2009 Fiscal Year Final Research Report
Establishment of hierarchical structure analysis of proteins insolutions by high-throughput of wide-angle X-rays scattering usingsynchrotron radiation source
| Project/Area Number |
19570148
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
HIRAI Mitsuhiro Gunma University, 大学院・工学研究科, 教授 (00189820)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Keywords | タンパク質 / 生体膜 / 階層構造 / フォールディング / アミロイド形成 / X線広角散乱 / 放射光 |
|---|
| Research Abstract |
The wide-angle X-ray scattering using the third generation synchrotron radiation source is a promising and powerful method elucidating in-situ hierarchical structures of proteins at those functional states. By the excution of this study aimed for the establishment of the solution X-ray wide-angle scattering, we can get scattering data with high statistics precision while avoiding radiation damage, and we clarified the relation between hydration shell and structural transition of a protein, the all processes of nucleation / growth in the amyloid metastasis, the change of dynamics of a protein induced by the amyloid transition (α-to-cross-β).We published the aobve results of this project in 11 full articles in academic journals and in 23 papers at overseas international conferences and at domestic academic society meetings.
|
