Development of a highly sensitive protein detection method using substrates with nano-scale strcture.

2009 Fiscal Year Final Research Report

• Project/Area Number: 19550098
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Tokyo University of Technology
• Principal Investigator: YANO Kazuyoshi Tokyo University of Technology, 応用生物学部, 准教授 (40262109)
• Co-Investigator(Renkei-kenkyūsha): AKIMOTO Takuo 東京工科大学, 応用生物学部, 講師 (90367194)
• Project Period (FY): 2007 – 2009
• Keywords: プロテオミクス / プラズマ重合 / 分析化学 / 薄膜 / 蛍光

Research Abstract

Fluorescence enhancement structure was built up on the glass substrate. First, the Ag film was deposited on the slide glass. The hexamethyldisiloxane plasma-polymerized film (PPF) was subsequently deposited as a dielectric film with various film thickness. The fluorophore-labeled protein was spotted, and the fluorescence intensity from the substrate was measured. As a result, the fluorescence intensity could be enhanced about approximately 13-fold when the thickness of PPF was approximately 56nm, suggesting the future possibility of highly sensitive immunoassay.

Research Products

• [Journal Article] A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring (2009)
  • Author(s): M.-X. Chu, H. Kudo, T. Shirai, K. Miyajima, H. Saito, N. Morimoto, K. Yano, Y. Iwasaki, K. Akiyoshi, K. Mitsubayashi
  • Journal Title: Biomedical Alicroclevices 11(4) Pages: 837-842
  • Peer Reviewed
• [Journal Article] Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis (2007)
  • Author(s): A. Hiratsuka, H. Kinoshita, Y. Maruo, K. Takahashi, S. Akutsu, C. Hayashida, K. Sakairi, K. Usui, K. Shiseki, H. Inamochi, Y. Nakada, K. Yodoya, I. Namatame, Y. Unuma, M. Nakamura, K. Ueyama, Y. Ishii, K. Yano, K. Yokoyama
  • Journal Title: Analytical Chemistry 79 Pages: 5730-5739
  • Peer Reviewed
• [Presentation] プラズマ重合法を用いた抗体アレイの高感度化 (2010)
  • Author(s): 矢野和義、渡辺直、君塚亮太、山野一行、安田充、池下雄介、宮地寛登、秋元卓央
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学
  • Year and Date: 2010-03-27
• [Presentation] プラズマ重合法を用いた抗体アレイの高機能化 (2009)
  • Author(s): 渡辺直、君塚亮太、山野一行、安田充、池下雄介、宮地寛登、秋元卓央、矢野和義
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Patent(Industrial Property Rights)] 不一致増幅物質を含むプローブ核酸を用いる標的配列の検出方法、プローブ核酸および標的配列検出用アッセイキット (2009)
  • Inventor(s): 木下英樹、矢野和義、軽部征夫
  • Industrial Property Rights Holder: 学校法人片柳学園、凸版印刷株式会社
  • Industrial Property Number: 特許・第4283578号
  • Acquisition Date: 2009-03-27