Optical analysis of functional development of the auditory andvestibular neural circuits

2008 Fiscal Year Final Research Report

• Project/Area Number: 19500346
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: SATO Katsushige Tokyo Medical and Dental University, 大学院医歯学総合研究科, 講師 (80291342)
• Co-Investigator(Renkei-kenkyūsha): SATO Yoko 関東学院大学, 人間環境学部, 教授 (70251501)
• Project Period (FY): 2007 – 2008
• Keywords: 光学計測 / 膜電位感受性色素 / 中枢神経系 / 機能発生 / embryo / 聴覚 / 平衡覚 / 生体染色

Research Abstract

本研究は、発生初期胚脳幹の聴覚・平衡反射神経回路網に焦点をあて、神経電位活動の光学的イメージング法を用いてその機能構築過程を解析したものである。孵卵5.5-8日の鶏胚から第VIII脳神経を付けたままの標本を作製し、膜電位感受性色素で染色して、聴神経刺激に対する光学応答を記録した。解剖学的研究との比較から、脳幹内での応答領域と神経核の対応を検討した。また、各神経核におけるシナプス伝達の発現時期は、これまで考えられていた発生段階よりもかなり早いことが明らかとなった。

Research Products

• [Journal Article] Origin of the earliest correlatedneuronal activity in the chick embryo revealedby optical imaging with voltage-sensitive dyes (2009)
  • Author(s): Momose-Sato, Y., Mochida, H., Kinoshita, M
  • Journal Title: European Journal of Neuroscience 29 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Switching of the transmitters that mediatehindbrain correlated activity in the chick embryo (2009)
  • Author(s): Mochida, H., Sato, K., Momose-Sato, Y
  • Journal Title: European Journal of Neuroscience 29 Pages: 14-30
  • Peer Reviewed
• [Journal Article] Opticalanalysis of neural circuit formation in theembryonic brain (2008)
  • Author(s): Sato. K., Momose-Sato, Y
  • Journal Title: Clinical and ExperimentalPharmacology and Physiologya 35 Pages: 706-713
  • Peer Reviewed
• [Journal Article] Using voltage-sensitive dye recording toimage the functional development of neuronalcircuits in vertebrate embryos (2008)
  • Author(s): Glover, J. C., Sato, K., Momose-Sato, Y
  • Journal Title: Developmental Neurobiology 68 Pages: 804-816
  • Peer Reviewed
• [Journal Article] Processing of sting pheromone and itscomponents in the antennal lobe of the workerhoneybee (2008)
  • Author(s): Wang, S., Sato, K., Giurfa, M., Zhang, S
  • Journal Title: Journal of Insect physiology 54 Pages: 833-841
  • Peer Reviewed
• [Journal Article] Spontaneous depolarization waves ofmultiple origins in the embryonic rat CNS (2007)
  • Author(s): Momose-Sato, Y., Sato, K., Kinoshita, M
  • Journal Title: European Journal of Neuroscience 25 Pages: 929-944
  • Peer Reviewed
• [Journal Article] Optical mapping of spatiotemporalemergence of functional synaptic connections in the embryonic chick olfactory pathway (2007)
  • Author(s): Sato, K., Kinoshita, M., Momose-Sato, Y
  • Journal Title: Neuroscience 144 Pages: 1334-1346
  • Peer Reviewed
• [Journal Article] Development of vagal afferent projectionscircumflex to the obex in the embryonic chickbrainstem visualized with voltage-sensitive dyerecording (2007)
  • Author(s): Momose-Sato, Y., Kinoshita, M., Sato, K
  • Journal Title: Neuroscience 148 Pages: 140-150
  • Peer Reviewed
• [Journal Article] GABA-induced intracellular Ca^<2+>elevation in the embryonic chick brainstem slice (2007)
  • Author(s): Momose-Sato, Y., Kinoshita, M., Sato, K
  • Journal Title: Neuroscience Letters 411 Pages: 42-46
  • Peer Reviewed
• [Journal Article] Embryogenetic expression of glossopharyngeal and vagal excitability in thechick brainstem as revealed by voltage-sensitivedye recording (2007)
  • Author(s): Momose-Sato, Y., Kinoshita, M., Sato, K
  • Journal Title: Neuroscience Letters 423 Pages: 138-142
  • Peer Reviewed
• [Journal Article] A comparative studyof bioradiography in human brain slices andpreoperative PET imaging (2007)
  • Author(s): Sasaki, T., Nariai, T., Maehara, T., Sato, K., Oda, K., Ishii, K
  • Journal Title: Brain Research 1142 Pages: 19-27
  • Peer Reviewed
• [Presentation] Maehara, T., Inaji, M., Nariai, T., Sato, K. andOhno, K (2008)
  • Author(s): Maehara, T., Inaji, M., Nariai, T., Sato, K. Ohno, K
  • Organizer: AmericanEpilepsy Society 62nd Annual Meeting
  • Place of Presentation: Seattle, USA
  • Year and Date: 20081205-09
• [Presentation] Functionalorganization of the glossopharyngeal and vagusnerve-related nuclei in the embryonic ratbrainstem : Optical mapping withvoltage-sensitive dyes (2008)
  • Author(s): Sato, K., Momose-Sato, Y
  • Organizer: Society for Neuroscience 38th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20081115-19
• [Presentation] Switching of the transmitters that mediatehindbrain correlated activity in the chick embryo (2008)
  • Author(s): Momose-Sato, Y., Mochida, H., Sato, K
  • Organizer: Society for Neuroscience 38th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20081115-19
• [Presentation] Neuronal excitability to generate spontaneousactivity in the medulla and spinal cord of thechick embryo (2008)
  • Author(s): Kinoshita, M., Momose-Sato, Y., Sato, K
  • Organizer: Society for Neuroscience 38thAnnual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20081115-19
• [Presentation] Regional gradient of neuronal excitability togenerate spontaneous activity in the hindbrain of the chick embryo (2008)
  • Author(s): Kinoshita, M., Momose-Sato, Y., Sato, K
  • Organizer: 6th FENS Forum of EuropeanNeuroscience
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 20080712-16
• [Presentation] 鶏胚脳幹における迷走神経求心線維の機能発達過程の光学的解析 (2008)
  • Author(s): 佐藤勝重, 木下雅恵, 佐藤容子
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 東京
  • Year and Date: 20080709-11
• [Presentation] 光学的イメージングによる鶏胚中枢神経系における脱分極波のoriginの同定 (2008)
  • Author(s): 佐藤容子, 持田啓, 木下雅恵, 佐藤勝重
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 東京
  • Year and Date: 20080709-11
• [Presentation] 鶏胚後脳における神経興奮性の部位差 (2008)
  • Author(s): 木下雅恵, 佐藤容子, 佐藤勝重
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 東京
  • Year and Date: 20080709-11
• [Presentation] 鶏胚脳幹迷走神経回路網の機能発達過程の光学的解析 (2008)
  • Author(s): 佐藤勝重, 木下雅恵, 佐藤容子
  • Organizer: 第85回日本生理学会
  • Place of Presentation: 東京
  • Year and Date: 20080325-27
• [Presentation] 鶏胚中枢神経系における脱分極波のoriginの同定 : 光学的イメージングによる解析 (2008)
  • Author(s): 佐藤容子, 持田啓, 木下雅恵, 佐藤勝重
  • Organizer: 第85回日本生理学会
  • Place of Presentation: 東京
  • Year and Date: 20080325-27
• [Presentation] 初期胚脳幹における機能的神経回路網形成に対する光学的アプローチ (2008)
  • Author(s): 佐藤勝重
  • Organizer: 第85回日本生理学会
  • Place of Presentation: 東京
  • Year and Date: 20080325-27
• [Presentation] Development of vagal afferent projectionscircumflex to the obex in the embryonic chickbrainstem visualized with voltage-sensitive dyerecording (2007)
  • Author(s): Sato, K., Kinoshita, M., Momose-Sato, Y
  • Organizer: Society for Neuroscience 37th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 20071103-07
• [Presentation] The origin of the depolarizationwave at an early phase of the chick developmentrevealed by optical imaging with avoltage-sensitive dye (2007)
  • Author(s): Momose-Sato, Y., Mochida, H., Kinoshita, M., Sato, K
  • Organizer: Society for Neuroscience37th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 20071103-07
• [Presentation] Regulation of spontaneous rhythmic activities inthe caudal medulla by the spinal cord in the chickembryo (2007)
  • Author(s): Kinoshita, M., Momose-Sato, Y., Sato, K
  • Organizer: Society for Neuroscience 37th AnnualMeeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 20071103-07
• [Presentation] 鶏胚脳幹聴覚神経回路網の機能発達過程の光学的解析 (2007)
  • Author(s): 佐藤勝重、Glover J.C., 佐藤容子
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 横浜
  • Year and Date: 20070910-12
• [Presentation] ラット胚中枢神経系における自発興奮波の時空間パターンとoriginの同定 : 光学的イメージングによる解析 (2007)
  • Author(s): 佐藤容子, 佐藤勝重, 木下雅恵
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 横浜
  • Year and Date: 20070910-12
• [Presentation] ラット皮質線条体における膜電位感受性色素による神経伝達イメージング (2007)
  • Author(s): 稲次基希, 佐藤勝重, 成相直, 百瀬-佐藤容子, 大野喜久郎
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 横浜
  • Year and Date: 20070910-12
• [Presentation] Chick終脳における刷り込み成立に伴う神経回路活性の解析 (2007)
  • Author(s): 中森智啓, 佐藤勝重, 田中光一, 浜崎浩子
  • Organizer: 第30回日本神経科学学会
  • Place of Presentation: 横浜
  • Year and Date: 20070910-12
• [Presentation] Depolarization waves in the embryonic CNSvisualized with voltage-sensitive dye imaging (2007)
  • Author(s): Momose-Sato, Y., Kinoshita, M., Sato, K
  • Organizer: 7th IBRO World Congress of Neuroscience
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20070612-17
• [Presentation] Optical signalof human cerebral cortex induced by soundstimulation as monitoring tool of parasylvianstructure of dominant hemisphere (2007)
  • Author(s): Nariai, T., Sato, K., Maehara, T., Inaji, M., Hosoda, C., Ishii, K., Ohno, K
  • Organizer: The 13thAnnual Meeting of the Organization for HumanBrain Mapping
  • Place of Presentation: Chicago, USA
  • Year and Date: 20070610-14
• [Presentation] Intraoperativeintrinsic optical imaging in brain tumorsurgery (2007)
  • Author(s): Inaji, M., Nariai, T., Sato, K., Momose-Sato, Y., Maehara, T., Ohno, K
  • Organizer: 6th Congress of the Asian Society forStereotactic, Functional and Computer Assisted Neurosurgery
  • Place of Presentation: Fujiyoshida, Japan
  • Year and Date: 20070522-24
• [Presentation] 光学的イメージング法による鶏胚聴覚神経回路網の機能発達過程の解析 (2007)
  • Author(s): 佐藤勝重, Glover J. C., 佐藤容子
  • Organizer: 第84回日本生理学会
  • Place of Presentation: 大阪
  • Year and Date: 20070320-22
• [Presentation] ラット胚中枢神経系における自発性脱分極波のorigin の光学的解析 (2007)
  • Author(s): 佐藤容子, 佐藤勝重, 木下雅恵
  • Organizer: 第84回日本生理学会
  • Place of Presentation: 大阪
  • Year and Date: 20070320-22
• [Book] H. Approaches to the visualization of neuralpathways in chick imprinting. In : NeuralPathways : New Research. Ed(in press)
  • Author(s): Maekawa, F., Sato, K., Ohki-Hamazaki, H
  • Publisher: Columbus, F. Nova Science Publishers