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2010 Fiscal Year Final Research Report

Characterization and structural analysis of the plant specific kinesin encoded on the rice genome

Research Project

  • PDF
Project/Area Number 19570047
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionSoka University

Principal Investigator

MARUTA Shinsaku  Soka University, 工学部, 教授 (40231732)

Project Period (FY) 2007 – 2010
Keywordsイネ / 分子モーター / キネシン
Research Abstract

We have succeeded to express several plant specific kinesins encoded on the rice genome using E.coli. expression system. Enzymatic characterization and the crystal structure of the expressed kinesins were analyzed. The results revealed that some of the kinesin have unique enzymatic properties and structure that may reflect the plant specific physiological role of the kinesins.

  • Research Products

    (27 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (23 results) Remarks (1 results)

  • [Journal Article] Characterization of a novel rice kinesin O12 with a calponin homology domain2011

    • Author(s)
      Umezu N., Umeki N., Mitsui T., Kondo K., Maruta S.
    • Journal Title

      Journal of Biochemistry. 149

      Pages: 91-101

    • Peer Reviewed
  • [Journal Article] Biochemical characterization of the novel rice kinesin K23 and its kinetic study using fluorescence resonance energy transfer between an intrinsic tryptophan residue and a fluorescent ATP analogue2011

    • Author(s)
      Umezu N., Hanzawa N., Yamada M.D., Kondo K., Maruta S.
    • Journal Title

      Journal of Biochemistry 149

      Pages: 539-550

  • [Journal Article] Crystallographic analysis reveals a unique conformation of the ADP-bound novel rice kinesin K162010

    • Author(s)
      Tanaka K., Umeki N., Mitsui T., Fujimoto Z., Maruta S.
    • Journal Title

      Biochem Biophys Res Commun. 401

      Pages: 251-256

    • Peer Reviewed
  • [Presentation] Preparation and polymerization of tubulin of ginkgo biloba and its interaction with rice plant kinesin2011

    • Author(s)
      Iwata S., Umezu N., Kondo K., Maruta S.
    • Organizer
      第55回米国生物物理学会
    • Place of Presentation
      Baltimore, MD USA.
    • Year and Date
      2011-03-08
  • [Presentation] Characterization of the plant-specific rice kinesins2011

    • Author(s)
      Umezu N., Umeki N., Hanzawa N., Kondo K., Mitsui T., Maruta S.
    • Organizer
      第55回米国生物物理学会
    • Place of Presentation
      Baltimore, MD USA.
    • Year and Date
      2011-03-06
  • [Presentation] The characterization of the novel rice kinesin K23 at kinesin-7 subfamily2010

    • Author(s)
      Nozomi Umezu, Nobue Hanzawa, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta
    • Organizer
      第83回日本生化学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
  • [Presentation] Kinetic studies on the novel rice kinesin K23 utilizing FRET between the intrinsic tryptophan residue and fluorescent ATP analogue2010

    • Author(s)
      Nozomi Umezu, Nobue Hanzawa, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-21
  • [Presentation] Preparation of microtubules derived from plant and its interaction with rice plant kinesin2010

    • Author(s)
      Seigo Iwata, Kazunori Kondo, Shinsaku Maruta
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-21
  • [Presentation] ANALYSIS OF THE ROLE OF UNIQUE LOOP L5 IN RICE KINESIN K16 MOTOR DOMAIN2010

    • Author(s)
      Ishikawa K., Tanaka K., Maruta S.
    • Organizer
      第54回米国生物物理学会
    • Place of Presentation
      San Francisco, CA USA.
    • Year and Date
      2010-02-21
  • [Presentation] Expression and characterization of novel rice kinesin E15.2009

    • Author(s)
      Masako TSUCHIDA, Masafumi D.YAMADA, Kazunori KONDO, 丸田晋策
    • Organizer
      Biophysical Society 53^<rd> Annual Meeting(Biophysical Journal Abstracts Issue : p.137a)
    • Place of Presentation
      Boston USA
    • Year and Date
      20090200
  • [Presentation] Kinetic characterization of the rice kinesins using fluorescent-ATP analogue.2009

    • Author(s)
      Nozomi UMEZU, Yuko KUBO, Kazunori KONDO, Toshiaki MITSUI, Shinsaku MARUTA
    • Organizer
      Biophysical Society 53^<rd> Annual Meeting(Biophysical Journal Abstracts Issue : p.137a)
    • Place of Presentation
      Boston USA
    • Year and Date
      20090200
  • [Presentation] Analysis of crystal structure and solution structure of the motor domain of rice kinesin K16.2009

    • Author(s)
      Shinsaku MARUTA, Keiko TANAKA, Yoko KUBO, Toshiaki MITSUI, Zui FUJIMOTO
    • Organizer
      Biophysical Society 53^<rd> Annual Meeting(Biophysical Journal Abstracts Issue : p.137a)
    • Place of Presentation
      Boston USA
    • Year and Date
      20090200
  • [Presentation] Analysis fo conformational change of novel rice kinesin K16 using small angle X-ray solution scattering and EPR.2009

    • Author(s)
      Yuko KUBO, Satoshi YASUDA, Yasunobu SUGIMOTO, Masafumi YAMADA, Toshiaki ARATA, Katsuzo WAKABAYASHI, Shinsaku MARUTA
    • Organizer
      Biophysical Society 53^<rd> Annual Meeting(Biophysical Journal Abstracts Issue : p.138a)
    • Place of Presentation
      Boston USA
    • Year and Date
      20090200
  • [Presentation] Characterization of the Novel Rice Kinesin O12 which Has CH Domain2009

    • Author(s)
      Umezu N., Kondo K., Mitsui T., Maruta S.
    • Organizer
      第49回米国細胞生物学会年会
    • Place of Presentation
      San Diego, CA USA.
    • Year and Date
      2009-12-08
  • [Presentation] Characterization of the novel rice kinesin K232009

    • Author(s)
      Nobue Hanzawa, Nozomi Umezu, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      徳島
    • Year and Date
      2009-10-31
  • [Presentation] Characterization of the plant rice kinesin O12 that has a calponin homology domain2009

    • Author(s)
      Nozomi Umezu, Kazunori Kondo, Toshiaki Mitsui, Shinsaku Maruta
    • Organizer
      第82回日本生化学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22
  • [Presentation] Kinetic characterization of the novel rice kinesin L05 and L04 using fluorescent ATP analogue.2008

    • Author(s)
      Nozomi UMEZU, Yuko KUBO, Toshiaki MITSUI, Shinsaku MARUTA
    • Organizer
      第46回日本生物物理学会(同学会要旨集 p.S101)
    • Place of Presentation
      福岡
    • Year and Date
      20081200
  • [Presentation] Analysis of crystal structure of the motor domain of rice kinesin K16 and comparison with the structures of other related kinesin.2008

    • Author(s)
      Keiko TANAKA, Toshiaki MITSUI, Zui FUJIMOTO, Shinsaku MARUTA
    • Organizer
      第46回日本生物物理学会(同学会要旨集 p.S102)
    • Place of Presentation
      福岡
    • Year and Date
      20081200
  • [Presentation] Preparation and characterization of novel rice kinesin E15.2008

    • Author(s)
      Masako TSUCHIDA, Masafumi D. YAMADA, Kazunori KONDO, Shinsaku MARUTA
    • Organizer
      第46回日本生物物理学会(同学会要旨集 p.S102)
    • Place of Presentation
      福岡
    • Year and Date
      20081200
  • [Presentation] Analysis fo conformational change of novel rice kinesin K16 using small angle X-ray scattering and FRET.2008

    • Author(s)
      Yuko KUBO, Masafumi D.YAMADA, Hideki SHISHIDO, Yasunobu SUGIMOTO, Katsuzo WAKABAYASHI, Shinsaku MARUTA
    • Organizer
      第46回日本生物物理学会(同学会要旨集 p.S103)
    • Place of Presentation
      福岡
    • Year and Date
      20081200
  • [Presentation] Analysis of conformational change of novel rice kinesin K16 using small angle X-ray solution scattering.2008

    • Author(s)
      Yuko KUBO, Masafumi D.YAMADA, Yasunobu SUGIMOTO, Katsuzo WAKABAYASHI, Shinsaku MARUTA
    • Organizer
      2^<nd> International symposium on Bio-nanosystems(Abstract : p.67)
    • Place of Presentation
      Tokyo
    • Year and Date
      20081100
  • [Presentation] X線小角散乱を用いた新規イネキネシンK16の構造変化の解析2007

    • Author(s)
      久保優子、山田正文、杉本泰伸、若林克三、丸田晋策
    • Organizer
      第45回日本生物物理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-22
  • [Presentation] 結晶構造解析で明らかになったイネキネシンK16のネックリンカーの特徴的コンホメーション2007

    • Author(s)
      丸田晋策、梅木伸久、三ツ井敏明、藤本瑞
    • Organizer
      第45回日本生物物理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-22
  • [Presentation] 蛍光標識ATPアナログを用いた新規イネキネシンのK16のATPase kinetic pathwayの観察2007

    • Author(s)
      土田雅子、山田正文、吉村栄子、近藤和典、丸田晋策
    • Organizer
      第45回日本生物物理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-22
  • [Presentation] 蛍光標識ATPアナログを用いたイネキネシン012の生化学的解析2007

    • Author(s)
      梅津のぞみ、梅木伸久、近藤和典、三ツ井敏明、丸田晋策
    • Organizer
      第45回日本生物物理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-22
  • [Presentation] イネキネシンK16モータードメインの結晶構造2007

    • Author(s)
      丸田晋策、梅木伸久、三ツ井敏明、藤本瑞
    • Organizer
      第80回日本生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
  • [Remarks] ホームページ

    • URL

      http://www.t.soka.ac.jp/maruta-lab/

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Published: 2012-01-26   Modified: 2016-04-21  

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