2009 Fiscal Year Final Research Report
Development of transformation system in Lombardy poplar by using a poplar gene as a selective marker gene
| Project/Area Number |
19580178
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林学・森林工学
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
NISHIGUCHI Mitsuru Forestry and Forest Products Research Institute, 生物工学研究領域, 主任研究員 (80353796)
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| Project Period (FY) |
2007 – 2009
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| Keywords | ポプラ / 遺伝子組換え / 選抜マーカー遺伝子 / ツニカマイシン / GPT / バイナリーベクター / 遺伝子発現 / 出芽酵母 |
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| Research Abstract |
To obtain a selective marker gene for generation of transgenic trees, we cloned and sequenced the cDNA of UDP-N-acetylglucosamine : dolichyl-phosphate N-acetylglucosaminephosphotransferase (GPT) from Lombardy poplar (Populus nigra var. italica). Overexpression of GPT in budding yeast conferred tunicamycin tolerance. We succeeded in the generation of GPT-introduced transgenic poplars by using tunicamycin and a new binary vector, which contained the cDNA of GPT as a selective marker gene.
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