2009 Fiscal Year Final Research Report
Characterization of a novel enzyme involved in the degradation of plant proteoglycans
| Project/Area Number |
19580112
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | National Agricultural Research Organization |
|---|
Principal Investigator |
KANEKO Satoshi National Agricultural Research Organization, 食品総合研究所食品バイオテクノロジー研究領域, 主任研究員 (90343821)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Keywords | 酵素利用学 / 糖質関連酵素 |
|---|
| Research Abstract |
A novel enzyme, beta-L-arabinopyranosidase, that liberates arabinose from thickening agent arabinogalactan was isolated from Streptomyces avermitilis, and the three dimentional structure was resolved. The enzyme belongs to the glycoside hydrolase family as same as alpha-D-galactosidases. One aspartic acid in the catalytic pocket of alpha-D-galactosidase changed to glutamic acid in that of beta-L-arabinopyranosidase, and the residue modulates the enzyme activity. Addifionally, it has an arabinopyranose-binding module.
|
