2009 Fiscal Year Final Research Report
Structure and function of enzymes involved with the catabolisation of GABA in Bacillus subtilis.
| Project/Area Number |
19579003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Toho University |
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Principal Investigator |
GOTO Masaru Toho University, 理学部, 講師 (80379289)
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| Project Period (FY) |
2007 – 2009
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| Keywords | X線結晶解析 |
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| Research Abstract |
The recombinant succinate semialdehyde dehydrogenase with His-tag was purified in one chromatographic step by using Ni-NTA resin. The structures of GABA aminotransferase have determined at acidic and neutral pH. Crystals of GabR protein was obtained by the hanging-drop vapor-diffusion method. The X-ray diffraction data set for the native crystal was collected to 2.2Å resolution. The phase of native GabR was solved by the multiple isomorphous replacement method, using Hg derivative data sets. Now the structure of native GabR is under construction.
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