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2008 Fiscal Year Final Research Report

Study of the dynamic structure of the troponin-tropomyosin complex

Research Project

  • PDF
Project/Area Number 19570152
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionUniversity of Fukui

Principal Investigator

三木 正雄  University of Fukui, 工学研究科, 教授 (30242580)

Project Period (FY) 2007 – 2008
Keywordsタンパク質 / 核酸の構造 / 動態 / 機能
Research Abstract

トロポミオシンやトロポニン複合体(TnC, TnI, TnT)の遺伝子操作により、おのおの、シングルシステイン残基をもつ変異体を作り出して、そのシステイン残基それぞれに蛍光色素をラベルして、蛍光測定を行うことにより、トロポミオシンートロポニン、トロポミオシンーアクチン、トロポニンートロポミオシンの相互作用について明らかにした。

  • Research Products

    (9 results)

All 2009 2008 2007 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (5 results) Book (1 results)

  • [Journal Article] Fluorescence Resonance Energy Transfer between Residues on Troponin and Tropomyosin in the Reconstituted Thin Filament : Modeling the Troponin-Tropomyosin Complex Kimura-Sakiyama2008

    • Author(s)
      C., Ueno, Y., Wakabayashi, K., and Miki, M.
    • Journal Title

      J.Mol.Biol 376

      Pages: 80-91

    • Peer Reviewed
  • [Journal Article] Conformational Changes in Reconstituted Skeletal Muscle Thin Filaments Observed By Fluorescence Spectroscopy2007

    • Author(s)
      Miki, M
    • Journal Title

      Adv.Exp.Med. Biol 592

      Pages: 111-123

    • Peer Reviewed
  • [Journal Article] Fluorescence emission from PAMAM and PPI dendrimers

    • Author(s)
      Wang, D., Imae, T., and Miki, M
    • Journal Title

      Journal of Colloid and Interface Science

  • [Presentation] 再構成細いフィラメント上でのアクチンートロポミオシン残基間の蛍光エネルギー移動測定 : アクチ ンートロポミオシン複合体モデル2009

    • Author(s)
      三木正雄, 斎藤隆広、飛田英孝、上野豊、木邑智恵子
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      東京大学数理科学研究科
    • Year and Date
      2009-01-10
  • [Presentation] FRET between Residues on Tropomyosin and Actin in the Reconstituted Thin Filament : Modeling the Actin-Tropomyosin Complex2008

    • Author(s)
      T. Saitoh, Y. Ueno, C. Kimura, and M. Miki
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
  • [Presentation] 筋肉細いフィラメント上のトロポミオシンートロポニンの構造変化2008

    • Author(s)
      Miki, M
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      仙台市戦災復興記念館 記念ホール
    • Year and Date
      2008-01-07
  • [Presentation] Study of the dynamic structure of the tropomyosin molecule in muscle thin filaments by fluorescence quenching measurements2007

    • Author(s)
      Takahiro Saitoh, Yousuke Matsuda, and Masao Miki
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-22
  • [Presentation] Applications of fluorescence resonance energy transfer to muscle proteins.

    • Author(s)
      Miki, M
    • Organizer
      The 7^<th> KIAS-Soogsil Conference on protein structure and Function
    • Place of Presentation
      Soongsil University, Seoul, Korea(招待講演)
  • [Book] 生物物理学ハンドブック(石渡信一他編集)

    • Author(s)
      三木正雄
    • Publisher
      朝倉書店

URL: 

Published: 2010-06-10   Modified: 2016-04-21  

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