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2009 Fiscal Year Final Research Report

Identification of the gene for a sporeless mutation and its application in edible mushroom breeding.

Research Project

  • PDF
Project/Area Number 19580004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionTottori University

Principal Investigator

MATSUMOTO Teruyuki  Tottori University, 農学部, 教授 (60132825)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Shigeyuki  財団法人日本きのこセンター, 菌蕈研究所, 上席主任研究員 (00072794)
Project Period (FY) 2007 – 2009
Keywords食用担子菌類 / 胞子欠損性変異 / 連鎖解析 / AFLPマーカー / 分子育種 / ウスヒラタケ
Research Abstract

In this study, we constructed the genetic linkage map of the sporeless mutant of Pleurotus pulmonarius based on the DNA markers, and determined the locus of sporeless mutation. Moreover, we obtained the DNA markers which are very tightly linked to this locus. These results enable to carry out the efficient breeding of a sporeless mushrooms. In addition, we cloned the region related to this mutation, and based on the information of linkage map we determined the whole nucleotide sequences of this region. By the comparison of the sequences from mutant and wild type, we found the differences in several gene structures. In future, we will identify the gene that causes the sporeless mutation of this fungus by using the complementation test and so on.

  • Research Products

    (10 results)

All 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (7 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] A genetic linkage map of Pleurotus pulmonarius based on AFLP markers, and localization of the gene region for the sporeless mutation2009

    • Author(s)
      Okuda, Y. 、Murakami, S., Matsumoto, T.
    • Journal Title

      Genome 52

      Pages: 483-446

    • Peer Reviewed
  • [Journal Article] Development of STS markers suitable for marker-assisted selection of sporeless trait in oyster mushroom2009

    • Author(s)
      Okuda, Y. 、Murakami, S., Matsumoto, T.
    • Journal Title

      Pleurotus pulmonarius、Breeding Science Vol.59

      Pages: 315-319

    • Peer Reviewed
  • [Presentation] リアルタイムpcr法を用いたウスヒラタケ無胞子形質の迅速選抜法2010

    • Author(s)
      奥田康仁・村上重幸・松本晃幸
    • Organizer
      日本育種学会第117回講演会
    • Place of Presentation
      京都府京都市、京都大学
    • Year and Date
      20100326-20100327
  • [Presentation] ウスヒラタケ(Pleurotus pulmonarius)胞子欠損性変異関連領域のマップベースクローニング2009

    • Author(s)
      奥田康仁・村上重幸・松本晃幸
    • Organizer
      日本菌学会第53回大会
    • Place of Presentation
      鳥取県鳥取市、鳥取大学
    • Year and Date
      20090820-20090821
  • [Presentation] Genetic linkage map and STS markers for breeding of a sporeless strain in oyster mushroom, Pleurotus pulmonarius2009

    • Author(s)
      Okuda, Y.、Murakami, S., Matsumoto, T.
    • Organizer
      25th Fungal Genetics Conference at Asilomar
    • Place of Presentation
      Asilomar、California、USA
    • Year and Date
      20090317-20090322
  • [Presentation] ウスヒラタケ(Pleurotus pulmonarius)における胞子欠損性変異検出STSマーカーの開発2008

    • Author(s)
      奥田康仁・村上重幸・松本晃幸
    • Organizer
      第8回糸状菌分子生物学コンファレンス
    • Place of Presentation
      石川県金沢市、石川県文教会館
    • Year and Date
      20081117-20081118
  • [Presentation] Identification of markers closely linked to the gene region for sporeless mutation in Pleurotus pulmonarius by bulked segregant analysis2008

    • Author(s)
      Okuda, Y.、Murakami, S., Matsumoto, T.
    • Organizer
      The 5th Meeting of East Asia for Collaboration on Edible Fungi
    • Place of Presentation
      福岡県福岡市、九州大学国際研究交流プラザ
    • Year and Date
      20080916-20080917
  • [Presentation] AFLPマーカーに基づくウスヒラタケ(Pleurotus pulmonarius)の遺伝連鎖地図および胞子欠損性変異領域の座乗部位2008

    • Author(s)
      奥田康仁・村上重幸・松本晃幸
    • Organizer
      日本菌学会第52回大会
    • Place of Presentation
      三重県津市、三重大学
    • Year and Date
      20080530-20080601
  • [Presentation] Bulked segregant analysisによるウスヒラタケ(Pleurotus pulmonarius)の胞子欠損性変異領域近傍マーカーの同定2008

    • Author(s)
      奥田康仁・村上重幸・松本晃幸
    • Organizer
      日本きのこ学会第12回大会
    • Place of Presentation
      福岡県福岡市、九州大学医学部
    • Year and Date
      2008-09-16
  • [Patent(Industrial Property Rights)] 胞子欠損性変異検出マーカー2009

    • Inventor(s)
      松本晃幸・村上重幸・奥田康仁
    • Industrial Property Rights Holder
      国立大学法人鳥取大学
    • Industrial Property Number
      特願2009-054317
    • Filing Date
      2009-03-08

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Published: 2011-06-18   Modified: 2016-04-21  

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