2009 Fiscal Year Final Research Report
AAV vector-mediated cell and gene therapy for neuronal diseases
| Project/Area Number |
19390383
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
OKADA Takashi National Center of Neurology and Psychiatry, 神経研究所・遺伝子疾患治療研究部, 室長 (00326828)
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| Project Period (FY) |
2007 – 2009
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| Keywords | AAVベクター / 遺伝子治療 / 神経疾患 |
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| Research Abstract |
To realize novel protein supplementation therapy for neuronal diseases, we have developed systems for AAV vector production and vector-producing cells. By using effective ion-exchange adsorbers, improved purification of vector particles was investigated. In addition to the step-gradient ultracentrifugation procedure, dual ion-exchange procedures enhanced removal of the empty capsids to realize highly-purified vector production at hand. As an application for the AAV-mediated protein supplementation therapy, therapeutic effects of anti-inflammatory treatment on the mice stroke model were successfully demonstrated. Furthermore, vector-producing mesenchymal stem cells to target glioma were developed to target seat of disease with improved anti-tumor effects in vivo.
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[Journal Article] Systemic delivery of IL-10 by an AAV vector prevents vascular remodeling and end-organ damage in stroke-prone spontaneously hypertensive rat.2009
Author(s)
Nomoto T., Okada T., Shimazaki K., Yoshioka T., Nonaka-Sarukawa M., Ito T., Takeuchi K., Katsura K.I., Mizukami H., Kume A., Ookawara S., Ikeda U., Katayama Y., Ozawa K.
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Journal Title
Gene Ther 16
Pages: 383-91
Peer Reviewed
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[Journal Article] Interleukin-10 Expression Mediated by an Adeno-Associated Virus Vector Prevents Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats.2007
Author(s)
Ito T., Okada T., Miyashita H., Nomoto T., Nonaka-Sarukawa M., Uchibori R., Maeda Y., Urabe M., Mizukami H., Kume A., Takahashi M., Ikeda U., Shimada K., Ozawa K.
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Journal Title
Circ Res 101
Pages: 734-741
Peer Reviewed
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