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2008 Fiscal Year Final Research Report

Molecular meohanisms of mechanosensing inskeletal muscles : Based on regulation system of atrogen expression

Research Project

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Project/Area Number 19500564
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Sports science
Research InstitutionThe University of Tokushima

Principal Investigator

NIKAWA Takeshi  The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 教授 (20263824)

Co-Investigator(Kenkyū-buntansha) YASUI Matsuo  徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (00157984)
NAKAYA Yutaka  徳島大学, 大学院・ヘルスバシオサイエンス研究部, 教授 (50136222)
Project Period (FY) 2007 – 2008
Keywordsユビキチンリガーゼ / 機械的ストレスの感知 / IGF-1シグナル / 運動器(筋・骨) / 蛋白質分解酵素
Research Abstract

Cb1-bの発現が運動器の活動状態(機械的ストレス)と相関する非常に良い指標になることがわかった。この新しい知見は、Cb1-bの発現調節機序を解明できれば機械的ストレスに対する運動器の感知機構が明らかになることを強く示唆した。そこで、本研究で、Cb1-b遺伝子のプロモーター解析を行った結果、Egr-1とSp1の転写因子がCb1-bの発現をそれぞれ正と負に制御していることがわかった。さらに、これら転写因子の上流を解析すると、MAPK経路とくにERK-1/2経路がEgrの活性を誘導していた。ERK-1/2を活性化するものこそが、運動器の活動状態(機械的ストレス)の感知機構の重要なセンサーであることがほぼ明らかにした。申請者らは、それが膜上の蛋白質分解酵素ではないかという新たなメカニズムを提唱している。

  • Research Products

    (25 results)

All 2009 2008 2007 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (4 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Awa(Tokushima)lactate-fermented tea as well as green tea enhance the effect of diet restriction on obesity in rats2009

    • Author(s)
      Sogawa M, Seura T, Kohno S, Hirasaka K, Yamaguchi Y, Takagi R, Harada A, Okumura Y, Yamamoto S, Kishi K, Nikawa T
    • Journal Title

      J Med Invest 56(1-2)

      Pages: 42-48

    • Peer Reviewed
  • [Journal Article] Polyphenols prevent clinorotation-induced expression of atrogenes in mouse C2C12 skeletal myotubes2009

    • Author(s)
      Hemdan DII, Hirasaka K, Nakao R, Kohno S, Kagawa S, Abe T, Harada-Sukeno A, Okumura Y, Nakaya Y, Terao J, Nikawa T
    • Journal Title

      J Med Invest 56(1-2)

      Pages: 26-32

    • Peer Reviewed
  • [Journal Article] Glucose infusion suppresses surgery-induced muscle protein breakdown by inhibiting ubiquitin-proteasome pathway in rats2009

    • Author(s)
      Mikura M, Yamaoka I, Doi M, Kawano Y, Nakayama M, Nakao R, Hirasaka K, Okumura Y, Nikawa T
    • Journal Title

      Anesthesiology 110(1)

      Pages: 81-8

    • Peer Reviewed
  • [Journal Article] Effects of Dimethyl Sulphoxide and Dexamethasone on mRNA Expression of Myogenesis- and Muscle Proteolytic System-related Genes in Mouse Myoblastic C2C12 Cells2008

    • Author(s)
      Nishimura M, Mikura M, Hirasaka K, Okumura Y, Nikawa T, Kawano Y, Nakayama M, Ikeda M
    • Journal Title

      J Biochem 144(6)

      Pages: 717-724

    • Peer Reviewed
  • [Journal Article] athepsin C propeptide interacts with intestinal alkaline phosphatase and heat shock cognate protein 70 in human Caco-2 cells2008

    • Author(s)
      Hirasaka K, Tokuoka K, Nakao R, Yamada C, Oarada M, Imagawa T, Ishidoh K, Okumura Y, Kishi K, Nikawa T
    • Journal Title

      Physiol Sci 58(2)

      Pages: 105-11

    • Peer Reviewed
  • [Journal Article] Subcutaneous injection, from birth, of epigallocatechin-3-gallate, a component of green tea, limits the onset of muscular dystrophy in mdx mice : a quantitative histological, immunohistochemical and electrophysiological study2008

    • Author(s)
      Nakae Y, Hirasaka K, Goto J, Nikawa T, Shono M, Yoshida M, Stoward PJ
    • Journal Title

      Histochem Cell Biol 129(4)

      Pages: 489-501

    • Peer Reviewed
  • [Journal Article] Effects of dietary fish oil on lipid peroxidation and serum triacylglycerol levels in psychologically stressed mice2008

    • Author(s)
      Oarada M, Tsuzuki T, Gonoi T, Igarashi H, Kamei K, Nikawa T, Hirasaka K, Ogawa T, Miyazawa T, Nakagawa K, Kurita N
    • Journal Title

      Nutrition 24(1)

      Pages: 67-75

    • Peer Reviewed
  • [Journal Article] Effects of Dimethy1 Sulfoxide and Dexamethasone on mRNA Expression of Housekeeping Genes in Cultures of C2C12 Myotubes2008

    • Author(s)
      Masuhiro Nishimura M, Nikawa T, Kawano Y, Nakayama M, Muneharu Ikeda M
    • Journal Title

      Biochem Biophys Res Commun 367(3)

      Pages: 603-8

    • Peer Reviewed
  • [Journal Article] Effects of dietary fish oil on lipid peroxidation and serum triacylglycerol levels in psychologically stressed mice2008

    • Author(s)
      Oarada M, Tsuzuki T, Gonoi T, Igarashi H, Kamei K, Nikawa T, Hirasaka K, Ogawa T, Miyazawa T, Nakagawa K, Kurita N
    • Journal Title

      Nutrition 24 (1)

      Pages: 67-75

    • Peer Reviewed
  • [Journal Article] Peroxidized cholesterol-induced matrix metalloproteinase-9 activation and its suppression by dietary beta-carotene in photoaging of hairless mouse skin2008

    • Author(s)
      Minami Y, Kawabata K, Kubo Y, Arase S, Hirasaka K, Nikawa T, Banda N, Kawai Y, Terao J
    • Journal Title

      J Nutr Biochem 23

    • Peer Reviewed
  • [Journal Article] Osteoactivin fragments produced by ectodomain shedding induce MMP-3 expression via ERK pathway in mouse NIH-3T3 fibroblasts2007

    • Author(s)
      Furochi H, Tamura S, Mameoka M, Yamada C, Ogawa T, Hirasaka K, Okumura Y, Imagawa T, Oguri S, Ishidoh K, Kishi K, Higashiyama S, Nikawa T
    • Journal Title

      FEBS Lett 581(30)

      Pages: 5743-50

    • Peer Reviewed
  • [Journal Article] Deficiency of Cb1-b gene enhances infiltration and activation of macrophages in adipose tissue and causes peripheral insulin resistance in mice2007

    • Author(s)
      Hirasaka K, Kohno S, Goto J, Furochi H, Mawatari K, Hamada N, Hosaka T, Nakaya Y, Ishidoh K, Obata T, Ebina Y, Gu H, Takeda S, Kishi K, Nikawa T
    • Journal Title

      Diabetes 56(10)

      Pages: 2511-22

    • Peer Reviewed
  • [Journal Article] istinct effects of anterior pyriform cortex and the lateral hypothalamus lesions on protein intake in rats2007

    • Author(s)
      Nakao R, Ozaki E, Hasegawa M, Kondo A, Uezu K, Hirasaka K, Nikawa T, Kishi K
    • Journal Title

      J Med Invest 54(3-4)

      Pages: 255-60

    • Peer Reviewed
  • [Journal Article] Overexpression of osteoactivin protects skeletal muscle from severe degeneration caused by long-term denervation in mice2007

    • Author(s)
      urochi H, Tamura S, Takeshima K, Hirasaka K, Nakao R, Kishi K, Nikawa T
    • Journal Title

      J Med Invest 54(3-4)

      Pages: 248-54

    • Peer Reviewed
  • [Journal Article]2007

    • Author(s)
      Nakano S, Mishiro T, Takahara S, Yokoi H, Hamada D, Yukata K, Takata Y, Goto T, Egawa H, Yasuoka S, Furouchi H, Hirasaka K, Nikawa T, Yasui N. Nakano S, Mishiro T, Takahara S, Yokoi H, Hamada D, Yukata K, Takata Y, Goto T, Egawa H, Yasuoka S, Furouchi H, Hirasaka K, Nikawa T, Yasui N
    • Journal Title

      Clin Rheumatol 26(8)

      Pages: 1284-92

    • Peer Reviewed
  • [Journal Article] Effects of single administration of Rokumi-gan (TJ-87) on serum amino acid concentration of 6 healthy Japanese male volunteers2007

    • Author(s)
      akahashi H, Nakao R, Hirasaka K, Kishi K, Nikawa T
    • Journal Title

      J Med Invest 54(1-2)

      Pages: 91-8

    • Peer Reviewed
  • [Journal Article] Effect of dietary oils on lymphocyte immunological activity in psychologically stressed mice2007

    • Author(s)
      Oarada M, Gonoi T, Tsuzuki T, Igarashi M, Hirasaka K, Nikawa T, Onishi Y, Toyotome T, Kamei K, Miyazawa T, Nakagawa K, Kashima M, Kurita N
    • Journal Title

      iosci Biotechnol Biochem 71(1)

      Pages: 174-82

    • Peer Reviewed
  • [Presentation] Development of a functional food or drug against unloading-mediated muscle atrophy2008

    • Author(s)
      二川健
    • Organizer
      37th COSPAR Scientific Assembly
    • Place of Presentation
      モントリオール・カナダ
    • Year and Date
      20080713-20
  • [Presentation] Sensing mechanism of unloading stress in skeletal muscle cells2008

    • Author(s)
      二川健
    • Organizer
      第4回日韓宇宙フォーラム
    • Place of Presentation
      東京
    • Year and Date
      2008-10-02
  • [Presentation] Unloadingによる筋萎縮の主要な制御因子2007

    • Author(s)
      二川健
    • Organizer
      Unloadingによる筋萎縮の主要な制御因子
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
  • [Presentation] 宇宙における細胞培養実験の必要性と問題点2007

    • Author(s)
      二川健
    • Organizer
      第13回宇宙医学研究推進分科会 : 宇宙基礎医学生物学研究に用いるべき最適なモデル生物に関するワークショップ
    • Place of Presentation
      東京
    • Year and Date
      2007-12-11
  • [Book] 運動器の生物学と生体力学各組織の力学特性と病態-骨格筋2008

    • Author(s)
      二川健、安井夏生(分担)
    • Total Pages
      500
    • Publisher
      中山書店最新整形外科学大系
  • [Book] 運動器の生物学と生体力学筋・腱-構造と筋収縮の生理学2008

    • Author(s)
      松浦哲也、二川健(分担)
    • Total Pages
      500
    • Publisher
      中山書店最新整形外科学大系
  • [Book] 栄養・食糧学用語辞典2007

    • Author(s)
      二川健(分担)
    • Total Pages
      125-126
    • Publisher
      建吊社
  • [Remarks]

    • URL

      http://150.59.76.31/university/servlet/index/index?&level=4&reference=0/10002/5/30010/20032

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Published: 2010-06-10   Modified: 2016-04-21  

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