Identification and characterization of autoantibody against granulocyte-macrophage colony-stimulating factor in healthy individuals.

2010 Fiscal Year Final Research Report

• Project/Area Number: 19390403
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: The University of Tokyo
• Principal Investigator: YAMADA Yoshitsugu The University of Tokyo, 医学部附属病院, 教授 (30166748)
• Co-Investigator(Kenkyū-buntansha): CHANG Kyungho 東京大学, 医学部附属病院, 講師 (50302708) ; KITAMURA Takayuki 東京大学, 医学部附属病院, 助教 (50302609) ; UCHIDA Kanji 東京大学, 医学部附属病院, 助教 (60302709)
• Project Period (FY): 2007 – 2010
• Keywords: 周術期管理学 / GM-CSF / 自己抗体 / 好中球機能 / 急性肺傷害 / SIRS / サイトカイン

Research Abstract

Autoantibodies against granulocyte-macrophage colony-stimulating factor (GM-CSF) were reported by Nakata et al. in 1999. We detected low level of anti-GM-CSF autoantibody in serum from healthy individuals. It has neutralizing capacity and forms circulating immune complex in the serum, and may modulate myeloid cell immune functions. Anti-GM-CSF autoantibody may have a buffering effect to modulate GM-CSF bioactivity in vivo.

Research Products

• [Journal Article] Granulocyte/macrophage colony-stimulating factor autoantibodies and myeloid cell immune functions in healthy persons. (2010)
  • Author(s): Uchida K, Carey B, Suzuki T, Nakata K, Trapnell B. Response
  • Journal Title: Blood. 14 ; 115(2) Pages: 431-3
• [Journal Article] Patient-derived granulocyte/macrophage colony-stimulating factor autoantibodies reproduce pulmonary alveolar proteinosis in nonhuman primates. (2010)
  • Author(s): Sakagami T, Beck D, Uchida K, Suzuki T, Carey BC, Nakata K, Keller G, Wood RE, Wert SE, Ikegami M, Whitsett JA, Luisetti M, Davies S, Krischer JP, Brody A, Ryckman F, Trapnell BC
  • Journal Title: Am J Respir Crit Care Med. 1 ; 182(1) Pages: 49-61
  • Peer Reviewed
• [Journal Article] High avidity cytokine autoantibodies in health and disease : pathogenesis and mechanisms. (2010)
  • Author(s): Watanabe M, Uchida K, Nakagaki K, Trapnell BC, Nakata K
  • Journal Title: Cytokine Growth Factor Rev. 21(4) Pages: 263-73
  • Peer Reviewed
• [Journal Article] Granulocyte/macrophage-colony-stimulating factor autoantibodies and myeloid cell immune functions in healthy subjects. (2009)
  • Author(s): Uchida K, Nakata K, Suzuki T, Luisetti M, Watanabe M, Koch DE, Stevens CA, Beck DC, Denson LA, Carey BC, Keicho N, Krischer JP, Yamada Y, Trapnell BC
  • Journal Title: Blood. 2 ; 113(11) Pages: 2547-56
  • Peer Reviewed
• [Journal Article] Granulocyte-macrophage colony-stimulating factor autoantibodies in murine ileitis and progressive ileal Crohn's disease. (2009)
  • Author(s): Han X, Uchida K, Jurickova I, Koch D, Willson T, Samson C, Bonkowski E, Trauernicht A, Kim MO, Tomer G, Dubinsky M, Plevy S, Kugathsan S, Trapnell BC, Denson LA
  • Journal Title: Gastroenterology. 136(4) Pages: 1261-71
  • Peer Reviewed
• [Journal Article] Pulmonary alveolar proteinosis, a primary immunodeficiency of impaired GM-CSF stimulation of macrophages. (2009)
  • Author(s): Trapnell BC, Carey BC, Uchida K, Suzuki T
  • Journal Title: Curr Opin Immunol. 21(5) Pages: 514-21
  • Peer Reviewed
• [Journal Article] Human GM-CSF autoantibodies and reproduction of pulmonary alveolar proteinosis. (2009)
  • Author(s): Sakagami T, Uchida K, Suzuki T, Carey BC, Wood RE, Wert SE, Whitsett JA, Trapnell BC, Luisetti M
  • Journal Title: N Engl J Med. 31 ; 361(27) Pages: 2679-81
  • Peer Reviewed
• [Presentation] Meet the Professor Seminar ; MP401 : GM-CSF AUTOANTIBODY ; DISCOVERY, FUNCTIONS AND FUTURE APPLICATIONS. (2010)
  • Author(s): Uchida K, Nakata K
  • Organizer: American Thoracic Society Annual International Conference.
  • Place of Presentation: New Orleans, Louisiana, USA
  • Year and Date: 2010-05-16
• [Presentation] 食道癌手術患者の侵襲に応じた好中球機能低下は術後早期回復に影響する (2009)
  • Author(s): 内田寛治、張京浩、山田芳嗣
  • Organizer: 日本麻酔科学会学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2009-08-17
• [Presentation] Reduced Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Bioactivity with Surgical Stress Associated with Early Postoperative Complications. (2009)
  • Author(s): 内田寛治, 張京浩, 山田芳嗣
  • Organizer: American Thoracic Society International Conference.
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-05-19
• [Presentation] Serologic Diagnosis of Pulmonary Alveolar Proteinosis (PAP). (2009)
  • Author(s): 内田寛治, 山田芳嗣
  • Organizer: American Thoracic Society International Conference.
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-05-18
• [Presentation] ワークショップ3「基礎:炎症細胞の分離と機能的解析」「好中球」 (2008)
  • Author(s): 内田寛治
  • Organizer: 日本アレルギー学会秋季学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2008-11-27
• [Presentation] フローサイトメトリー法による簡易好中球活性化指標測定法の開発 (2008)
  • Author(s): 内田寛治、張京浩、山田芳嗣
  • Organizer: 日本麻酔科学会学術集会
  • Place of Presentation: 横浜市、神奈川県
  • Year and Date: 2008-06-12
• [Presentation] A Standardized, Serum-Based Approach For Diagnosing PAP. (2008)
  • Author(s): 内田寛治
  • Organizer: American Thoracic Society International Conference
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-05-21
• [Book] Pulmonary Alveolar Proteinosis in Interstitial Lung Diseases. (2009)
  • Author(s): Uchida K, Trapnell BC
  • Total Pages: 208-224
  • Publisher: European Respiratory Monograph.
• [Remarks] ホームページ等