• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2009 Fiscal Year Final Research Report

Factors affecting sex-ratio variation and sex-determining mechanisms in the apple snail Pomacea canaliculata

Research Project

  • PDF
Project/Area Number 19570019
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionNara Women's University

Principal Investigator

YUSA Yoichi  Nara Women's University, 理学部, 准教授 (60355641)

Co-Investigator(Kenkyū-buntansha) IWAGUCHI Shin-ichi  奈良女子大学, 理学部, 准教授 (40263420)
Project Period (FY) 2007 – 2009
Keywords生態学 / 進化 / 遺伝学 / 性決定 / 性比 / 軟体動物
Research Abstract

The freshwater snail Pomacea canaliculata shows a large variation in offspring sex ratio. We investigated whether this variation is caused by a small number of sex-determining genes, with two series of experiments. First, we reared F2 offspring from several crosses involving full-sib sisters with the same male, and determined sex ratios of F2. The sex ratios of F2 converged into a few values, indicating that the sex-determining genotypes of the F1 full-sib sisters were also a few. This suggests that the sex in this snail is determined by a few genes rather than polygenes. Secondly, we developed genetic markers to study linkage with the putative sex-determining genes. We found >40 microsatellite loci, and developed four useful markers. These markers were repeatable in PCR, polymorphic and followed Mendelian segregation. We conducted fragment analyses in two populations (Kumamoto and Nara) with these markers. One of them showed significantly different frequencies between males and females in both populations ; it may be in the same linkage group as a sex-determining locus.

  • Research Products

    (16 results)

All 2010 2009 2008 2007

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (8 results)

  • [Journal Article] Learned predator recognition in a freshwater snail2010

    • Author(s)
      Aizaki, K., Yusa, Y.
    • Journal Title

      Pomacea canaliculata. Malacologia 52

      Pages: 21-29

    • Peer Reviewed
  • [Journal Article] 西南暖地において水田から用水路に流出するスクミリンゴガイの個体数2009

    • Author(s)
      和田節, 松倉啓一郎, 吉田和弘, 山西陽子, 遊佐陽一
    • Journal Title

      九州病害虫研究会報 55

      Pages: 93-98

    • Peer Reviewed
  • [Journal Article] Life cycle of the apple snail Pomacea canaliculata (Caenogastropoda: Ampullariidae) inhabiting Japanese paddy fields2009

    • Author(s)
      Yoshida, K., Hoshikawa, K, Wada, T., Yusa, Y.
    • Journal Title

      Applied Entomology and Zoology 44

      Pages: 465-474

    • Peer Reviewed
  • [Journal Article] 奈良八重桜から分離した花酵母でつくった爽やかな旨味の清酒2009

    • Author(s)
      岩口伸一、鈴木孝仁、松澤一幸、清水浩美、大橋正孝、都築正男、藤野千代
    • Journal Title

      生物工学 87

      Pages: 356-357

    • Peer Reviewed
  • [Journal Article] Field observations of the alarm response to crushed conspecifics in the freshwater snail Pomacea canaliculata: effects of habitat, vegetation, and body size2009

    • Author(s)
      Aizaki, K., Yusa, Y.
    • Journal Title

      Journal of Ethology 27

      Pages: 175-180

    • Peer Reviewed
  • [Journal Article] The loss of parts of chromosome 7 followed by the insertion of URA cassette into RB2 on MRS in Candida albicans strain CAI-4.2008

    • Author(s)
      Iwaguchi, S., Suzuki, M., Sakai, N., Yokoyama, K., Suzuki, T.
    • Journal Title

      Medical Mycology 46

      Pages: 655-663

    • Peer Reviewed
  • [Journal Article] Causes of variation in sex ratio and modes of sex determination in the Mollusca-an overview2007

    • Author(s)
      Yusa, Y.
    • Journal Title

      American Malacological Bulletin 23

      Pages: 89-98

    • Peer Reviewed
  • [Journal Article] A water-borne sex pheromone and trail following in the apple snail, Pomacea canaliculata2007

    • Author(s)
      Takeichi, M., Hirai, Y., Yusa, Y.
    • Journal Title

      Journal of Molluscan Studies 73

      Pages: 275-278

    • Peer Reviewed
  • [Presentation] 捕食者種によるスクミリンゴガイの逃避行動の違い2010

    • Author(s)
      上島慧里子, 遊佐陽一
    • Organizer
      第57回日本生態学会大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-17
  • [Presentation] 生物的抵抗性を利用したスクミリンゴガイ広域的管理の可能性2010

    • Author(s)
      遊佐陽一, 原明子, 吉田和弘
    • Organizer
      農研機構シンポジウム「水田農業の新たな展開と技術」
    • Place of Presentation
      東京
    • Year and Date
      2010-03-15
  • [Presentation] Sperm competition among dwarf males of Scalpellum stearnsii (Cirripedia: Lepadomorpha) as revealed by microsatellite markers2009

    • Author(s)
      Ozaki, Y., Iwaguchi, S., Yusa, Y.
    • Organizer
      The Crustacean Society Summer Meeting
    • Place of Presentation
      東京
    • Year and Date
      2009-09-22
  • [Presentation] Ecological roles of microbial volatile organic compounds (MVOCs) in Aspergillus fumigatus2009

    • Author(s)
      Iwaguchi, S., Takaeuchi, T., Kiuchi, M., Okubo, M., Hosokawa, Y., Matsutani, T., Hashimoto, Y., Yokoyama, K., Suzuki, T.
    • Organizer
      The 17th Congress of International Society for Human and Animal Mycology
    • Place of Presentation
      東京
    • Year and Date
      2009-05-29
  • [Presentation] マイクロサテライトマーカーを用いたミョウガガイの矮雄間競争の解明2009

    • Author(s)
      尾崎有紀, 岩口伸一, 遊佐陽一
    • Organizer
      第56回日本生態学会大会
    • Place of Presentation
      盛岡
    • Year and Date
      2009-03-19
  • [Presentation] 水田生態系におけるスクミリンゴガイを介した間接効果2008

    • Author(s)
      吉江春子, 遊佐陽一
    • Organizer
      第55回日本生態学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2008-03-16
  • [Presentation] Sexual selection and sex allocation in Aplysia: hermaphrodites with nonreciprocal mating2007

    • Author(s)
      Yusa, Y.
    • Organizer
      World Congress on Malacology
    • Place of Presentation
      Antwerp, Belgium
    • Year and Date
      20070715-20070720
  • [Presentation] 植物から得られた真菌のInternal transcribed spacer regions (ITS1, ITS2)による検出と同定2007

    • Author(s)
      岩口伸一, 鈴木孝仁
    • Organizer
      第71回日本植物学会年会
    • Place of Presentation
      千葉県野田市
    • Year and Date
      2007-09-07

URL: 

Published: 2011-06-18   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi