An imaging and drug delivary system for treatment of oral cancer by using ultrasound and nano-bubbles

2009 Fiscal Year Final Research Report

• Project/Area Number: 19390507
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Tohoku University
• Principal Investigator: MORI Shiro Tohoku University, 病院, 講師 (80230069)
• Co-Investigator(Renkei-kenkyūsha): KODAMA Tetsuya 東北大学, 医工学研究科, 教授 (40271986) ; ONO Masao 東北大学, 医学系研究科, 教授 (20302218)
• Project Period (FY): 2007 – 2009
• Keywords: 口腔癌 / ナノバブル / 超音波 / 画像診断 / 分子導入 / 動物モデル / 腫瘍血管 / 画像構築

Research Abstract

In the present study, we try to develop a three-dementional imaging system and drug delivery system by using nanobubbles and ultrasound for diagnosis and treatment of oral cancer. As a result, we could make three-dementional ultrasound imaging of neovascularization in small tumor, which could not be detectable by using conventional imaging system such as CT or MRI. Moreover, when the treatment experiment of tumor by the drug delivery system that used the nanobubbles and ultrasound was done, the therapeutic gain by this method was able to be confirmed.

Research Products

• [Journal Article] Delivery of Na/I symporter gene into skeletal muscle by using nanobubbles and ultrasound : Visualization of gene expression by positron emission tomography (2010)
  • Author(s): Watanabe Y, Horie S, Funaki Y, Kikuchi Y, Yamazaki H, Ishii K, Mori S, Vassaux G, Kodama T
  • Journal Title: Journal of Nuclear Medicine (in press)
  • Peer Reviewed
• [Journal Article] Morphological study on Acoustic liposome using transmission electron microscopy (2010)
  • Author(s): Kodama T, Tomita N, Horie S, Sax N, Iwasaki H, Suzuki R, Maruyama K, Mori S, Fukumoto M
  • Journal Title: J Electron Microsc (In Press)
  • Peer Reviewed
• [Journal Article] Evaluating the Role of Rheumatoid Factors for the Development of Rheumatoid Arthritis in a Mouse Model with a Newly Established ELISA System (2010)
  • Author(s): Tanaka Y, Komori H, Mori S, Soga Y, Tsubaki T, Terada M, Miyazaki T, Fujino T, Nakamura S, Kanno H, Sawasaki T, Endo Y, Nose M
  • Journal Title: Tohoku J Exp Med 220(3) Pages: 199-206
  • Peer Reviewed
• [Journal Article] Periodontal Gene Transfer by Ultrasound and Nano-/Microbubbles (2009)
  • Author(s): R. Chen, Chiba M, Mori S, Fukumoto M, Kodama T
  • Journal Title: J Dent Res 88(11) Pages: 1008-1013
  • Peer Reviewed
• [Journal Article] Herpes simplex virus thymidine kinase-mediated suicide gene therapy using nano/microbubbles and ultrasound (2008)
  • Author(s): Aoi A, Watanabe Y, Mori S, Takahashi M, Vassaux G, Kodama T
  • Journal Title: Ultrasound Med Biol 34(3) Pages: 425-34
  • Peer Reviewed
• [Journal Article] A novel recombinant congenic mouse strain developing arthritis with enthesopathy (2008)
  • Author(s): Mori S, Tanda N, Ito MR, Oishi H, Tsubaki T, Komori H, Zhang MC, Ono M, Nishimura M, Nose M
  • Journal Title: Pathol Int 58(7) Pages: 407-414
  • Peer Reviewed
• [Journal Article] Low-intensity ultrasound and microbubbles enhance the antitumor effect of cisplatin (2008)
  • Author(s): Watanabe Y, Aoi A, Horie S, Tomita N, Mori S, Morikawa H, Matsumura Y, Vassaux G, Kodama T
  • Journal Title: Cancer Sci 99(12) Pages: 2525-2531
  • Peer Reviewed
• [Journal Article] Loss of heterozygosity of the PTH/PTHrP type 1 receptor in oral squamous cell carcinoma (2008)
  • Author(s): Miyashita H, Mori S, Fukumoto Y, Sato A, Fukumoto M, Kawamura H
  • Journal Title: Molecular Medicine Reports 1 Pages: 821-825
  • Peer Reviewed
• [Journal Article] Autosomal loci associated with a sex-related difference in the development of autoimmune phenotypes in a lupus model (2007)
  • Author(s): Misu N, Zhang MC, Mori S, Miyazaki T, Furukawa H, Sasaki T, Nose M, Ono M
  • Journal Title: Eur J Immunol 37(10) Pages: 2787-2796
  • Peer Reviewed
• [Journal Article] A non-MHC locus determines tissue-specificity in the pathogenic process underlying synovial proliferation in a mouse arthropathy model (2007)
  • Author(s): Zhang MC, Mori S, Date F, Furukawa H, Ono M
  • Journal Title: Ann Rheum Dis 66 Pages: 242-245
  • Peer Reviewed
• [Presentation] Targeted gene delivery using nanobubble and ultrasound (2010)
  • Author(s): Horie S, Watanabe Y, Chen R, Mori S, Matsumura Y, Kodama T
  • Organizer: The 5th International Symposium on Medical, Bio -and Nano-Electronics
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20100224-20100225
• [Presentation] PET imaging of Na/I symporter gene expression induced by nanobubbles and ultrasound (2010)
  • Author(s): Watanabe Y, Horie S, Funaki Y, Kikuchi Y, Yamazaki H, Ishii K, Mori S, Kodama T
  • Organizer: The 5th International Symposium on Medical, Bio- and Nano-Electronics
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20100224-20100225
• [Presentation] Observation for angiogenesis of liver metastases in preclinical models (2010)
  • Author(s): Chen R, YWatanabe Y, Li L, Horie S, Mori S, Fukumoto M, Kodama T.
  • Organizer: The 5th International Symposium on Medical, Bio-and Nano-Electronics
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20100224-20100225
• [Presentation] Four-dimensional high-frequency ultrasound imaging system for early detection of lymph node micro-metastasis (2010)
  • Author(s): Li L, Horie S, CHEN R, Watanabe Y, Baba T, Sax N, Sakamoto M, Mori S, Takahashi S, Kodama T.
  • Organizer: The 5th International Symposium on Medical, Bio- and Nano-Electronics
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20100224-20100225
• [Presentation] TEM observation and analysis of echogenic nano-bubbles (2010)
  • Author(s): Sax N, Horie S, Li L, Chen R, Watanabe Y, Mori S, Kodama T.
  • Organizer: The 5th International Symposium on Medical, Bio- and Nano-Electronics
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20100224-20100225
• [Presentation] Volumetric and angiogenetic imaging system by using nanobubbles and high-frequency ultrasound for evaluation of the antitumor effect by cisplatin (2010)
  • Author(s): Yagishita Y, Takata Y, Ohki K, Miyashita H, Morikawa H, Sakamoto M, Mori S, Kawamura H, Kodama T.
  • Organizer: The 5th International Symposium on Medical, Bio-and Nano-Electronics
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20100224-20100225
• [Presentation] Ultrasound molecular imaging of lymph node metastasis with nano/microbubbles (2010)
  • Author(s): Li L, Chen R, Horie S, Watanabe Y, Baba T, Sax N, Sakamoto M, Mori S, Takahashi S, Kodama T.
  • Organizer: International Symposium of AIDS and Tuberculosis (ISAT2010)
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20100113-20100114
• [Presentation] Physical characterization of acoustic liposomes (2010)
  • Author(s): Sax N, Horie S, Li L, Chen R, Watanabe Y, Mori S, Kodama T.
  • Organizer: International Symposium of AIDS and Tuberculosis (ISAT2010)
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20100113-20100114
• [Presentation] Three-dimentional high-frequency ultrasound imaging for early diagnosis of lymph node metastasis combined with microbubbles (2009)
  • Author(s): Li L, Horie S, Chen R, Watanabe Y, Sakamoto M, Mori S, Takahashi S, Kodama T.
  • Organizer: The 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering
  • Place of Presentation: Engineering Auditorium, National University of Singapore, Singapore
  • Year and Date: 20091221-20091222
• [Presentation] ナノバブルと高周波超音波画像診断装置の遠隔地口腔診断への応用 (2009)
  • Author(s): 森士朗, 他
  • Organizer: 第54回日本口腔外科学総会
  • Place of Presentation: 札幌
  • Year and Date: 20091009-20091011
• [Presentation] Novel approach to early diagnosis of lymph node metastasis with ultrasonic molecular imaging (2009)
  • Author(s): Li L, Horie S, Chen R, Watanabe Y, Mori S, Ono M, Sakamoto M, Takahashi S, Kodama T
  • Organizer: 第68回本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20091001-20091003
• [Presentation] ナノバブルと超音波を用いた口腔癌リアルタイム画像診断システムの開発に向けての検討 (2009)
  • Author(s): 森士朗, 他
  • Organizer: 第33回日本頭頸部癌学会
  • Place of Presentation: 札幌
  • Year and Date: 20090611-20090612
• [Presentation] Development of diagnostic imaging system for regional lymph node micrometastasis with high-frequency ultrasound (2008)
  • Author(s): Tomita N, Horie S, Oosawa F, Rui C, Watanabe Y, Morikawa H, Ohki K, Fukumoto M, Mori S, Kodama T.
  • Organizer: 2008 IEEE International Ultrasonics Symposium (IUS)
  • Place of Presentation: Beijing International Convention Center (BICC), Beijing, China
  • Year and Date: 20081102-20081105
• [Presentation] Contrast-Enhanced High-Frequency Ultrasound Imaging of Liver Metastases in Preclinical Models (2008)
  • Author(s): Chen R, Tomita N, Baba T, Oosawa F, Watanabe Y, Horie S, Mori S, Fukumoto M, Kodama T.
  • Organizer: 2008 IEEE International Ultrasonics Symposium (IUS)
  • Place of Presentation: Beijing International Convention Center (BICC), Beijing, China
  • Year and Date: 20081102-20081105
• [Presentation] Local gene delivery system of nano/microbubbles-enhanced ultrasound aimed for treatment of gingival tumor (2008)
  • Author(s): Chen R, Chiba M, Watanabe Y, Horie S, Tomita N, Fukumoto M, Mori S, Kodama T.
  • Organizer: 2008 International Conference on Frontiers of Dental and Craniofacial Research
  • Place of Presentation: Beijing International Convention Center (BICC), Beijing, China
  • Year and Date: 20081101-20081103
• [Presentation] ナノバブルと超音波を用いた口腔癌の治療のための新しい画像診断・分子導入システム (2008)
  • Author(s): 森士朗, 他
  • Organizer: 第53回日本口腔外科学総会
  • Place of Presentation: 徳島
  • Year and Date: 20081020-20081021
• [Presentation] Bladder cancer therapy using nanobubbles and two different intensities of ultrasound (2008)
  • Author(s): Horie S, Watanabe Y, Chen R, Tomita N, Oosawa F, Fujisawa S, Ono M, Fukumoto M, Mori S, Matsumura Y, Kodama T.
  • Organizer: The 2008 Nanomedicine Conference
  • Place of Presentation: Hotel Eden Roc, Sant Feliu de Guixols, Spain
  • Year and Date: 20080919-20080924
• [Presentation] 口腔癌の診断・治療のためのナノバブルと超音波を用いた画像診断・分子導入システムの開発 (2008)
  • Author(s): 森士朗, 他
  • Organizer: 第32回日本頭頸部癌学会
  • Place of Presentation: 東京
  • Year and Date: 20080612-20080613
• [Presentation] Evaluation of cisplatin-induced antitumor effect with contrast-enhanced high-frequency ultrasound (2008)
  • Author(s): Tomita N, Horie S, Watanabe Y, Ooswa F, Sakuma M, Chen R, Ohki K, Morikawa H, Mori S, Ono M, Kodama T
  • Organizer: 11th International Symposium on Advanced Biomedical Ultrasound
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2008-03-07
• [Presentation] High frequency ultrasound for analysis of antitumor effects (2007)
  • Author(s): Horie S, Tomita N, Watanabe Y, Osawa F, Sakuma M, Cehn R, Funamoto K, Fukumoto M, Oki K, Mori S, Hayase T, Kodama T.
  • Organizer: SMIT 2007
  • Place of Presentation: Sendai
  • Year and Date: 20071120-20071122
• [Presentation] Three-dimensional image of intratumoral neovascular constructed with high-frequency contrast-enhanced micro-ultrasound (2007)
  • Author(s): Tomita N, Horie S, Watanabe Y, Funamoto K, Hayase T, Oki K, Morikawa H, Ono M, Mori S, Kodama T.
  • Organizer: SMIT 2007
  • Place of Presentation: Sendai
  • Year and Date: 20071120-20071122
• [Book] Chemical Pollution in Indochina ; Contamination Status, Ecosystem Impact and Remediation Technology(Ed. By Suzuki S and Takada H.) (2009)
  • Author(s): Komori H, Soga Y, Mori S, Nose M
  • Total Pages: 160-167
  • Publisher: Tokai University Press
• [Patent(Industrial Property Rights)] 超音波発生用プローブ (2007)
  • Inventor(s): 小玉哲也, 森士朗
  • Industrial Property Rights Holder: 国立大学法人東北大学、日本素材株式会社、株式会社マテリアル仙台
  • Industrial Property Number: PCT国際出願PCT/JP2007/057878
  • Filing Date: 2007-04-10
  • Overseas