2009 Fiscal Year Final Research Report
Development of Novel Therapy by miRNA Targeting for Gastroenterological Cancer
Project/Area Number |
19390338
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General surgery
|
Research Institution | Kumamoto University |
Principal Investigator |
BABA Hideo Kumamoto University, 大学院・生命科学研究部, 教授 (20240905)
|
Co-Investigator(Kenkyū-buntansha) |
KAMOHARA Hidenobu 熊本大学, 医学部附属病院, 講師 (90398222)
|
Project Period (FY) |
2007 – 2009
|
Keywords | 実験外科学 / 消化器癌 / 分子生物学 / microRNA |
Research Abstract |
Among 20 paired samples, 18 cancer tissues overexpressed miR-21 compared with matched normal epitheliums. Especially, patients with lymph node metastasis or venous invasion showed high expression of miR-21 significantly. All 7 ESCC cell lines also overexpressed miR-21 and anti-miR-21 transfected ESCC cells showed significant reduction in cellular proliferation and invasion. The PDCD4 protein in ESCC cells have inverse correlation with miR-21 expression significantly. Anti-miR-21 transfected ESCC cells increased PDCD4 protein without differences of PDCD4-mRNA and increased a luciferase-reporter activity containing the PDCD4-3'-UTR region. Among 120 ESCC samples, immunohistchmeistry study revealed that patients expressed PDCD4 protein prolonged survival compared with negative. Our results suggest miR-21 plays a pivotal role in progression of ESCC, and it might serve as a novel therapeutic target.
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Research Products
(120 results)