Molecular and genetic analysis of osteoclast differentiantion factor and osteoclastgenesis inhibitory factor in osteoporosis and control of inhibition of bone resorption by 1, 4-dihydroxy-2-naphthoic acid(DHNA)

2008 Fiscal Year Final Research Report

• Project/Area Number: 19390516
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Aichi Gakuin University (2008); Okayama University (2007)
• Principal Investigator: SUGAHARA Toshio Aichi Gakuin University, 歯学部, 客員教授 (10116048)
• Co-Investigator(Kenkyū-buntansha): MISHIMA Katsuaki 岡山大学病院, 口腔外科, 講師 (60304317) ; UENO Takaaki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (60252996) ; HIRATA Azumi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (40263587) ; MATSUMURA Tatsushi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70432648) ; MINAMI Katsuhiro 愛知学院大学, 歯学部, 講師 (70346162) ; FUJIWARA Kumiko 愛知学院大学, 歯学部, 非常勤助教 (60404737)
• Project Period (FY): 2007 – 2008
• Keywords: 骨粗鬆症 / ビフィズス菌 / プロピオン酸 / DHNA / 骨免疫 / 骨吸収

Research Abstract

免疫抑制剤FK506投与による骨粗懸症に対するプロピオン酸菌の発酵産物(PC)である新規なビフィズス菌増殖因子1, 4-dihydroxy-2-naphthoic acid(DHNA)の効果の検討した。
1. DHNAの骨粗鬆症改善効果を証明した。
2. この効果はFK506投与により増加したB細胞を減少させ、減少したT細胞を増加させることにより起こると考えられた。
3. 骨髄から、マイクロアレイを用いた網羅的解析を行ない、対照群と比較し発現差が認められたのは、骨免疫に関与している2487個の遺伝子であった。

Research Products

• [Journal Article] Application of a novel method to analyze lip motion to cleft lip patients before and after lip repair (2009)
  • Author(s): Mishima K., Yamada T., Sugii A., Matsumura T., Sugahara T.
  • Journal Title: Dentomaxillofacial Radiology (in press)
  • Peer Reviewed
• [Journal Article] Localization of Runx2, osterix and osteopontin in tooth root formation in Rat Molars (2009)
  • Author(s): Hirata, A., Sugahara, T. and Nakamura, H.
  • Journal Title: Journal of Histochemistry and Cytochemistry, (Epub ahead of print) 57 Pages: 397-403
  • Peer Reviewed
• [Journal Article] A histological comparison of bone induced from grafted periosteum with bone induced from grafted bone marrow in the rat calvarial defect model (2008)
  • Author(s): Ueno T, Honda K, Hirata A, Kagawa T, Kanou M, Shirasu N, Sugahara T
  • Journal Title: Acta Histochmeica 110(3) Pages: 217-223
  • Peer Reviewed
• [Journal Article] Hepkakenn ranase contributes to palate fusion by degradind perlecan (2008)
  • Author(s): A. Hirata, H. Imura, T. Yamada, T. Ueno, E. Yamachika, N. Moritani, T. Matsumura, T. Kagawa, K. Mishima, T. Sugahara
  • Journal Title: Jounal of Cranio-Maxillofacial Surgery Vol 36, (1) Pages: 173
  • Peer Reviewed
• [Journal Article] Expression of human β-defensin -1, -2, and -3 in non-inflamed pseudocyst, mucoceles (2008)
  • Author(s): MK Frederic, T Yamai, N Mizukawa, Y Kaneda, N Katase, M Gunduz, H Nagatsuka, T Sugahara
  • Journal Title: Oral Diseases 14 Pages: 652-657
  • Peer Reviewed
• [Journal Article] Morphological and immunohistochemical study on cleft palate induced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin in mice (2008)
  • Author(s): Fujiwara, K., Yamada, T., Mishima, K., Imura, H., Sugahara, T.
  • Journal Title: Congenital Anomalies 48(2) Pages: 68-73
  • Peer Reviewed
• [Journal Article] Relationships between nasalance scores and nasopharyngeal shapes in cleft palate patients (2008)
  • Author(s): Katsuaki Mishima, Tomohiro YAMADA, Asuka SUGII, Hideto IMURA and Toshio SUGAHARA
  • Journal Title: J Cranio-Maxillofacial Surgery 36(1) Pages: 11-14
  • Peer Reviewed
• [Journal Article] Dialectal and gender differences in nasalance scores in a Japanese population (2008)
  • Author(s): Katsuaki Mishima, Asuka SUGII, Tomohiro YAMADA, Hideto IMURA and Toshio SUGAHARA
  • Journal Title: J Cranio-Maxillofacial Surgery 2008b 36(1) Pages: 8-10
  • Peer Reviewed
• [Journal Article] Histological comparison of bone induced from autogenously grafted periosteum with bone induced from autogenously grafted bone marrow in the rat calvarial defect model (2008)
  • Author(s): Ueno, T., Honda, K., Hirata, A., Kagawa, T., Kanou, M., Shirasu, N., Sawaki, M., Yamachika, E., Mizukawa, N. and Sugahara, T.
  • Journal Title: Acta Histochmeica 110 Pages: 217-223
  • Peer Reviewed
• [Journal Article] Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)on the development of the murine palate in organ culture (2007)
  • Author(s): Yamada, T., Fujiwara, K., Mishima, K., Imura, H., Sugahara, T.
  • Journal Title: Asian J Oral Maxillofac Surg 19(4) Pages: 192-196
  • Peer Reviewed
• [Journal Article] The evaluation of bone formation of the whole tissue periosteum transplantation in combination with betatricalcium phosphate(TCP) (2007)
  • Author(s): Ueno. T., Sakata, Y., Hirata, A., Kagawa, T., Kanou, M., Shirasu, N., Sawaki, M., Fukunaga, J., Mizukawa, N. and Sugahara, T.
  • Journal Title: Annals of Plastic Surgery 59 Pages: 707-712
  • Peer Reviewed
• [Journal Article] Evaluation of Osteogenic Potential of Cultured Periosteum Derived Cells-Preliminary Animal Study- (2007)
  • Author(s): Ueno. T., Kagawa, T., Kanou, M., Shirasu, N., Sawaki, M., Imura, H., Hirata, A., Yamachika, E., Mizukawa, N. and Sugahara, T.
  • Journal Title: Journal of Hard Tissue Biology 16 Pages: 50-53
  • Peer Reviewed
• [Presentation] Dental Implant treatment for the patients with Cleft Lip and Palate (2008)
  • Author(s): K. Minami, H. Imura, T. Niimi, H. Furukawa, K. Fujiwara, Y. Mori, T. Sugahara, N. Natsume
  • Organizer: 8th Asian Congress on Oral and Maxillogacial Surgery 2008
  • Place of Presentation: Thailand
  • Year and Date: 20081103-07
• [Presentation] TCDD exposed fused palates had various phenomena (2008)
  • Author(s): H. Imura, T. Yamada, A. Hirata, K. Mishima, K. Minami, H. Furukawa, T., Niimi, K. Fujiwara, N. Natsume, T. Sugahara
  • Organizer: 8th Asian Congress on Oral and Maxillogacial Surgery 2008
  • Place of Presentation: Thailand
  • Year and Date: 20081103-07
• [Presentation] ヘパラナーゼは基底膜パールカンよりbFGFを遊離することで口蓋突起癒合に関与する (2008)
  • Author(s): 平田あずみ、井村英人、山田朋弘、植野高章、山近英樹、森谷徳文、松村達志、香川智正、三島克章、菅原利夫
  • Organizer: 第53回日本口腔外科学会総会
  • Place of Presentation: 徳島市
  • Year and Date: 20081020-21
• [Presentation] Heparanase contributes to palate fusion by degragind perlecan (2008)
  • Author(s): A. Hirata, H. Imura, T. Yamada, T. Ueno, E. Yamachika, N. Moritani, T. Matsumura, T. Kagawa, K. Mishima, T. Sugahara
  • Organizer: 19th Congress of the European Association for Cranio- Maxillofacial Surgery
  • Place of Presentation: Bologna, Italy
  • Year and Date: 20080909-12
• [Presentation] Osteogenic potential of grafted periosteum and bone marrow (2008)
  • Author(s): T. Ueno, A. Hirata, T. Kagawa, M. Wakita, M. Kanou, N. Shirasu, M. Sawaki, M. Moritani, Y. Yamachika, M. Mizukawa, T. Sugahara
  • Organizer: 19th Congress of the European Association for Cranio-Maxillofacial Surgery
  • Place of Presentation: Bologna, Italy
  • Year and Date: 20080909-12