An Overview image of the transcription network regulated by the CCAAT binding complex in Aspergillus oryzae

2008 Fiscal Year Final Research Report

• Project/Area Number: 19580088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Nagoya University
• Principal Investigator: KATO Masashi Nagoya University, 大学院・生命農学研究科, 准教授 (70242849)
• Project Period (FY): 2007 – 2008
• Keywords: 微生物 / ゲノム / マイクロアレイ / 発現制御 / 糸状菌 / 転写因子 / Hap複合体 / CCAAT-box

Research Abstract

本研究では麹菌Hap複合体により制御される遺伝子群を網羅的に同定し、それら遺伝子群の関連性を明らかにすることを目的とした。その結果、Hap複合体はリボソーマルタンパク質遺伝子などの翻訳関連遺伝子を間接的に抑制することが明らかとなった。また、HapXと協調して鉄に関連した酵素(チトクロームc、アコニターゼなど)や鉄の取り込み系の抑制因子SreAの遺伝子の発現を抑制することが明らかとなった。

Research Products

• [Journal Article] Identification of specific binding sites for XYR1, atranscriptional activator of cellulolytic and xylanolytic genes in Trichoderma reesei (2009)
  • Author(s): Furukawa T, Shida Y, Kitagami N, Mori K, Kato M, Kobayashi T, Okada H, Ogasawara W, Morikawa Y
  • Journal Title: Fungal Genet Biol (印刷中)
  • Peer Reviewed
• [Journal Article] Inducer-dependent nuclear localization of a Zn(II)(2)Cys(6)transcriptional activator, AmyR, in Aspergillus nidulans (2009)
  • Author(s): Makita T, Katsuyama Y, Tani S, Suzuki H, Kato N, Todd RB, Hynes MJ, Tsukagoshi N, Kato M, Kobayashi T
  • Journal Title: Biosci Biotechnol Biochem 73(2) Pages: 391-399
  • Peer Reviewed
• [Journal Article] Identification of the cis-acting elements involved in regulation of xylanase III gene expression in Trichoderma reesei PC-3-7 (2008)
  • Author(s): Furukawa T, Shida Y, Kitagami N, Ota Y, Adachi M, Nakagawa S, Shimada R, Kato M, Kobayashi T, Okada H, Ogasawara W, Morikawa Y
  • Journal Title: Fungal Genet Biol 45(7) Pages: 1094-1102
  • Peer Reviewed
• [Journal Article] analysis of the egl3 upstream region in filamentous fungus Trichoderma reesei (2008)
  • Author(s): Shida Y, Furukawa T, Ogasawara W, Kato M, Kobayashi T, Okada H, Morikawa Y. Functional
  • Journal Title: Appl Microbiol Biotechnol 78(3) Pages: 515-524
  • Peer Reviewed
• [Journal Article] GFP-tagged expression analysis revealed that some histidine kinases of Aspergillus nidulans show temporally and spatially different expression during the life cycle (2008)
  • Author(s): Suzuki A, Kanamaru K, Azuma N, Kato M, Kobayashi T
  • Journal Title: Biosci Biotechnol Biochem 72(2) Pages: 428-434
  • Peer Reviewed
• [Journal Article] Novel promoter sequence required for inductive expression of the Aspergillus nidulans endoglucanase gene eglA (2008)
  • Author(s): Endo Y, Yokoyama M, Morimoto M, Shirai K, Chikamatsu G, Kato N, Tsukagoshi N, Kato M, Kobayashi T
  • Journal Title: Biosci Biotechnol Biochem 72(2) Pages: 312-320
  • Peer Reviewed
• [Journal Article] In vitro analysis of His-Asp phosphorelays in Aspergillus nidulans: the first direct biochemicalevidence for the existence of His-Asp phosphotransfer systems in filamentous fungi (2007)
  • Author(s): Azuma N, Kanamaru K, Matsushika A, Yamashino T, Mizuno T, Kato M, Kobayashi T
  • Journal Title: Biosci Biotechnol Biochem 71(10) Pages: 2493-2502
  • Peer Reviewed
• [Journal Article] The SskA and SrrA response regulators are implicated in oxidative stress responses of hyphae and asexual spores in the phosphorelay signaling network of Aspergillus nidulans (2007)
  • Author(s): Hagiwara D, Asano Y, Marui J, Furukawa K, Kanamaru K, Kato M, Abe K, Kobayashi T, Yamashino T, Mizuno T
  • Journal Title: Biosci Biotechnol Biochem 71(4) Pages: 1003-14
  • Peer Reviewed
• [Journal Article] Genomics of Aspergillus oryzae (2007)
  • Author(s): Kobayashi T, Abe K, Asai K, Gomi K, Juvvadi PR, Kato M, Kitamoto K, Takeuchi M, Machida M.
  • Journal Title: Biosci Biotechnol Biochem 71(3) Pages: 646-670
  • Peer Reviewed
• [Journal Article] Characterization of the NikA histidine kinase implicated in the phosphorelay signal transduction of Aspergillus nidulans, with special reference to fungicide responses (2007)
  • Author(s): Hagiwara D, Matsubayashi Y, Marui J, Furukawa K, Yamashino T, Kanamaru K, Kato M, Abe K, Kobayashi T, Mizuno T
  • Journal Title: Biosci Biotechnol Biochem 71(3) Pages: 844-847
  • Peer Reviewed
• [Journal Article] Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron (2007)
  • Author(s): Hortschansky P, Eisendle M, Al-Abdallah Q, Schmidt AD, Bergmann S, Thon M, Kniemeyer O, Abt B, Seeber B, Werner ER, Kato M, Brakhage AA, Haas H
  • Journal Title: EMBO J 26(13) Pages: 3157-3168
  • Peer Reviewed
• [Presentation] 糸状菌CCAAT結合因子の存在量は栄養状態に応答する (2009)
  • Author(s): 伊奈慎太郎、高橋明珠、小林哲夫、加藤雅士
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] 糸状菌転写因子HapXによる転写抑制機構 (2009)
  • Author(s): 安藤栄里子、杉山純也、小林哲夫、加藤雅士
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] Interaction of HapX-HapB/C/Ecomplex with the cytochrome c promoter (2009)
  • Author(s): Ando E, Sugiyama J, Hortschansky P, Brakhage AA, Haas H, Kobayashi T, and Kato M
  • Organizer: 25th Fungal Genetics Conference at Asilomar
  • Place of Presentation: California, USA
  • Year and Date: 2009-03-19
• [Presentation] Analysis on the transcriptional regulation network by the CCAAT-binding complex as a key factor in Aspergillus spp. 2008 JSPS and KOSEF Joint Seminar. The Japan-Korea Joint Basic Scientific Cooperation Program (2008)
  • Author(s): Kato, M
  • Organizer: Pioneering Research on Fungal Molecular Biology
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2008-11-19
• [Presentation] Expression of ribosomal protein genes is depressed by the CCAAT-binding complex in Aspergillus species (2008)
  • Author(s): Takahashi A, Sugiyama J, Sano M, Kobayashi T, and Kato M
  • Organizer: 9th European Conference on Fungal Genetics
  • Place of Presentation: Edingurgh, Scotland
  • Year and Date: 2008-04-07
• [Presentation] Novel mechanisms of redox and iron regulation of fungal transcription factors (2008)
  • Author(s): Hortschansky P, Eisendle M, Al-Abdallah Q, Schmidt AD, Bergmann S, Thon M, Kniemeyer O, Abt B, Seeber BWerner ER, Kato M, Brakhage AA, Haas H
  • Organizer: 9th European Conference on Fungal Genetics
  • Place of Presentation: Edingurgh, Scotland
  • Year and Date: 2008-04-07
• [Presentation] 糸状菌CCAAT-box結合因子による転写制御ネットワーク : リボソーマルタンパク質の転写抑制機構 (2008)
  • Author(s): 高橋明珠、佐野元昭、小林哲夫、加藤雅士
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-27
• [Presentation] 麹菌CCAAT-box結合因子によるリボソーマルタンパク質遺伝子の発現抑制 (2007)
  • Author(s): 高橋明珠、杉山純也、佐野元昭、小林哲夫、加藤雅士
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京
  • Year and Date: 2007-03-25
• [Book] In "Aspergillus : Molecular Biology and Genomics".(eds by Machida M and Gomi K)(Transcriptional Regulantion in Aspergillus) (2009)
  • Author(s): Kobayashi T and Kato M
  • Total Pages: 85-114
  • Publisher: Caister Academic Press, Portland, USA
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~iden/japanese/index_j.html