Comprehensive study to explore new physiologically active proteins in the snake venoms in the tropical and subtropical regions

2010 Fiscal Year Final Research Report

• Project/Area Number: 19406002
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Biological pharmacy
• Research Institution: Meiji Pharmaceutical University
• Principal Investigator: MORITA Takashi Meiji Pharmaceutical University, 薬学部, 客員研究員 (90128108)
• Co-Investigator(Kenkyū-buntansha): YAMAZAKI Yasuo 明治薬科大学, 薬学部, 助教 (30308621)
• Project Period (FY): 2007 – 2010
• Keywords: 生理活性 / 蛋白質 / プロテアーゼインヒビター / 神経毒蛋白質 / VEGF

Research Abstract

We have performed to study a diverse range of interesting proteins such as procoagulants, anticoagulants, platelet agonists, and platelet antagonists in the venoms of venomous snakes in the both tropical and sub-tropical region. In addition, we have comprehensively studied on the search, isolation, characterization of new C-type lectin-like proteins, cysteine-rich secretory proteins (CRISPs). We finally isolated and characterized more than ten structurally and functionally novel proteins with anticoagulant, antiplatelet or VEGF-activities. These molecules are known to exhibit potent and unique biological activities.

Research Products

• [Journal Article] Snake venom vascular endothelial growth factors (VEGF-Fs) exclusively vary their structures and functions among species. (2009)
  • Author(s): Yamazaki Y, Matsunaga Y, Tokunaga Y, Obayashi S, Saito M, Morita T
  • Journal Title: J.Biol.Chem. 284 Pages: 9885-9891
  • Peer Reviewed
• [Journal Article] VEGF-A and VEGF-F evoke distinct changes in vascular ultrastructure. (2009)
  • Author(s): Matsunaga Y, Yamazaki Y, Suzuki H, Morita T
  • Journal Title: Biochem.Biophys.Res.Commun. 379 Pages: 872-875
  • Peer Reviewed
• [Journal Article] Structural divergence of cysteine-rich secretary proteins in snake venoms. (2009)
  • Author(s): Matsunaga Y, Yamazaki Y, Hyodo F, Nozaki M, Morita T
  • Journal Title: J.Biochem. 145 Pages: 365-375
  • Peer Reviewed
• [Journal Article] Anticoagulant mechanism of factor IX/X-binding protein isolated frm the venom of Trimerresurus flavoviridis. (2009)
  • Author(s): Ishikawa M, Kumashiro M, Yamazaki Y, Atoda H, Morita T
  • Journal Title: J.Biochem. 145 Pages: 123-128
  • Peer Reviewed
• [Journal Article] Catalytically inactive phospholipase A2 homologue binds to vcascular endothelial growth factor receptor-2 via a C-terminal loop region. (2008)
  • Author(s): Fujisawa D, Yamazaki Y, Lomonte B, Morita T
  • Journal Title: Biochem.J. 411 Pages: 515-522
  • Peer Reviewed
• [Journal Article] Adenovirus serotype 5 hexon mediates liver gene transter. (2008)
  • Author(s): Waddington SN, McVey JH, Bhella D, Parker AL, Barker K, Atoda H, Pink R, Buckley SMK, Greig JA, Denby L, Custers J, Morita T, Francischetti IMB, Monteiro RQ, Barouch DH, Rooijen NV, Napoli C, Havenga MJE, Nicklin SA, Baker AH
  • Journal Title: Cell 132 Pages: 397-409
  • Peer Reviewed
• [Journal Article] Structures of pseudechetoxin and pseudecin, two snake-venom cysteine-rich secretory proteins that target cyclic nucleotide-gated ion channels : implications for movement of the C-terminal cysteine-rich domain. (2008)
  • Author(s): Suzuki N, Yamazaki Y, Brown RL, Fujimoto Z, Morita T, Mizuno H
  • Journal Title: Acta Cryst. D64 Pages: 1034-1042
  • Peer Reviewed
• [Journal Article] Augmentation of vascular permeability of VEGF is enhanced by KDR-binding proteins. (2007)
  • Author(s): Yamazaki Y, Nakano Y, Imamura T, Morita T
  • Journal Title: Biochem.Biophys.Res.Commun. 355 Pages: 693-699
  • Peer Reviewed
• [Journal Article] Involvement of the snake toxin receptor CLEC-2, in podoplanin-mediated platelet activation, by cancer cells (2007)
  • Author(s): Suzuki-Inoue K, Kato Y, Inoue O, Kaneko MK, Mishima K, Yatomi Y, Yamazaki Y, Narimatsu H, Ozaki Y
  • Journal Title: J.Biol.Chem. 282 Pages: 25993-26001
  • Peer Reviewed
• [Journal Article] Age-and gender-related differences of plasma prothrombin activity levels. (2007)
  • Author(s): Sakata T, Okamoto A, Morita T, Kokubo Y, Sato K, Okayama A, Tomoike H, Miyata T
  • Journal Title: Thromb.Haemost. 97 Pages: 1052-1053
  • Peer Reviewed
• [Presentation] 血液凝固IX因子と抗凝固タンパク質(IX/X-bpおよびIX-bp)の相互作用に対するMg2+の影響 (2008)
  • Author(s): 石川みどり、山崎泰男、森田隆司
  • Organizer: 第55回毒素シンポジウム
  • Place of Presentation: 山梨
  • Year and Date: 20080700
• [Presentation] 組織型および毒型VEGF(血管内皮増殖因子)のゲノム構造解析 (2008)
  • Author(s): 山崎泰男、齋藤麻衣、徳永優子、森田隆司
  • Organizer: 第55回毒素シンポジウム
  • Place of Presentation: 山梨
  • Year and Date: 20080700
• [Presentation] 血液凝固IX因子と抗凝固タンパク質(IX/X-bpおよびIX-bp)の相互作用の速度論的解析 (2008)
  • Author(s): 石川みどり、山崎泰男、森田隆司
  • Organizer: 日本薬学会128年会
  • Place of Presentation: 横浜
  • Year and Date: 20080300
• [Presentation] 異なるC末端ドメインを持つVEGFキメラの創製とその生物活性 (2008)
  • Author(s): 夘月博和、山崎泰男、森田隆司
  • Organizer: 日本薬学会128年会
  • Place of Presentation: 横浜
  • Year and Date: 20080300
• [Presentation] 組織型と毒型の血管内皮増殖因子のゲノム構造解析とその進化論的考察 (2008)
  • Author(s): 齋藤麻衣、徳永優子、長谷川芳裕、山崎泰男、森田隆司
  • Organizer: 日本薬学会128年会
  • Place of Presentation: 横浜
  • Year and Date: 20080300
• [Book] Toxins and Hemostasis. (2010)
  • Author(s): Kini RM, Clemetson KJ, Markland FS, Mclane MA, Morita T (Editors.)
  • Total Pages: 1-797
  • Publisher: Springer
• [Book] Recent Advances in Thrombosis and Hemostasis 2008.(Tanaka K, Davie EW eds.)(Snake Venoms and Other Toxic Components Affecting Thrombosis and Hemostasis.) (2008)
  • Author(s): Yamazaki Y, Morita T
  • Total Pages: 462-482
  • Publisher: Springer