Immobilized zirconium ion affinity chromatography for specific enrichment of phosphoproteins

2008 Fiscal Year Final Research Report

• Project/Area Number: 19560760
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Properties in chemical engineering process/Transfer operation/Unit operation
• Research Institution: The University of Kitakyushu
• Principal Investigator: UEZU Kazuya The University of Kitakyushu, 国際環境工学部, 教授 (40253497)
• Co-Investigator(Kenkyū-buntansha): 河野 智謙 北九州市立大学, 国際環境工学部, 准教授 (20335699)
• Project Period (FY): 2007 – 2008
• Keywords: リン酸化タンパク質 / W / O / W型界面鋳型重合法金属固定化アフィニティークロマトグラフィー / リン酸化ペプチド

Research Abstract

本研究では、リン酸基と相互作用の強いZr(IV)イオンに着目し、W/O/W型界面鋳型重合法を用いて、樹脂孔表面にZr(IV)を固定させた新規金属固定化アフィニティークロマトグラフィー
(Zr-Phos IMAC)を調製した。マトリックス支援レーザー脱離イオン化法(MALDI-TOF/MS)を用いたモデルタンパク質の分離評価では、従来のIMACよりも非リン酸化ペプチドの検出を軽減させ、リン酸化ペプチドの検出感度を向上させることに成功した

Research Products

• [Journal Article] Shinkai,Molecular Dynamics Studies of Side Chain Effect on the b-1,3-D-Glucan Triple Helix in Aqueous Solution (2008)
  • Author(s): T. Okobira, K. Miyoshi, K. Uezu, K. Sakurai, and S.
  • Journal Title: Biomacromolecules 9 Pages: 783-788
• [Journal Article] Interaction of boric acid with salicyl derivatives as an anchor group of boron-selective adsorbents (2008)
  • Author(s): Y. Miyazaki, H. Matsuo, T. Fujimori, H. Takemura, S. Matsuoka, T. Okobira, K. Uezu, and K. Yoshimura
  • Journal Title: Polyhedron 27 Pages: 2785-2790
• [Journal Article] β-(1→3)-グルカンと核酸とが形づくる3重螺旋構造(連載第7回) (2008)
  • Author(s): 上江洲一也
  • Journal Title: WAKO Infomatic World, January No.8 Pages: 6-7
• [Journal Article] 水中におけるβ-(1→3)-グルカン/核酸複合体の構造解析(連載第8回) (2008)
  • Author(s): 上江洲一也
  • Journal Title: WAKO Infomatic World, February No.9 Pages: 6-7
• [Journal Article] ポリオールへのアンチモン吸着機構(連載第9回、最終回) (2008)
  • Author(s): 上江洲一也
  • Journal Title: WAKO Infomatic World, March No.10 Pages: 5-7
• [Journal Article] Adsorption of bovine serum albumin to a polymer brush prepared by atom-transfer radical polymerization in a porous inorganic membrane (2007)
  • Author(s): H. Kawakita, H. Masunaga, K. Nomura, K. Uezu, I. Akiba, S. Tsuneda
  • Journal Title: Journal of Porous Materials 14(4) Pages: 387-391
• [Journal Article] Sugo,Design of polymer brushes for immobilizing enzymes onto hollow fiber micropores in organic media reaction (2007)
  • Author(s): M. Goto, T. Okubo, H. Kawakita, K. Uezu, S. Tsuneda, K. Saito, M. Goto, M. Tamada and T.
  • Journal Title: Biochem. Eng. J. 37 Pages: 159-165
• [Journal Article] シリーズ解説「イオン交換と計算化学」第5回 (2007)
  • Author(s): 上江洲一也
  • Journal Title: 金属錯体の構造解析と安定性評価 イオン交換学会誌 18巻1号 Pages: 14-20
• [Journal Article] シリーズ解説「イオン交換と計算化学」第6回(最終回) (2007)
  • Author(s): 上江洲一也, 大河平紀司
  • Journal Title: 多糖と核酸との複合体の構造解析 イオン交換学会誌 18巻2号 Pages: 75-82
• [Journal Article] Computational Chemistry in Solvent Extraction (2007)
  • Author(s): K. Uezu and K. Yoshizuka
  • Journal Title: Solvent Extraction Research and Development, Japan, 14 Pages: 1-15
• [Journal Article] 受賞論文(平成18年度日本イオン交換学会進歩賞) (2007)
  • Author(s): 上江洲一也
  • Journal Title: 分子認識部位の設計とイオン交換機構を解析 イオン交換学会誌 18巻3号 Pages: 100-109
• [Journal Article] 思考のツールとしての計算化学(連載第1回) (2007)
  • Author(s): 上江洲一也
  • Journal Title: WAKO Infomatic World, June No.1 Pages: 2-4
• [Journal Article] 新しいβ-(1→3)-グルカン内水素結合の発見(連載第2回) (2007)
  • Author(s): 上江洲一也
  • Journal Title: WAKO Infomatic World, August No.3 Pages: 5-6
• [Journal Article] Al(III)とIn(III)の分離のための□-ジケトン誘導体の分子設計(連載第3回) (2007)
  • Author(s): 上江洲一也
  • Journal Title: WAKO Infomatic World, September No.4 Pages: 6-7
• [Journal Article] カリックスアレーン誘導体によるアルカリ金属イオンの抽出挙動(連載第4回) (2007)
  • Author(s): 上江洲一也
  • Journal Title: WAKO Infomatic World, October No.5 Pages: 5-7
• [Journal Article] 分子集合体のシミュレーション(連載第5回) (2007)
  • Author(s): 上江洲一也
  • Journal Title: WAKO Infomatic World, November No.6 Pages: 6-7
• [Journal Article] 水中におけるβ-(1→3)-グルカンの構造解析(連載第6回) (2007)
  • Author(s): 上江洲一也
  • Journal Title: WAKO Infomatic World, December No.7 Pages: 5-7
• [Journal Article] Preparation of Zr(IV)-immobilized resin prepared by surface template polymerization to remove fluoride ion
  • Author(s): H. Mizuki, H. Kawakita, S. Samatya, K. Uezu
  • Journal Title: Submitted to Separation science and technology
• [Journal Article] The effect of polystyrene addition in Zr(IV)-immobilized resin prepared by surface template polymerization to remove fluoride ion
  • Author(s): S. Samatya, H. Mizuki, H. Kawakita, K. Uezu
  • Journal Title: Submitted to Reactive and Functional Polymers
• [Presentation] Specific capture of phosphoprotein by surface-templated resin (2009)
  • Author(s): H. Mizuki, T. Kawano, K. Uezu
  • Organizer: 2nd Kyushu-Cranfield Joint Colloquium on Plant Cell Biology & Engineering
  • Place of Presentation: Kitakyushu, Japan
  • Year and Date: 20090300
• [Presentation] Immobilized zirconium ion affinity chromatography for specific enrichment of phosphoproteins (2008)
  • Author(s): H. Mizuki, T. Kawano, K. Uezu
  • Organizer: The 3rd Japan-Taiwan Joint International Symposium on Environmental Science and Technology : Environmental Chemistry, Bioscience and Managements
  • Place of Presentation: Kitakyushu, Japan
  • Year and Date: 20081200
• [Presentation] High-speed Removal of Fluorine Ion by Perfusion / Surface-Molecular-Imprinted Resin (2008)
  • Author(s): H. Mizuki, T. Kawano, K. Uezu
  • Organizer: The 21^<th> Symposium on CHEMICAL ENGINEERING
  • Place of Presentation: Saga, Japan
  • Year and Date: 20081200
• [Presentation] Immobilized zirconium ion affinity chromatography for specific enrichment of phosphoproteins (2008)
  • Author(s): H. Mizuki, T. Kawano, K. Uezu
  • Organizer: The first Japan-Korea joint symposium on bio-microsensing technology
  • Place of Presentation: Kitakyushu, Japan
  • Year and Date: 20081100
• [Presentation] 界面鋳型重合法を用いたIMAC用樹脂によるリン酸化タンパク質の分離・精製 (2008)
  • Author(s): 水城秀信, 河野智謙, 上江洲一也
  • Organizer: 化学工学会東北大会
  • Place of Presentation: 仙台
  • Year and Date: 20081000
• [Presentation] Immobilized Zr(IV) affinity chromatography prepared by surface template polymerization for specific capturing phosphorylated proteins (2008)
  • Author(s): H. Mizuki, T. Kawano, K. Uezu
  • Organizer: The Fifth International Workshop on Molecular Imprinting
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20080800
• [Presentation] Specific capture of phosphoprotein by immobilized Zirconium ion affinity chromatography (2008)
  • Author(s): H. Mizuki, T. Kawano, K. Uezu
  • Organizer: 4th International Symposium on Plant Neurobiology
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20080600