Optical imaging of the depolarization wave in the embryonic CNS -Mechanisms regulating the switching from primordial activity to the respiratory rhythm-

2008 Fiscal Year Final Research Report

• Project/Area Number: 19500268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Kanto Gakuin University
• Principal Investigator: MOMOSE-SATO Yoko Kanto Gakuin University, 人間環境学部, 教授 (70251501)
• Co-Investigator(Renkei-kenkyūsha): SATO Katsushige 東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (80291342)
• Project Period (FY): 2007 – 2008
• Keywords: depolarization wave / 自発活動 / 中枢神経系 / 発生 / 光学計測 / 膜電位感受性色素

Research Abstract

発生初期の中枢神経系で見られる広範囲伝播脱分極波から、成体呼吸中枢リズム活動へのスイッチングがどのようなメカニズムで起こるのかを明らかにするために、光学イメージングによる解析を行った。発生の早い時期にはobexから腰髄までの多数の領域から起こっていた自発性脱分極波は、発生の後期にはほとんどが腰仙髄から起こり、その後の広範囲伝播性の消失が契機となって呼吸中枢の自発的リズム活動が発現してくるものと考えられる。

Research Products

• [Journal Article] Origin of the earliest correlated neuronal activity in the chick embryo revealed by optical imaging with voltage-sensitive dyes (2009)
  • Author(s): Momose-Sato,Y., Mochida, H. and Kinoshita, M
  • Journal Title: European Journal of Neuroscience 29 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo (2009)
  • Author(s): Mochida, H., Sato, K. and Momose-Sato, Y.
  • Journal Title: European Journal of Neuroscience 29 Pages: 14-30
  • Peer Reviewed
• [Journal Article] Optical analysis of neural circuit formation in the embryonic brain (2008)
  • Author(s): Sato. K. and Momose-Sato, Y.
  • Journal Title: Clinical and Experimental Pharmacology and Physiology 35 Pages: 706-713
  • Peer Reviewed
• [Journal Article] Using voltage-sensitive dye recording to image the functional development of neuronal circuits in vertebrate embryos (2008)
  • Author(s): Glover, J. C., Sato, K. and Momose-Sato, Y.
  • Journal Title: Developmental Neurobiology 68 Pages: 804-816
  • Peer Reviewed
• [Journal Article] Spontaneous depolarization waves of multiple origins in the embryonic rat CNS (2007)
  • Author(s): Momose-Sato, Y., Sato, K. and Kinoshita, M.
  • Journal Title: European Journal of Neuroscience 25 Pages: 929-944
  • Peer Reviewed
• [Journal Article] Optical mapping of spatiotemporal emergence of functional synaptic connections in the embryonic chick olfactory pathway (2007)
  • Author(s): Sato, K., Kinoshita, M. and Momose-Sato, Y.
  • Journal Title: Neuroscience 144 Pages: 1334-1346
  • Peer Reviewed
• [Journal Article] Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem visualized with voltage-sensitive dye recording (2007)
  • Author(s): Momose-Sato, Y., Kinoshita, M. and Sato, K.
  • Journal Title: Neuroscience 148 Pages: 140-150
  • Peer Reviewed
• [Journal Article] GABA-induced intracellular Ca^<2+> elevation in the embryonic chick brainstem slice (2007)
  • Author(s): Momose-Sato, Y., Kinoshita, M. and Sato, K.
  • Journal Title: Neuroscience Letters 411 Pages: 42-46
  • Peer Reviewed
• [Journal Article] Embryogenetic expression of glossopharyngeal and vagal excitability in the chick brainstem as revealed by voltage-sensitive dye recording (2007)
  • Author(s): Momose-Sato, Y., Kinoshita, M. and Sato, K.
  • Journal Title: Neuroscience Letters 423 Pages: 138-142
  • Peer Reviewed
• [Presentation] Functional organization of the glossopharyngeal and vagus nerve-related nuclei in the embryonic rat brainstem : Optical mapping with voltage-sensitive dyes (2008)
  • Author(s): Sato, K. and Momose-Sato, Y.
  • Organizer: Society for Neuroscience 38th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20081115-19
• [Presentation] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo (2008)
  • Author(s): Momose-Sato, Y., Mochida, H. and Sato, K.
  • Organizer: Society for Neuroscience 38th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20081115-19
• [Presentation] Neuronal excitability to generate spontaneous activity in the medulla and spinal cord of the chick embryo (2008)
  • Author(s): Kinoshita, M., Momose-Sato, Y. and Sato, K.
  • Organizer: Society for Neuroscience 38th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20081115-19
• [Presentation] Regional gradient of neuronal excitability to generate spontaneous activity in the hindbrain of the chick embryo (2008)
  • Author(s): Kinoshita, M., Momose-Sato, Y. and Sato, K.
  • Organizer: 6th FENS Forum of European Neuroscience
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 20080712-16
• [Presentation] 鶏胚脳幹における迷走神経求心線維の機能発達過程の光学的解析 (2008)
  • Author(s): 佐藤勝重、木下雅恵, 佐藤容子
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 東京
  • Year and Date: 20080709-11
• [Presentation] 光学的イメージングによる鶏胚中枢神経系における脱分極波のoriginの同定 (2008)
  • Author(s): 佐藤容子、持田啓、木下雅恵、佐藤勝重
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 東京
  • Year and Date: 20080709-11
• [Presentation] 鶏胚後脳における神経興奮性の部位差 (2008)
  • Author(s): 木下雅恵, 佐藤容子、佐藤勝重
  • Organizer: 第3 回日本神経科学学会
  • Place of Presentation: 東京
  • Year and Date: 20080709-11
• [Presentation] 鶏胚脳幹迷走神経回路網の機能発達過程の光学的解析 (2008)
  • Author(s): 佐藤勝重、木下雅恵, 佐藤容子
  • Organizer: 第85回日本生理学会
  • Place of Presentation: 東京
  • Year and Date: 20080325-27
• [Presentation] 鶏胚中枢神経系における脱分極波のoriginの同定 : 光学的イメージングによる解析 (2008)
  • Author(s): 佐藤容子、持田啓、木下雅恵、佐藤勝重
  • Organizer: 第85回日本生理学会
  • Place of Presentation: 東京
  • Year and Date: 20080325-27
• [Presentation] Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem visualized with voltage-sensitive dye recording (2007)
  • Author(s): Sato, K., Kinoshita, M. and Momose-Sato, Y.
  • Organizer: Society for Neuroscience 37th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 20071103-07
• [Presentation] The origin of the depolarization wave at an early phase of the chick development revealed by optical imaging with a voltage-sensitive dye (2007)
  • Author(s): Momose-Sato, Y., Mochida, H., Kinoshita, M. and Sato, K.
  • Organizer: Society for Neuroscience 37th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 20071103-07
• [Presentation] Regulation of spontaneous rhythmic activities in the caudal medulla by the spinal cord in the chick embryo (2007)
  • Author(s): Kinoshita, M., Momose-Sato, Y. and Sato, K.
  • Organizer: Society for Neuroscience 37th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 20071103-07
• [Presentation] 鶏胚脳幹聴覚神経回路網の機能発達過程の光学的解析 (2007)
  • Author(s): 佐藤勝重、Glover J. C., 佐藤容子
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 横浜
  • Year and Date: 20070910-12
• [Presentation] ラット胚中枢神経系における自発興奮波の時空間パターンとoriginの同定 : 光学的イメージングによる解析 (2007)
  • Author(s): 佐藤容子、佐藤勝重、木下雅恵
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 横浜
  • Year and Date: 20070910-12
• [Presentation] ラット皮質線条体における膜電位感受性色素による神経伝達イメージング (2007)
  • Author(s): 稲次基希、佐藤勝重、成相直、百瀬-佐藤容子、大野喜久郎
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 横浜
  • Year and Date: 20070910-12
• [Presentation] Depolarization waves in the embryonic CNS visualized with voltage-sensitive dye imaging (2007)
  • Author(s): Momose-Sato, Y., Kinoshita, M. and Sato, K.
  • Organizer: 7th IBRO World Congress of Neuroscience
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20070712-17
• [Presentation] Intraoperative intrinsic optical imaging in brain tumor surgery (2007)
  • Author(s): Inaji, M., Nariai, T., Sato, K., Momose-Sato, Y., Maehara, T. and Ohno, K.
  • Organizer: 6th Congress of the Asian Society for Stereotactic, Functional and Computer Assisted Neurosurgery
  • Place of Presentation: Fujiyoshida, Japan
  • Year and Date: 20070522-24