2009 Fiscal Year Final Research Report
Molecular regulation of intracellular calcium signaling in the region-specific cardiac development
| Project/Area Number |
19390288
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA Keiko 慶應義塾大学, 医学部, 共同研究員 (50286522)
YAMAGISHI Chihiro 慶應義塾大学, 医学部, 共同研究員 (10296618)
MAKINO Shinji 慶應義塾大学, 医学部, 准教授 (20306707)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 小児循環器学 |
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| Research Abstract |
Intracellular calcium signaling is known to play essential roles in cardiac physiology and hypertrophy, however, its function in cardiac development remains to be elucidated. Inositol 1,4,5-trisphosphate receptors (IP_3R type1, 2 and 3) are intracellular calcium release channels on the endo/sarcoplasmic reticulum. IP_3R1, 2 double knockout mouse (DKO) embryos and IP_3R1, 3 DKO embryos died around E10.5 with signs of heart failure while each single knockout mouse showed normal cardiogenesis. In the IP_3R1, 2 DKO embryos, the atrioventricular cushion development was impaired involving the NFATc signal, whereas the outflow tract development, implicated in the second heart field, was affected in the IP_3R1, 3 DKO embryos. This research project revealed that intracellular calcium signaling redundantly mediated by three subtypes of IP_3R may play a role in development of each specific module of cardiovascular development.
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