格, 塩見一剛, 林友二郎, 寒川賢治
  • Industrial Property Rights Holder: 中里雅光,京楽格,塩見一剛, 林友二郎,寒川賢治
  • Industrial Property Number: 特願・2007-122897
  • Acquisition Date: 2007-05-07