Gain of plant disease resistance by plant cell homeostasis and anti-microbial second metabolite

2009 Fiscal Year Final Research Report

• Project/Area Number: 19580047
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Okayama University
• Principal Investigator: INAGAKI Yoshishige Okayama University, 大学院・自然科学研究科, 准教授 (50280764)
• Co-Investigator(Renkei-kenkyūsha): OSBOURN Anne ジョンイネスセンター, 植物代謝部門, 部門長/教授
• Project Period (FY): 2007 – 2009
• Keywords: 感染生理 / 非宿主抵抗性 / 過敏感反応 / Cdc27B遺伝子 / 抗菌性二次代謝産物 / Oxidosqualene cyclase / P450 sterol demethylase遺伝子 / トリテルペンサポニン

Research Abstract

In this study, we tried to make sense the mechanism of novel plant disease resistance unlike to conventional plant disease resistance, that are host-specific resistance and non-host resistance, using not only plant pathology, but also plant cell physiology and plant molecular biology. A couple of the novel plant disease resistance is 1) plant cell homeostasis, that is the function for keeping natural state of plant cell going, and 2) the faculty for biosynthesis of anti-microbial second metabolite for plant defense. We have revealed that Cdc27B as cell cycle regulator are involved in plant disease resistance in tobacco, and the genes for triterpene-saponin biosynthesis in rice.

Research Products

• [Journal Article] The siderophore pyoverdine of Pseudomonas syringae pv. tabaci 6605 is an intrinsic virulence factor in host tobacco infection. (2010)
  • Author(s): Taguchi F, Suzuki T, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y
  • Journal Title: J. Bacteriol 192 Pages: 117-126
• [Journal Article] Glycosylation of flagellin from Pseudomonas syringae pv. tabaci 6605 contributes to evasion of host tobacco plant surveillance system. (2009)
  • Author(s): Taguchi F, Suzuki T, Takeuchi K, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y
  • Journal Title: Physiol. Mol. Plant Pathol 74 Pages: 11-17
• [Journal Article] Bacterial DNA activates immunity in Arabidopsis thaliana. (2009)
  • Author(s): Yakushiji S, Ishiga Y, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y
  • Journal Title: J. Gen. Plant Pathol 75 Pages: 227-234
• [Journal Article] Amino acid sequence of bacterial microbe-associated molecular pattern flg22 is required for virulence. (2008)
  • Author(s): Naito K, Taguchi F, Suzuki T, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y
  • Journal Title: Mol Plant-Microbe Interact 21(9) Pages: 1165-1174
• [Journal Article] Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana. (2008)
  • Author(s): Higashi, K., Ishiga, Y., Inagaki, Y., Toyoda, K., Shiraishi, T., Ichinose, Y
  • Journal Title: Mol. Genet. Genomics 279(3) Pages: 303-312
• [Journal Article] Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction. (2008)
  • Author(s): Marutani, M., Taguchi, F., Ogawa, Y., Hossain, M.M., Inagaki, Y., Toyoda, K., Shiraishi, T., Ichinose, Y
  • Journal Title: Mol. Genet. Genomics 279(4) Pages: 313-322
• [Journal Article] Elicitin-responsive lectin-like receptor kinase in BY-2 cells. (2007)
  • Author(s): Sasabe, M., Naitoh, K., Suenaga, H., Ikeda, T., Toyoda, K., Inagaki, Y., Shiraishi, T., Ichinose, Y
  • Journal Title: DNA Sequence 18 Pages: 152-159
• [Journal Article] Suppression of Cdc27B induces plant defense responses. (2007)
  • Author(s): Kudo C, Suzuki T, Fukuoka S, Asai S, Suenaga H, Sasabe M, Takano Y, Okuno T, Toyoda K, Shiraishi T, Ichinose Y, Inagaki Y
  • Journal Title: Mol Plant Pathol 8(4) Pages: 365-373
• [Presentation] Anti-microbal activity of avenacin to fungal pathogens for cereals. (2009)
  • Author(s): Inagaki Y, Imaoka A, Fujita K, Arase S, Osbourn A, Toyoda K, Shiraishi T, Ichinose Y
  • Organizer: The 1st KSPP PSJ Joint meeting
  • Place of Presentation: Jeju, Korea
  • Year and Date: 20091028-20091031
• [Presentation] Avenacin saponin has anti-microbal activity to fungal pathogens for cereals. (2009)
  • Author(s): Inagaki Y, Imaoka A, Fujita K, Arase S, Osbourn A, Toyoda K, Shiraishi T, Ichinose Y
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Quebec, Canada
  • Year and Date: 20090719-20090723
• [Presentation] Fungicidal activity of avenacin to fungal pathogens for cereals. (2009)
  • Author(s): Inagaki Y, Imaoka A, Fujita K, Arase S, Osbourn A, Toyoda K, Shiraishi T, Ichinose Y
  • Organizer: Terpnet 2009
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20090525-20090529
• [Presentation] イネCYP51 sterol demethylase遺伝子についての分子解析 (2009)
  • Author(s): 稲垣善茂・豊田和弘・白石友紀・一瀬勇規・Anne Osbourn
  • Organizer: 第50回日本植物生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 20090321-20090324
• [Presentation] サポニン生合成に関与するイネCYP51 sterol demethylase遺伝子の分子解析 (2008)
  • Author(s): 稲垣善茂・豊田和弘・白石友紀・一瀬勇規・Anne Osbourn
  • Organizer: 平成20年度日本植物病理学会関西部会
  • Place of Presentation: 和歌山
  • Year and Date: 20080918-20080919
• [Presentation] Molecular analysis of genes implicated in triterpene synthesis in rice. (2008)
  • Author(s): Inagaki Y, Hetherington G, Dicks J, Mutsukado Y, Toyoda K, Shiraishi T, Ichinose Y, Field B, Osbourn A
  • Organizer: 5th International Conference on Plant Metabolomics
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20080715-20080718
• [Presentation] サポニン生合成に関与するイネOxidosqualene cyclasse遺伝子の分子解析 (2008)
  • Author(s): 稲垣善茂・今岡敦子・藤田景子・荒瀬栄・豊田和弘・白石友紀・一瀬勇規・Anne Osbourn
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: 松江
  • Year and Date: 20080426-20080428
• [Presentation] イネにおけるサポニン生合成経路についての分子解析 (2008)
  • Author(s): 稲垣善茂, Anne Osbourn
  • Organizer: 第49回日本植物生理学会
  • Place of Presentation: 札幌
  • Year and Date: 20080320-20080322
• [Presentation] イネにおけるサポニン生合成経路遺伝子Oxidosqualene Cyclaseの分子解析 (2007)
  • Author(s): 稲垣善茂, Anne Osbourn
  • Organizer: 平成19年度日本植物病理学会関西部会
  • Place of Presentation: 岐阜
  • Year and Date: 20071006-20071007
• [Presentation] イネにおけるOxidosqualene Cyclase遺伝子群 (2007)
  • Author(s): 稲垣善茂, 睦門由果子, 豊田和弘, 白石友紀, 一瀬勇規, オズボーンアン
  • Organizer: のゲノム構造と遺伝子発現日本遺伝学会第79回大会
  • Place of Presentation: 岡山
  • Year and Date: 20070919-20070921
• [Presentation] Determination of Toxicity to Organisms in the Ashes from a Gasification Test Plant by Analyzing Transcriptional Change in a Model Plant Using a DNA Array Chip Loaded with 300 Oligonucleotides. (2007)
  • Author(s): Shiraishi M, Inagaki Y, Mohri S, Ono Y, Shiraishi T
  • Organizer: Society of Environmental Toxicology And Chemistry-Europe
  • Place of Presentation: Porto, Portugal
  • Year and Date: 20070521-20070524
• [Book] Pseudomonas syringaeのMAMPsと植物応答.ゲノム情報を活用した植物感染生理学の展望.(石井英夫, 他編) (2008)
  • Author(s): 一瀬勇規・内藤佳奈・薬師寺賢・常見和彦・Nguyen Linh Chi・田口富美子・鈴木智子・稲垣善茂・豊田和弘・白石友紀
  • Total Pages: 93-102
  • Publisher: 日本植物病理学会
• [Remarks]
  • URL: http://www.okayama-u.ac.jp/user/agr/nougaku0201.htm
• [Remarks]
  • URL: http://www.cc.okayama-u.ac.jp/~yinagaki/