2009 Fiscal Year Final Research Report
Interaction of Lipopolysaccharide and Phospholipid in Mixed Membrane Investigated by Solid-State NMR Spectroscopy
| Project/Area Number |
19550174
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Suntory Institute for Bioorganic Research |
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Principal Investigator |
MIURA Kaoru (野村 薫) Suntory Institute for Bioorganic Research, 研究員 (90353515)
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| Co-Investigator(Kenkyū-buntansha) |
楠本 正一 財)サントリー生物有機科学研究所, 所長 (30028253)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 自然免疫 / リポ多糖 / 固体NMR法 / 脂質二重膜 / 会合状態 / 脂質ラフト |
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| Research Abstract |
We clarified how Lipopolysaccharide (LPS), the ligand of innate immune response, and biomembrane interact by using a solid-state NMR. First, we examined the effect of LPS on membrane morphology and LPS distribution patterns in the membrane based on the miscibility between LPS and individual phospholipid compositions. Then, molecular motions of the LPS and phospholipids were evaluated in the pure phospholipid membrane and raft-forming membrane. Further, we clarified that LPS induces expansion of the raft area in the raft-forming membrane.
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