Three-dimensional structural analysis of Creutzfeldt-Jakob disease-related molecules

2008 Fiscal Year Final Research Report

• Project/Area Number: 19500339
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: MORONE Nobuhiro National Center of Neurology and Psychiatry, 神経研究所・微細構造研究部, 室長 (50399680)
• Project Period (FY): 2007 – 2008
• Keywords: 脳神経疾患 / タンパク質 / 細胞・組織

Research Abstract

クロイツフェルト・ヤコブ病の感染に関わる分子としては、プルシナー博士による「タンパク質仮説」が有力視されているが、詳しい素過程は解明されていない。本研究では、この診断マーカーである「14-3-3ζタンパク質」がミトコンドリアに濃縮していることや、シャペロンタンパク質との複合体形成が「膜中コレステロール濃度の低下」や「アクチンフィラメントの脱重合」により解離することを明らかにした。

Research Products

• [Journal Article] Three-dimensional molecular architecture of the plasma-membrane-associated cytoskeleton as reconstructed by freeze-etch electron tomography. (2008)
  • Author(s): Morone N, Nakada C, Umemura Y, Usukura J, Kusumi A.
  • Journal Title: Methods Cell Biol. 88 Pages: 207-36
• [Journal Article] athematical morphology に基づくバイオイメージからの構造情報の抽出解析 (2008)
  • Author(s): 木森義隆, 諸根信弘, 片山栄作
  • Journal Title: 顕微鏡 44 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research. (2008)
  • Author(s): Kobayashi T, Morone N, Kashiyama T, Oyamada H, Kurebayashi N, Murayama T.
  • Journal Title: PLoS ONE 3 Pages: e3822
  • Peer Reviewed
• [Presentation] GPI-Anchored Protein And Membrane Structure Inside Human Neural Progenitors. (2008)
  • Author(s): Morone N, Wakui F, Kohno T, Yamamura T, Satoh J, and Yuasa S.
  • Organizer: The 5^<th> international Forum on Oxidative Stress and Aging
  • Place of Presentation: Ancona Italy.
  • Year and Date: 20080000
• [Presentation] GPI-anchored Protein's complex and membrane skeleton in human neural progenitors as revealed by electron microscopy. (2008)
  • Author(s): Morone N, Wakui F, Kohno T, Kimori Y, Yuasa S.
  • Organizer: The 48th annual meeting of the American Society for Cell Biology
  • Place of Presentation: Francisco USA.
  • Year and Date: 20080000