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2008 Fiscal Year Final Research Report

Structure and function relationships of the anti -oncogenic START-GAP family gene products

Research Project

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Project/Area Number 19570184
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionUniversity of Hyogo

Principal Investigator

HITOSHI Yagisawa  University of Hyogo, 大学院・生命理学研究科, 准教授 (40192380)

Project Period (FY) 2007 – 2008
Keywordsシグナル伝達 / 細胞骨格・運動 / 抗がん遺伝子
Research Abstract

哺乳動物には、STARTドメインを持つRhoファミリー低分子量Gタンパク質に対するGTPアーゼ活性化タンパク質(START-GAP)をコードする遺伝子が3つ存在し、これらの遺伝子産物の抗腫瘍活性が注目されている。それぞれの生化学・細胞生物学的性質を比較解析したところ、START-GAPの接着斑への局在化が細胞運動性や形態維持に重要な役割を果たしていることが判明した。また、局在化に必要な領域を同定し、その分子機構の一部を解明した。

  • Research Products

    (25 results)

All 2009 2008 2007 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (16 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Dynamin 2 is required for actin assembly in phagocytosis in Sertoli cells2009

    • Author(s)
      Otsuka, A., Abe, T., Watanabe, M., Yagisawa, H., Takei, K. and Yamada, H.
    • Journal Title

      Biochem Biophys Res Commun 378

      Pages: 478-482

    • Peer Reviewed
  • [Journal Article] Focal adhesion-localization of START-GAP1/DLC1 is essential for cell motility and morphology2009

    • Author(s)
      Kawai, K., Iwamae, Y., Yamaga, M., Ishii, H., Hirata, H., Homma, Y. and Yagisawa, H.
    • Journal Title

      Genes Cells 14

      Pages: 227-241

    • Peer Reviewed
  • [Journal Article] START-GAP2/DLC2 is localized in focal adhesions via its N-terminal region2009

    • Author(s)
      Kawai, K., Seike, J., Iino, T., Kiyota, M., Iwamae, Y. and Yagisawa, H.
    • Journal Title

      Biochem. Biophys. Res. Commun 380

      Pages: 736-741

    • Peer Reviewed
  • [Journal Article] Recruitment and activation of phospholipase C (PLC)-δ_1 in lipid rafts by muscarinic stimulation of PC12 cells : contribution of p122RhoGAP/DLC1, a tumor-suppressing PLCδ_1 binding protein2008

    • Author(s)
      Yamaga, M., Kawai, K., Kiyota, M., Homma Y. and Yagisawa, H.
    • Journal Title

      Adv. Enzyme Regul 48

      Pages: 41-54

    • Peer Reviewed
  • [Journal Article] Phosphatidylserine induces functional and structural alterations of the membrane-associated PH domain of phospholipase C-δ12007

    • Author(s)
      Uekama, N., Sugita, T., Okada, M., Yagisawa, H. and Tuzi, S.
    • Journal Title

      FEBS J. 274

      Pages: 177-187

    • Peer Reviewed
  • [Journal Article] Lipid components in the detergent-resistant membrane microdomain (DRM) obtained from the synaptic plasma membrane of rat brain2007

    • Author(s)
      Matsuura, D., Taguchi, K., Yagisawa, H. and Maekawa S.
    • Journal Title

      Neurosci. Lett. 423

      Pages: 158-161

    • Peer Reviewed
  • [Journal Article] START-GAP3/DLC3 is a GAP for RhoA and Cdc42 and is localized in focal adhesions regulating cell morphology2007

    • Author(s)
      Kawai, K., Kiyota, M., Seike J., Deki, Y. and Yagisawa H.
    • Journal Title

      Biochem. Biophys. Res. Commun 364

      Pages: 783-789

    • Peer Reviewed
  • [Presentation] Influence of membrane curvature on the structure of the phospholipase C (PLC)-δ1 PH domain : implication in intracellular localization2009

    • Author(s)
      Uekama, N., Aoki, T., Muraoka, T., Yagisawa, H., Nishimura K. and Tuzi, S.
    • Organizer
      Gordon Research Conference on "Signal Transduction Within the Nucleus."
    • Place of Presentation
      California, USA
    • Year and Date
      20090329-0703
  • [Presentation] イノシトールリン脂質代謝酵素の核-細胞質間移行とその生理的意義BMB20082008

    • Author(s)
      八木澤仁
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会シンポジウム「核-細胞質間輸送と生命機能制御のインターフェイス」
    • Place of Presentation
      神戸
    • Year and Date
      20081209-12
  • [Presentation] START-GAP1の接着斑局在化におけるテンシン2との結合の役割2008

    • Author(s)
      北村真也、前平航司、川合克久、西谷秀男、八木澤仁
    • Organizer
      BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20081209-12
  • [Presentation] START-GAP1は接着斑局在化領域のテンシン2に対するリン酸化依存的結合により接着斑に局在化する2008

    • Author(s)
      川合克久、北村真也、川合由華、西谷秀男、八木澤仁
    • Organizer
      BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20081209-12
  • [Presentation] 脂質膜上における局所構造の特徴に基づくpleckstrin homology domain-脂質膜間相互作用の解析2008

    • Author(s)
      徳田尚美、八木澤仁、福井泰久、辻暁
    • Organizer
      第47回NMR討論会
    • Place of Presentation
      筑波
    • Year and Date
      20081112-14
  • [Presentation] Ca^<2->-dependent nuclear accumulation of phospholipase C-δ12008

    • Author(s)
      Yagisawa, H. and Okada, M.
    • Organizer
      第31回神経科学会大会
    • Place of Presentation
      東京
    • Year and Date
      20080709-11
  • [Presentation] Measurement of inositol polyphosphates by ion chromatography and their physiological status in higher plants2008

    • Author(s)
      Mimura, T., Mitsuhashi, N., Richardso, A., Chung, S-K. and Yagisawa, H.
    • Organizer
      第31回神経科学会大会
    • Place of Presentation
      東京
    • Year and Date
      20080709-11
  • [Presentation] 固体NMR分光法による膜表在性タンパク質PLC-δ1の局所運動性の解析2007

    • Author(s)
      上釜奈緒子、八木澤仁、辻暁、西村勝之
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      20071221-26
  • [Presentation] ホスホリパーゼC-δ1PHドメインおよびEF-handドメインの脂質二重膜上における構造とダイナミクス2007

    • Author(s)
      三宅健史、川合克久、八木澤仁、辻暁
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      20071221-23
  • [Presentation] 脂質二重膜表面におけるpleckstrin homology domain の動的構造の解析2007

    • Author(s)
      徳田尚美、八木澤仁、福井泰久、辻暁
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      20071221-23
  • [Presentation] 脂質二重膜表面の曲率とホスホリパーゼC-δ1 PH ドメインの立体構造変化 : 固体NMR分光法による解析2007

    • Author(s)
      上釜奈緒子、青木隆広、丸岡俊宏、栗栖聖治、畠山明子、岡田雅司、八木澤仁、西村勝之、辻暁
    • Organizer
      BMB2007(第30回日本分子生物学会年会・第80回日本生化学会合同大会)
    • Place of Presentation
      横浜
    • Year and Date
      20071211-14
  • [Presentation] p122RhoGAPによる細胞形態変化には接着斑への局在化が寄与する2007

    • Author(s)
      川合克久、清田稔、清家淳一、岩前由衣、八木澤仁
    • Organizer
      BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会)
    • Place of Presentation
      横浜
    • Year and Date
      20071211-14
  • [Presentation] Phosphorylation of phospholipase C-δ_1 regulates its enzymatic activity2007

    • Author(s)
      Fujii, M. and Yagisawa, H.
    • Organizer
      Howard Hughes Medical Institute, Janelia Conference on "Inositide Signalling"
    • Place of Presentation
      Virginia USA
    • Year and Date
      20071104-07
  • [Presentation] START-GAP1, a PLCδ_1-binding RhoGAP, in focal adhesions regulates cell morphology and motility2007

    • Author(s)
      Yagisawa, H., Seike, J., and Kawai, K.
    • Organizer
      Howard Hughes Medical Institute, Janelia Conference on "Inositide Signalling"
    • Place of Presentation
      Virginia USA
    • Year and Date
      20071104-07
  • [Presentation] Regulation of intracellular localization of a dual functional PLCδ1-binding protein, p122RhoGAP/DLC1, a member of the tumor suppressing START-GAP family proteins2007

    • Author(s)
      Yagisawa, H., Kawai, K., Iwamae, Y., Kiyota, M. and Yamaga, M.
    • Organizer
      48^<th> International Symposium on "Regulation of Enzyme Activity and Synthesis in Normal and Neoplastic Tissues
    • Place of Presentation
      Bologna, Italy
    • Year and Date
      20070924-25
  • [Presentation] 固体NMRによる膜表在性タンパク質PLC-δ1PHドメインの局所運動性解析2007

    • Author(s)
      上釜奈緒子、八木澤仁、辻暁、西村勝之
    • Organizer
      第46回NMR討論会
    • Place of Presentation
      札幌
    • Year and Date
      20070910-13
  • [Book] Phosphoinositide-specific phospholipase C : Isoforms and related molecules2009

    • Author(s)
      Yagisawa, H.
    • Total Pages
      269-296
    • Publisher
      Berlin Heidelberg(In "Handbook of Neurochemistry and Molecular Neurobiology (3^<rd> Edition), Neural Signal Mechanism Vol. 1b (ed. Mikoshiba K.), Chapter 14 : Phospholipid signaling and cell function, Springer-Verlag)
  • [Remarks]

    • URL

      http://www.sci.u-hyogo.ac.jp/life/biosig/kenkyu.html

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Published: 2010-06-10   Modified: 2016-04-21  

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