2009 Fiscal Year Final Research Report
Metabolism of n-3 polyunsaturated fatty acids by lipoxygenases and production of novel bioactive agents
| Project/Area Number |
19580138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Shimane University |
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Principal Investigator |
JISAKA Mitsuo Shimane University, 生物資源科学部, 准教授 (60243424)
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOTA Kazushige 島根大学, 生物資源科学部, 教授 (90158361)
NAGAYA Tsutomu 島根大学, 生物資源科学部, 准教授 (50284021)
NISHIMURA Kohji 島根大学, 総合科学研究支援センター, 助教 (30304257)
HUKUZAWA Kenji 安田女子大学, 薬学部, 教授
TAKAHASHI Yoshitaka 岡山県立大学, 保険福祉学部, 教授 (10236333)
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| Project Period (FY) |
2007 – 2009
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| Keywords | リポキシゲナーゼ / n-3系脂肪酸 / ドコサヘキサエン酸 |
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| Research Abstract |
Various bioactive agents are biosynthesized from docosahexaenoic acid (DHA). Although lipoxygenases (LOXs) are supposed to be involved in the biosynthetic pathways, details remain to be evaluated. In this research, we examined metabolism of DHA by LOX using purified enzymes and substrates. we focus on human 15-LOX-2, which may be involved in normal function of prostate tissue, and its mouse homologue, 8-LOX. 15-LOX-2 converted DHA solely to 17S-hydroperoxydocosahexaenoic acid (17S-HPDHA), while 8-LOX readily catalyzed double dioxygenation of DHA, producing 10S,17S-diHPDHA as a main product and 7S,17S-diHPDHA as a minor product. 10S,17S-diHPDHA, an isomer of neuroprotectin D1, is reported to suppress aggregation of human platelets. Our results may contribute effective production of some therapeutically significant DHA metabolites.
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[Journal Article] Tumor-Suppressive Lipoxygenase Inhibit the Expression of c-myc mRNA Coding Region Determinant-Binding Protein/Insulin-Like 5 Growth Factor II mRNA-Binding Protein 1 in Human Prostate Carcinoma PC-3 Cells.2009
Author(s)
Y. Kawakami, N. Kubota, N. Ekuni, T. Suzuki-Yamamoto, M. Kimoto, H. Yamashita, H. Tsuji, T. Yoshimoto, M. Jisaka, J. Tanaka, H.F. Fujimura, Y. Miwa, Y. Takahashi
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Journal Title
Bioscience, Biotechnology, and Biochemistry 73
Pages: 1811-1817
Peer Reviewed
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