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2008 Fiscal Year Final Research Report

Tissue-engineered tooth based on epithelial cell rest of Malassez and dental pulp cells using tissue engineering technology

Research Project

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Project/Area Number 19390511
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionNihon University (2008)
The University of Tokyo (2007)

Principal Investigator

HONDA Masaki  Nihon University, 歯学部, 専任講師 (70361623)

Co-Investigator(Renkei-kenkyūsha) KAGAMI Hideaki  東京大学, 医科学研究所, 准教授 (80242866)
SAGARA Hiroshi  東京大学, 医科学研究所, 助教 (50145041)
Project Period (FY) 2007 – 2008
Keywordsアメロゲニン / エナメル質再生 / エナメル芽細胞 / 歯髄細胞 / 上皮ー間葉相互作用 / 組織工学的手法 / 歯の再生 / マラッセ上皮遺残細胞
Research Abstract

今回提案した研究課題を通じて、マラッセ上皮遺残細胞の長期間の培養に成功した。次に培養マラッセ上皮細胞は歯髄細胞と共培養することで、エナメル芽細胞に分化した。さらに、組織工学的手法に用いて、培養マラッセ上皮細胞と歯髄細胞を移植すると、移植体の中にエナメル質が形成した。これらの結果から、マラッセ上皮遺残細胞がエナメル質形成能力を持っていることが確認でき、予定した研究課題を成就できたと考えられる。

  • Research Products

    (22 results)

All 2009 2008 2007

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (10 results) Book (3 results)

  • [Journal Article] Enamel Tissue Engineering Using Subcultured Enamel Organ Epithelial Cells in Combination with Dental Pulp Cells2009

    • Author(s)
      Honda MJ, Shinmura Y, Shinohara Y
    • Journal Title

      Cells Tissues Organs 189(1-4)

      Pages: 261-267

    • Peer Reviewed
  • [Journal Article] The location and characteristics of two populations of dental pulp cells affect tooth development2009

    • Author(s)
      Sumita Y, Asahina I, Kagami H, Honda MJ
    • Journal Title

      European Journal of Oral Sciences 117(2)

      Pages: 113-121

    • Peer Reviewed
  • [Journal Article] The induction of dentin bridge-like structures by constructs of subcultured dental pulp-derived cells and porous HA/TCP in porcine teeth2009

    • Author(s)
      Ando Y, Honda MJ, Ohshima H, Tonomura A, Ohara T, Itaya T, Kagami H
    • Journal Title

      Nagoya J 71

      Pages: 51-62

    • Peer Reviewed
  • [Journal Article] Quiescent epithelial cell rest of Malassez can differentiate into ameloblast-like cells2008

    • Author(s)
      Shinmura Y, Tsuchiya S, Hata KI, Honda MJ
    • Journal Title

      J Cell Phy 217(3)

      Pages: 728-738

    • Peer Reviewed
  • [Journal Article] Collagen type I matrix affects the molecular and cellular behavior of purified porcine dental follicle cells2008

    • Author(s)
      Tuchiya S, Honda MJ, Shinohara Y, Saito M, Ueda M
    • Journal Title

      Cell & Tissue Research 331

      Pages: 447-459

    • Peer Reviewed
  • [Journal Article] Tooth-forming potential in embryonic and postnatal tooth bud cells2008

    • Author(s)
      Honda MJ, Fong H, Iwatsuki S, Sumita Y, Sarikaya M
    • Journal Title

      Med Mol Morphol 41(4)

      Pages: 183-192

    • Peer Reviewed
  • [Journal Article] Side population cells expressing ABCG2 in human adult dental pulp tissue2007

    • Author(s)
      Honda MJ, Nakashima F, Satomura K, Shinohara Y, Tsuchiya S, Watanabe N, Ueda M
    • Journal Title

      International Endodontic Journal 40(12)

      Pages: 949-58

    • Peer Reviewed
  • [Journal Article] Subcultured odontogenic epithelial cells in combination with dental mesenchymal cells produce enamel-dentin-like complexes structures2007

    • Author(s)
      Honda, MJ, Shinohara Y, Hata KI, Ueda M
    • Journal Title

      Cell Transplantation 16(8)

      Pages: 833-47

    • Peer Reviewed
  • [Journal Article] The sequential seeding of epithelial and mesenchymal cells for tissue-engineered tooth regeneration2007

    • Author(s)
      Honda MJ, Tsuchiya S, Sumita Y, Sagara H, Ueda M
    • Journal Title

      Biomaterials 28

      Pages: 680-689

    • Peer Reviewed
  • [Presentation] Epithelial cell rest of Malassez have a potential to produce enamel2008

    • Author(s)
      Honda M. et.al.
    • Organizer
      86^<th> International Academy of Dental Research
    • Place of Presentation
      General Session, Toronto, Canada
    • Year and Date
      20080702-05
  • [Presentation] Porcine epithelial cell rest of Malassez have the potential to produce enamel tissue2008

    • Author(s)
      Masaki Honda
    • Organizer
      Gordon Research Conference on CRANIOFACIAL MORPHOGENESIS & TISSUE REGENERATION
    • Place of Presentation
      Gordon Research Conference on CRANIOFACIAL MORPHOGENESIS & TISSUE REGENERATION
    • Year and Date
      20080209-14
  • [Presentation] 培養歯胚細胞を用いた歯の再生研究2008

    • Author(s)
      本田雅規
    • Organizer
      平成20年度日本歯科大学歯学大会
    • Year and Date
      2008-06-07
  • [Presentation] Subcultured dental enamel organ epithelial cells in combination with dental papilla cells produce enamel-dentin like complexes structures2007

    • Author(s)
      Honda M
    • Organizer
      9^<th> International conference of the chemistry and biology of mineralized tissues
    • Place of Presentation
      Texas, USA
    • Year and Date
      20071104-08
  • [Presentation] マラッセ上皮遺残細胞はエナメル質を形成する2007

    • Author(s)
      新村優佳
    • Organizer
      第50回日本歯周病学会
    • Place of Presentation
      東京
    • Year and Date
      20070920-22
  • [Presentation] 長期培養したマラッセ上皮遺残細胞の特性の解析2007

    • Author(s)
      新村優佳
    • Organizer
      第49回歯科基礎医学会
    • Place of Presentation
      札幌
    • Year and Date
      20070829-31
  • [Presentation] 培養歯胚上皮細胞を用いたエナメルーデンチン構造の再生2007

    • Author(s)
      本田雅規
    • Organizer
      第61回日本口腔科学会
    • Place of Presentation
      神戸
    • Year and Date
      20070419-20
  • [Presentation] Tissue engineering of enamel-dentin complex structures2007

    • Author(s)
      Masaki Honda, et.al.
    • Organizer
      85^<th> General session & Exhibition of the IADR
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      20070321-24
  • [Presentation] Enamel grow from porcine cultured dental epithelial cells2007

    • Author(s)
      Honda M
    • Organizer
      The 26^<th> Korean Division of the International Association for Dental Research
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2007-11-29
  • [Presentation] Tissue engineered dentin/enamel complex using cultured dental epithelial cells and primary dental papilla/pulp cells2007

    • Author(s)
      Honda M
    • Organizer
      International Symposium on Molecular Destruction and Reconstruction of the Dentin/Pulp Complex and Its Surrounding Tissues
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-03-05
  • [Book] Tooth-Tissue Engineering(chapter)in Tissue Engineering Research Trends, Nova Science Publishers2008

    • Author(s)
      Honda MJ
    • Total Pages
      267-279
    • Publisher
      Inc, New York
  • [Book] 組織工学的手法による歯の再生2008

    • Author(s)
      本田雅規
    • Total Pages
      19-24
    • Publisher
      科学と工業82(5)
  • [Book] 歯の再生. 再生医療技術の最前線(バイオテクノロジーシリーズ)2007

    • Author(s)
      本田雅規
    • Total Pages
      227-233
    • Publisher
      シーエムシー出版(東京)

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Published: 2010-06-10   Modified: 2016-04-21  

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