Effects of Notch Signaling on the Pulpal Cell Differentiation and the Horizon of Pulpal Regeneration by Regulation of Notch Signaling

2009 Fiscal Year Self-evaluation Report

• Project/Area Number: 19390481
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KAWASHIMA Nobuyuki Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 助教 (60272605)
• Project Period (FY): 2007 – 2009
• Keywords: 歯髄細胞 / 象牙芽細胞 / Notchシグナル / 歯髄細胞分化 / 歯髄再生

Research Abstract

健康な歯で物を噛むことが出来るのはQOLを高める上で大変重要である。そのため我々歯科医はいかに歯を保存できるよう常に配慮して診療を行っている。ところで、歯の内部に存在する神経と血管に富んだ歯髄組織は、歯が口腔内において健全に機能する上で大変重要であるが、炎症(歯髄炎)を起した歯髄組織は除去せざるを得ない。このような現状を鑑み、本研究においては、歯髄細胞の分化メカニズムの一端を明らかにし、それを元に臨床的な歯髄再生のシナリオを描くのが本研究の目的である。歯髄細胞の分化メカニズムを明らかにする上で、様々な組織・細胞の分化に深く関わっているNotchシグナルを切り口とする点が本研究の特色である。下記項目について研究を行った。
(1)Notchおよびその下流のシグナルの発現動態の解明(歯胚および成熟歯髄)
(2)Notchおよびその下流のシグナルの歯髄細胞および歯髄幹細胞における機能的な解析
(3)Notchおよびその下流のシグナルを応用し、歯髄細胞を分化誘導するための臨床的検討

Research Products

• [Journal Article] Expression of Notch-Signalling-related Genes in Normal and Differentiating Rat Dental Pulp Cells (2009)
  • Author(s): Sun H, Kawashima N, Xu J, Takahashi S, Suda H
  • Journal Title: AEJ (in press)
  • Peer Reviewed
• [Journal Article] Kawashima N, Wadachi R, Suda H, Yeng T, Parashos P. (2009)
  • Author(s): Root Canal Medicaments
  • Journal Title: Int Dent J 59(1) Pages: 5-11
  • Peer Reviewed
• [Journal Article] T Cell Response Mediated by Myeloid Cell-Derived IL-12 Is Responsible for Porphyromonas gingivalis-Induced Periodontitis in IL-10-Deficient Mice (2008)
  • Author(s): Sasaki H, Suzuki N, Kent R, Kawashima N, Takeda J, Stashenko P
  • Journal Title: The Journal of Immunology 180 Pages: 6193-6198
  • Peer Reviewed
• [Journal Article] Amelogenin positive cells scattered in the interstitial component of odontogenic fibromas (2008)
  • Author(s): Kabasawa Y, Nagumo K, Takeda Y, Kawashima N, Okada N, Omura K, Yamaguchi A, Katsube K
  • Journal Title: J. Clin 61(Pathol., Jul) Pages: 851-855
  • Peer Reviewed
• [Journal Article] Kinetics of RANKL, RANK and OPG expressions in experimentally-induced rat periapical lesions (2007)
  • Author(s): Kawashima N., Suzuki N., Yang G., Ohi C., Okuhara S., Kawanishi H., Suda H.
  • Journal Title: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 103 Pages: 707-711
  • Peer Reviewed
• [Journal Article] Kinetic study of immunohistochemical colocalization of antigen presenting cells and nerve fibers in rat periapical lesions (2007)
  • Author(s): Yang G, Kawashima N, Kaneko T, Suzuki N, Okiji T, Suda H J.
  • Journal Title: Endodon 33(2) Pages: 132-136
  • Peer Reviewed
• [Journal Article] Differentiation of an ameloblast-lineage cell line (ALC) is induced by Sonic hedgehog signaling (2007)
  • Author(s): Takahashi S, Kawashima N, Sakamoto K, Nakata A, Kameda T, Sugiyama T, Katsube K, Suda H, BBRC
  • Journal Title: 353 Pages: 405-411
  • Peer Reviewed
• [Presentation] Induction of IL17 synthesis in the experimentally-induced murine periapical lesions (2010)
  • Author(s): Kawashima N., et. al.
  • Organizer: Korean Academy of Endodontics, BEXCO Busan, Korea
  • Year and Date: 2010-03-27
• [Presentation] Expression of Sp7 in the rat dental pulp (2009)
  • Author(s): Kawashima N., et. al.
  • Organizer: Korean Academy of Conservative Dentistry
  • Place of Presentation: Ramada Plaza, Jeju, Korea
  • Year and Date: 2009-11-13
• [Presentation] Notch Signaling in the Dental Pulp (2008)
  • Author(s): Kawashima N., et. al.
  • Organizer: IADR, Metro Toronto Convention Centre
  • Place of Presentation: ON, Canada
  • Year and Date: 2008-07-02
• [Book] Essential Endodontology(Orstavik D and Pitt Ford TR editors, Pathological aspects of pulpal and periapical inflammations) (2007)
  • Author(s): Kawashima N, Suda H
  • Total Pages: 44-80
  • Publisher: Blackwell Publishing
• [Patent(Industrial Property Rights)] 歯周病又は根尖性歯周炎の予防又は治療薬 (2009)
  • Inventor(s): 川島伸之, 鈴木規元, 他
  • Industrial Property Rights Holder: 川島伸之, 鈴木規元, 他
  • Industrial Property Number: 2009-218590
  • Filing Date: 2009-09-24