Inhibition of metastasis and tetraspanin expression by the human monoclonal antibody directed to CD151 and RNA interference

2008 Fiscal Year Final Research Report

• Project/Area Number: 19390369
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Tazuke Kofukai Medical Research Institute
• Principal Investigator: MIYAKE Masayuki Tazuke Kofukai Medical Research Institute, 医学研究所・第1研究部, 研究員 (90250076)
• Co-Investigator(Kenkyū-buntansha): OMASA Mitsugu 財団法人田附興風会, 医学研究所・第1研究部, 研究員 (80379049)
• Co-Investigator(Renkei-kenkyūsha): MITSUDOMI Tetsuya 愛知県がんセンター, 研究所, 研究員 (70209807)
• Project Period (FY): 2007 – 2008
• Keywords: 転移促進遺伝子 / CD151 / MRP1 / CD9 / KAI1 / CD82 / テトラスパニン / 癌転移

Research Abstract

siRNAで、CD151を中心としたインテグリン・テトラスパニン複合構成体を抑制、崩壊させることに成功した。さらに、CD151のノックアウトを行った後にMRP-1/CD9、KAI1/CD82の遺伝子治療によるカクテル療法を細胞株で行い、in vitroレベルでは、成功した。しかし、これらの細胞株を、ヌードマウスに生着させ、in vivoで数度のカクテル療法を行ったが、失敗に終わった。スキッドマウスでは、成功したので、この原因は、遺伝子導入におけるウイルスベクターの使用によるウイルス抗原に対する抗体の産生などによると考えた

Research Products

• [Journal Article] Stepwise examination for differential diagnosis of primary lung cancer and breast cancer relapse presenting as a solitary pulmonary nodule in patients after mastectomy (2008)
  • Author(s): Okasaka, T., Usami, N., Mitsudomi, T., Yatabe, Y., Matsuo, K., and Yokoi, K
  • Journal Title: J Surg Oncol. 1 98(7) Pages: 510-514
  • Peer Reviewed
• [Journal Article] Predictors of survival in patients with bone metastasis of lung cancer (2008)
  • Author(s): Sugiura, H., Yamada, K., Sugiura, T., Hida, T., and Mitsudomi, T
  • Journal Title: Clin Orthop Relat Res 466(3) Pages: 729-36
  • Peer Reviewed
• [Journal Article] Adenoviral Transduction of MRP-1/CD9 and KAI1/CD82 Inhibits Lymph Node Metastasis in Orthotopic Lung Cancer Model (2007)
  • Author(s): Takeda, T., Hattori, N., Tokuhara, T., Nishimura, Y., Yokoyama, M., and Miyake, M
  • Journal Title: Cancer Res 67 Pages: 1744-1749
  • Peer Reviewed
• [Journal Article] Clinicopathological significance of aminopeptidase N/CD13 expression in human gastric carcinoma (2007)
  • Author(s): Kawamura, J., Shimada, Y., Kitaichi, H., Komoto, I., Hashimoto, Y., Kaganoi, J., Miyake, M., Yamasaki, S., Kondo, K., Imamura, M
  • Journal Title: Hepatogastroenterology 54(73) Pages: 36-40
  • Peer Reviewed
• [Journal Article] Lineage-specific dependency of lung adenocarcinomas on the lung development regulator (2007)
  • Author(s): Tanaka, H., Yanagisawa, K., Shinjo, K., Taguchi, A., Maeno, K., Tomida, S., Shimada, Y., Osada, H., Kosaka, T., Matsubara, H., Mitsudomi, T., Sekido, Y., Tanimoto, M., Yatabe, Y., and Takahashi, T.
  • Journal Title: TTF-1Cancer Res. 1 67(13) Pages: 6007-11
  • Peer Reviewed
• [Journal Article] A 25-signal proteomic signature and outcome for patients with resected non-small-cell lung cance (2007)
  • Author(s): Yanagisawa, K., Tomida, S., Shimada, Y., Yatabe, Y., Mitsudomi, T., and Takahashi, T
  • Journal Title: J Natl Cancer Inst. 6 99(11) Pages: 858-67
  • Peer Reviewed
• [Journal Article] Controversy about small peripheral lung adenocarcinomas: how should we manage them? (2007)
  • Author(s): Fukui, T., Sakakura, N., Mori, S., Hatooka, S., Shinoda, M., Yatabe, Y., and Mitsudomi, T
  • Journal Title: J Thorac Oncol 2(6) Pages: 546-52
  • Peer Reviewed
• [Journal Article] Impact of one-carbon metabolism-related gene polymorphisms on risk of lung cancer in Japan: a case control study (2007)
  • Author(s): Suzuki, T., Matsuo, K., Hiraki, A., Saito, T., Sato, S., Yatabe, Y., Mitsudomi, T., Hida, T., Ueda, R., Tajima, K
  • Journal Title: Carcinogenesis 28(8) Pages: 1718-25
  • Peer Reviewed
• [Presentation] Gene Transduction Of RhoA And ROCK-Dominant Negative Form Suppresses Lung Metastasis Of Melanoma (2008)
  • Author(s): Takeda,T. Hattori,N. Tokuhara,T. Takeda,S.M. Miyake
  • Organizer: The 2008 International Conference of American Thoracic Society (ATS)
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20080516-21
• [Presentation] Suppression of metastasis by human-type monoclonal antibody recognizing aminopeptidase N (2007)
  • Author(s): Tokuhara, T., Hirai, T., Miyake, M
  • Organizer: The Annual Meeting 2007 of American Association for Cancer Research
  • Place of Presentation: Los Angeles, California, U.S.A.
  • Year and Date: 20070414-18