Molecular mechanisms for dissolution of water-insoluble minerals containing phosphates by mycorrhizal fungi

2009 Fiscal Year Final Research Report

• Project/Area Number: 19580172
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 林学・森林工学
• Research Institution: Kyoto University
• Principal Investigator: HATTORI Takefumi Kyoto University, 生存圏研究所, 助教 (60212148)
• Co-Investigator(Kenkyū-buntansha): IWASE Koji 鳥取大学, 農学部, 教授 (60444642) ; UMEZAWA Toshiaki 京都大学, 生存圏研究所, 教授 (80151926)
• Co-Investigator(Renkei-kenkyūsha): YAMATO Masahide 鳥取大学, 農学部, 助教 (00571788)
• Project Period (FY): 2007 – 2009
• Keywords: 森林生物

Research Abstract

Ectomycorrhizal (ECM) fungi solubilize water-insoluble minerals containing phosphorus, making phosphate available in soluble form to be taken up by the host tree. Strong chelators, such as oxalate, citrate, and malate, released from ECM play a pivotal role in the dissolution of minerals through complexolysis. In this study, we attempted to clone a cDNA encoding a homologue of the oxalate-producing enzyme cytochrome c dependent glyoxylate dehydrogenase from Laccaria bicolor. We isolated cDNA encoding the cytochrome c dependent glyoxylate dehydrogenase (FpGLOXDH) from Fomitopsis palustris. We found that the homologue of FpGLOXDH (jgi|Lacbil|296363|er2 Lbscfoolog 01720, Laccaria bicolor) was present in the genome of L.bicolor. However, activity of FpGLOXDH was not detected in L.bicolor. Further research is needed to elucidate the mechanism underlying the biosynthesis of these organic acids.

Research Products

• [Journal Article] Subcellular localization of the oxalic acid-producing enzyme, cytochrome c dependent glyoxylate dehydrogenase, in brown-rot fungus Fomitopsis palustris (2007)
  • Author(s): Hattori, T., Okawa, K., Fujimura, M., Mizoguchi, M., Watanabe, T., Tokimatsu, T., Inui, H., Baba, K., Suzuki S., Umezawa, T., Shimada, M.
  • Journal Title: Cellulose Chemistry and Technology 51 Pages: 545-553
• [Presentation] アルミニウムによる外生菌根菌の有機酸代謝変動 (2010)
  • Author(s): 小篠貴臣, 大和麻子, 大和政秀, 岩瀬剛二, 鈴木史朗, 梅澤俊明, 服部武文
  • Organizer: 第60回日本木材学会大会
  • Place of Presentation: 宮崎(CD-ROM)
  • Year and Date: 20100317-20100319