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2008 Fiscal Year Final Research Report

Clarification of mechanism of biosynthesis and accumulation of a large amount of ascorbic acid

Research Project

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Project/Area Number 19580108
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionHiroshima University

Principal Investigator

ESAKA Muneharu  Hiroshima University, 大学院・生物圏科学研究科, 教授 (70151975)

Project Period (FY) 2007 – 2008
Keywordsアスコルビン酸 / アセロラ / 遺伝子発現 / ストレス抵抗性植物 / Cdna / ビタミンC / 酵素 / クローニング
Research Abstract

熱帯植物の一つであるアセロラは、特に果実に大量のアスコルビン酸を含む(レモンの20倍以上)。本研究は、植物のアスコルビン酸の増強を目的として、アセロラのアスコルビン酸生合成遺伝子をクローニングし、その発現機構を調べ、アセロラに大量のアスコルビン酸が生合成・集積する機構について明らかにするとともに、アセロラのアスコルビン酸生合成酵素遺伝子を植物に導入発現させ、アスコルビン酸高含量遺伝子組換え植物の作出をめざしたものですある。

  • Research Products

    (16 results)

All 2009 2008 2007

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (7 results)

  • [Journal Article] Increase in ascorbate content of transgenic tobacco plants overexpressing the acerola (Malpighia glabra) phosphomannomutase gene2009

    • Author(s)
      Badejo AA, Eltelib HA, Fukunaga K, Fujikawa Y, Esaka M.
    • Journal Title

      Plant Cell Physiol 50(2)

      Pages: 423-428

    • Peer Reviewed
  • [Journal Article] Gene expression of ascorbic acid biosynthesis related enzymes of Smirnoff-Wheeler pathway in acerola (Malpighia glabra). J.2009

    • Author(s)
      Badejo A.A, Fujikawa Y, Esaka M.
    • Journal Title

      Plant Physiol 166(6)

      Pages: 652-660

    • Peer Reviewed
  • [Journal Article] RNAi-mediated knockdown of the XIP-type endoxylanase inhibitor gene, OsXIP, has no effect on grain development and germination in rice2008

    • Author(s)
      Tokunaga T. Miyata Y. Fujikawa Y. Esaka M.
    • Journal Title

      Plant Cell Physiol 49(7)

      Pages: 1122-1127

    • Peer Reviewed
  • [Journal Article] Plant catalase is imported into peroxisomes by Pex5p but is distinct from typical PTS1 import2008

    • Author(s)
      Oshima Y. Kamigaki A. Nakamori C. Mano S. Hayashi M. Nishimura M. Esaka M.
    • Journal Title

      Plant Cell Physiol 49(4)

      Pages: 671-677

    • Peer Reviewed
  • [Journal Article] Analysis of GDP-D-mannose pyrophosphorylase gene promoter from acerola (Malpighia glabra) and increase in ascorbate content of transgenic tobacco expressing the acerola gene2008

    • Author(s)
      Badejo A.A, Tanaka N, Esaka M.
    • Journal Title

      Plant Cell Physiol 49(1)

      Pages: 126-132

    • Peer Reviewed
  • [Journal Article] Expression of multi-copy flavonoid pathway genes coincides with anthocyanin, flavonol and flavan-3-ol accumulation of grapevine2008

    • Author(s)
      Jeong ST. Goto-Yamamoto N. Hashizume K. Esaka M.
    • Journal Title

      Vitis 47

      Pages: 135-140

    • Peer Reviewed
  • [Journal Article] Induction of a novel XIP-type xylanase inhibitor by external ascorbic acid treatment and differential expression of XIP-family genes in rice2007

    • Author(s)
      Tokunaga T. Esaka M.
    • Journal Title

      Plant Cell Physiol 48

      Pages: 700-714

    • Peer Reviewed
  • [Journal Article] Cloning and expression of GDP-D-mannose pyrophosphorylase gene and ascorbic acid content of acerola (Malpighia glabra L.) fruit at ripening stages2007

    • Author(s)
      Badejo A.A. Jeong S.T. Goto-Yamamoto N. Esaka M.
    • Journal Title

      Plant Physiol. Biochem 45

      Pages: 665-672

    • Peer Reviewed
  • [Journal Article] Molecular cloning and expression of GDP-D-mannose 3", 5"-epimerase during fruit ripening in acerola2007

    • Author(s)
      Badejo A.A. Jeong S.T. Goto-Yamamoto N. Esaka M.
    • Journal Title

      Acta Horticulturae 763

      Pages: 91-97

    • Peer Reviewed
  • [Presentation] Engineering Increased Vitamin C Concentration in Transgenic Tobacco with Acerola2008

    • Author(s)
      Badejo AA, Eltelib HA, Fujikawa Y, Esaka M.
    • Organizer
      AHC 2008 (The Asian Horticultural Congress 2008)
    • Place of Presentation
      Seogwipo, Korea
    • Year and Date
      20081211-13
  • [Presentation] タバコのアスコルビン酸生合成におけるGPPaseの活性制御機構の解明2008

    • Author(s)
      坂本真吾、藤川愉吉、江坂宗春
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸市、神戸ポートアイランド
    • Year and Date
      2008-12-12
  • [Presentation] トマトにおけるアスコルビン酸生合成遺伝子ガラクツロン酸レダクターゼの遺伝子発現2008

    • Author(s)
      坂本真吾、村野亜沙子、藤川愉吉、江坂宗春
    • Organizer
      日本農芸化学会中四国支部第22回講演会
    • Place of Presentation
      鳥取市鳥取大学
    • Year and Date
      2008-09-13
  • [Presentation] イネのアスコルビン酸生合成と光応答2008

    • Author(s)
      福永一成、寺田龍司、加藤もも、江坂宗春
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋市名城大学
    • Year and Date
      2008-03-27
  • [Presentation] タバコにおけるアスコルビン酸生合成マンノース経路遺伝子の発現調節2008

    • Author(s)
      坂本真吾、江坂宗春
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋市名城大学
    • Year and Date
      2008-03-27
  • [Presentation] Analysis of the molecular property and expression profile of a novel xylanase inhibitor in rice2007

    • Author(s)
      Tokunaga T. Miyata Y. Esaka M.
    • Organizer
      The 5th International Symposium on the Molecular Breeding of Forage and Turf (MBFT)
    • Place of Presentation
      Sapporo, Hokkaido, Japan
    • Year and Date
      20070701-06
  • [Presentation] タバコにおけるアスコルビン酸生合成と生理機能の解明2007

    • Author(s)
      坂本真吾、福永一成、江坂宗春
    • Organizer
      日本農芸化学会中四国支部第18回講演会(例会)
    • Place of Presentation
      広島市、県立広島大学広島キャンパス
    • Year and Date
      2007-05-12

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Published: 2010-06-10   Modified: 2016-04-21  

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