2009 Fiscal Year Final Research Report
Detoxification of azo dye waste by Microorganisms
| Project/Area Number |
19510096
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Niihama National College of Technology |
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Principal Investigator |
HAYASE Nobuki Niihama National College of Technology, 生物応用化学科, 教授 (00311100)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Katsuhiko 新居浜工業高等専門学校, 生物応用化学科, 教授 (40149977)
USHIO Kazutoshi 新居浜工業高等専門学校, 生物応用化学科, 教授 (30203508)
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| Project Period (FY) |
2007 – 2009
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| Keywords | アゾ染料 / 微生物分解 / 芳香族アミン |
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| Research Abstract |
Most of aromatic amines resulting from the cleavage of azo dyes are potentially carcinogenic. Therefore aromatic amines are should be treated and detoxified completely. The main objective of this study is to observe microbial decolorization and detoxification of azo dyes. 1) Bradyrhizobium sp. No.168, which degraded sulfanilic acid resulting from the cleavage of azo bond of Orange II, was isolated. The combination of strain No.168 and azo dyes decolorizing microorganisms decolorized Orange II and degraded sulfanilic acid resulting from the decolorization of Orange II. The continuous decolorization of Orange II and degradation of sulfanilic acid was accomplished by immobilized these microorganisms. 2) 4-amino-1-naphtalenesulfonate resulting from the decolorization of Bordeaux S was degraded by laccase produced from white-rot fungi. The continuous degradation of 4-amino-1-naphtalenesulfonat was accomplished by immobilized laccase on alumina beads.
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