2009 Fiscal Year Final Research Report
Structural Studies on precursor proteins of bioactive peptides to elucidate their maturation.
| Project/Area Number |
19570112
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kwansei Gakuin University |
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Principal Investigator |
YAMAGUCHI Hiroshi Kwansei Gakuin University, 理工学部, 教授 (10252719)
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| Co-Investigator(Kenkyū-buntansha) |
HIDAKA Yuji 近畿大学, 理工学部, 准教授 (70212165)
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| Project Period (FY) |
2007 – 2009
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| Keywords | X線結晶構造解析 / 結晶構造 / フォールディング解析 / フォールディング中間体 / ウログアニリン / プロウログアニリン / アミロイド前駆体タンパク質 |
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| Research Abstract |
Prouroguanylin (PUG) is precursor of uroguanylin that is peptide hormone. X-ray crystal structure of PUG has been determined at high resolution. In the folding process of PUG, the structure suggests that not only interactions between proregion and peptide region in the PUG that has correct folding but also those in the folding intermediates are very important. Furthermore, kinetic analysis of a folding process of PUG has been performed. It is elucidated that two folding intermediate structures are important in the folding process. In addition, behaviors of β-amyloid precursor protein in the solution were analyzed by CD, DSC and light scattering.
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