2010 Fiscal Year Final Research Report
Cellular immunological activities of antigenic N-glycans from unused seaweed-glycoproteins and it's applications.
| Project/Area Number |
19500701
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Sakuyo Music College |
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Principal Investigator |
KIMURA Mariko Sakuyo Music College, 食文化学部, 准教授 (00351932)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 海藻 / 生理活性 / 抗原性 / 糖鎖 / サイトカイン / 未利用資源 |
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| Research Abstract |
In this study, we analyzed the structures and cellular immunological activities of N-glycans linked to seaweed glycoproteins. First, glycopeptides were prepared from the pepsin-digests of seaweed homogenates (brown algae, red seaweeds, and green laver) by gelfiltration and HPLC. Glycan moieties were liberated by hydrazinolysis and then were fluorescence-labeled. The structural analysis using HPLC-mapping showed that major N-glycans of hydrophytes are typical high-mannose type structures, while the typical plant complex type N-glycans having α1-3Fuc and β1-2 Xyl residues were not found. However, we found some unique glycans that were resistant to several glycosidases, indicating that some hydrophytes-specific glycans might occur on some seaweed glycoproteins. Furthermore, the immunological activity of the seaweed glycopeptides was assayed, and it was found that some glycopeptide-fraction could stimulate human monocytes.
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