Functional analysis of Rab proteins involved in endocytic transport in multicellular organism.

2009 Fiscal Year Final Research Report

• Project/Area Number: 19570186
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: ANDO Keiko Tokyo Women's Medical University, 総合研究機構脳科学融合研究センター, 特任准教授 (40221741)
• Project Period (FY): 2007 – 2009
• Keywords: 小胞輸送 / 線虫 / エンドサイトーシス

Research Abstract

A small G protein, Rab, is a major protein family which controls membrane traffic in eucaryotes. There are more than 60 members of Rab in the human genome, however, the biological functions of these proteins are not well understood. To clarify the physiological roles of Rab proteins in the endocytic transport, we isolated deletion mutants of genes encoding Rabs and their cooperating proteins in C.elegans. We systematically performed endocytosis assays using these mutations and identified proteins involved in endocytic transport in the multicellular organism.

Research Products

• [Journal Article] モデル生物線虫から見た低分子量G蛋白質 Rab (2010)
  • Author(s): 安藤恵子
  • Journal Title: 細胞 42(3) Pages: 104-107
• [Journal Article] IFT-81 and IFT-74 are required for intraflagellar transport in C. elegans (2007)
  • Author(s): Kobayashi, T., Gengyo-Ando, K., Ishihara, T., Katsura, I., Mitani, S.
  • Journal Title: Genes Cells 12 Pages: 593-602
  • Peer Reviewed
• [Journal Article] The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans (2007)
  • Author(s): Gengyo-Ando K, Kuroyanagi H, Kobayashi T, Murate M, Fujimoto K, Okabe S, Mitani S.
  • Journal Title: EMBO Reports 8(2) Pages: 152-7
  • Peer Reviewed
• [Presentation] メンブレントラフィクに関与する線虫Sec1/Munc18ファミリーのゲノム機能解析 (2009)
  • Author(s): 安藤恵子, 中臺枝里子, 三谷昌平
  • Organizer: 第61回日本細胞生物学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 20090602-20090604
• [Presentation] Functional genomics on membrane trafficking in C. elegans (2007)
  • Author(s): Gengyo-Ando, K., Mitani S.
  • Organizer: International Symposium "Gene Expression Control and Genome Evolution"
  • Place of Presentation: Okayama, Japan
  • Year and Date: 20070919-20070921
• [Presentation] Physiological roles of the Sec1/Munc18 family in the endosomal/lysosomal system of C. elegans (2007)
  • Author(s): Gengyo-Ando, K., Kuroyanagi, H., Kobayashi, T., Murate, M., Fujimoto, K., Okabe, S., Mitani, S.
  • Organizer: 16th International C. elegans Meeting
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 20070627-20070701
• [Remarks] ホームページ等
  • URL: http://www.saitama-u.ac.jp/iron/hP-kenkyo/shinkou/nou.htm