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2010 Fiscal Year Final Research Report

Transfer and expression of germ cells between New and Old World quails

Research Project

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Project/Area Number 19580308
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionShinshu University

Principal Investigator

ONO Tamao  Shinshu University, 農学部, 教授 (10177264)

Co-Investigator(Kenkyū-buntansha) ASANO Anshin  帝京大学, 医学部, 助教 (70459311)
Co-Investigator(Renkei-kenkyūsha) KAGAMI Hiroshi  信州大学, 農学部, 教授 (80308303)
SHIMADA Kiyoshi  ソウル大学(韓国), 農学生命科学研究科, 教授 (40065579)
TAGAMI Takahiro  独立行政法人農業・食品産業技術総合研究機構, 主任研究員 (60355104)
Project Period (FY) 2007 – 2010
Keywords家畜生産システム / 家禽繁殖 / ニホンウズラ / コリンウズラ / ニワトリ / ヒメウズラ / 新世界ウズラ / 旧世界ウズラ
Research Abstract

Basic biological information of the bobwhite quail belongs to New World quail was obtained. (1) Ex vivo culture system leading to hatching was devised. (2) PCR systems were put to practical use to identify its genome and sex. (3) New and Old world quails were different in chromosomal level. (4) Normal developmental tables were made. (5) Donor-derived DNA products were observed in chimeras between the bobwhite quail and Japanese quail embryos.

  • Research Products

    (36 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (21 results) (of which Peer Reviewed: 21 results) Presentation (12 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Viability and functionality of primordial germ cells after freeze-thaw in chickens2011

    • Author(s)
      Nakamura Y, Usui F, Ono T, Kagami H, TagamiT
    • Journal Title

      Journal of Poultry Science 48(1)

      Pages: 57-63

    • Peer Reviewed
  • [Journal Article] Novel method of gene transfer in birds : Intracytoplasmic sperm injection (ICSI) for green fluorescent protein (GFP) expression in quail blastoderms.2010

    • Author(s)
      Mizushima S, Mizushima S, Takagi S, Ono T, Shimada K.
    • Journal Title

      Biology of Reproduction 83(6)

      Pages: 965-969

    • Peer Reviewed
  • [Journal Article] Efficient system for preservation and regeneration of genetic resources in chicken : concurrent storage of primordial germ cells and live animals from early embryos of a rare indigenous fowl (Gifujidori).2010

    • Author(s)
      Nakamura Y, Usui F, Ono T, Kagami H, Tagami T.
    • Journal Title

      Reproduction, Fertility and Development 22(8)

      Pages: 1237-1246

    • Peer Reviewed
  • [Journal Article] Germl ine replacement by transfer of primordial germ cells into partially sterilized embryos in the chicken.2010

    • Author(s)
      Nakamura Y, Usui F, Ono T, Kagami H, Tagami T
    • Journal Title

      Biology of Reproduction 83(1)

      Pages: 130-137

    • Peer Reviewed
  • [Journal Article] A novel concentrating system of chicken stem cells by bone marrow side population cells.2010

    • Author(s)
      Usui F, Ono T, Kagami H
    • Journal Title

      Journal of Poultry Science 47(1)

      Pages: 53-56

    • Peer Reviewed
  • [Journal Article] Phospholipase Cz mRNA expression and its potency during spermatogenesi s for activation of quail oocyte as a sperm factor.2010

    • Author(s)
      Mizushima S, Ono T, Shimada K.
    • Journal Title

      Molecular Reproduction and Development 76(12)

      Pages: 1200-1207

    • Peer Reviewed
  • [Journal Article] Analysis of developmental changes in avian DNA methylation using a novel method for quantifying genome-wide DNA methylation.2009

    • Author(s)
      Usui F, Ono T, Kagami H.
    • Journal Title

      Journal of Poultry Science 46(4)

      Pages: 286-290

    • Peer Reviewed
  • [Journal Article] Novel system for degeneration of blood vessels by UV irradiation and subsequent regeneration using chick bone marrow cells.2009

    • Author(s)
      Usui F, Ono T, Kagami H
    • Journal Title

      Cells Tissues Organs 188(5)

      Pages: 348-355

    • Peer Reviewed
  • [Journal Article] Depletion of primordial germ cells (PGCs) by X-irradiation to extraembryonic region of chicken embryos and expression of xenotransplanted quail PGCs.2009

    • Author(s)
      Atsumi Y, Kagami H, Ono T
    • Journal Title

      journal of Poultry Science 46(2)

      Pages: 136-143

    • Peer Reviewed
  • [Journal Article] Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial germ cells in early chicken embryos.2009

    • Author(s)
      Nakamura Y, Ono T, Kagami H, Tagami T
    • Journal Title

      Journal of Poultry Science 46(2)

      Pages: 127-135

    • Peer Reviewed
  • [Journal Article] Complete regeneration of muscular dystrophy chickens by mating of male and female offspring derived from germline chimeras.2009

    • Author(s)
      Fujiwara A, Ono T Hiramatsu K, Kagami H
    • Journal Title

      Journal of Poultry Science 46(2)

      Pages: 123-126

    • Peer Reviewed
  • [Journal Article] Regeneration of muscular dystrophy chickens by transplantation of early blastodermal cells into recipient embryos.2009

    • Author(s)
      Fujiwara A, Ono T, Kagami H
    • Journal Title

      Journal of Poultry Science 46(1)

      Pages: 46-51

    • Peer Reviewed
  • [Journal Article] Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chickens.2008

    • Author(s)
      Nakamura Y, Ono T Kagami H, Tagami T
    • Journal Title

      Reproduction, Fertility and Development 20(8)

      Pages: 900-907

    • Peer Reviewed
  • [Journal Article] Restriction of germline proliferation by soft x-ray irradiation of chicken embryos and its application to chimera production.2008

    • Author(s)
      Atsumi Y, Tagami T, Kagami H, Ono T
    • Journal Title

      Journal of Poultry Science 45(4)

      Pages: 292-297

    • Peer Reviewed
  • [Journal Article] Developmental enhancement of intracytoplasmic sperm injection (ICSI)-generated quail embryos by phospholipase CζcRNA.2008

    • Author(s)
      Mizushima S, Ono T, Shimada K.
    • Journal Title

      Journal of Poultry Science 45(2)

      Pages: 152-158

    • Peer Reviewed
  • [Journal Article] Effects of aromatase inhibitor (fadrozole)-induced sex-reversal on gonadal differentiation and mRNA expression of P450arom, AMH and FRa in embryos and growth in posthatching quail.2008

    • Author(s)
      Koba N, Ono T, Shimada K.
    • Journal Title

      Journal of Poultry Science 45(2)

      Pages: 116-124

    • Peer Reviewed
  • [Journal Article] Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail (Coturnix japonica).2007

    • Author(s)
      Mizushima S, Ono T, Shimada K.
    • Journal Title

      journal of Experimental Zoology Part A : Ecological Genetics and Physiology 307A(11)

      Pages: 647-653

    • Peer Reviewed
  • [Journal Article] Migration and proliferation of primordial germ cells in the early chicken embryo.2007

    • Author(s)
      MNakamura Y, Ono T, Kagami H, Tagami T
    • Journal Title

      Poultry Science 86(10)

      Pages: 2182-2193

    • Peer Reviewed
  • [Journal Article] Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing a W chromosome.2007

    • Author(s)
      Takagi S, Ono T, Shimada K.
    • Journal Title

      Poultry Science 86(5)

      Pages: 937-943

    • Peer Reviewed
  • [Journal Article] A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken.2007

    • Author(s)
      Yamamoto Y, Ono T, Kagami H
    • Journal Title

      Biology of Reproduction 77(1)

      Pages: 115-119

    • Peer Reviewed
  • [Journal Article] Z-chromosome specific primers for chicken-quail hybrid blastoderm.2007

    • Author(s)
      Takagi S, Ono T, Shimada K.
    • Journal Title

      Journal of Poultry Science 44(2)

      Pages: 209-212

    • Peer Reviewed
  • [Presentation] Effect of X-ray irradiation on primordial germ cell-depletion in chicken embryos.2011

    • Author(s)
      Nakamura Y, Ono T, Kagami H, Tagami T
    • Organizer
      Fullpaper CD files of the 9th Asian Pacific Poultry Conference.Tue-S10-04,pp.1-4.
    • Place of Presentation
      Taipei, Republic of China
    • Year and Date
      2011-03-22
  • [Presentation] Down-regulation of inositol 1,4,5-trisphosphate receptor type 1 (TP_3R-1) for oocyte activation in quail.2011

    • Author(s)
      Mizushima S, Ono T, Shimada K.
    • Organizer
      Fullpaper CD files of the 9th Asian Pacific Poultry Conference.D-Tue-09, pp.1-4.
    • Place of Presentation
      Taipei, Republic of China
    • Year and Date
      2011-03-21
  • [Presentation] Down-regulation of inositol 1,4,5-trisphosphate receptor type 1 (IP_3R-1) for oocyte activation in quail.2011

    • Author(s)
      Shimada K, Mizushima S, Ono T, Kimura T
    • Organizer
      Fullpaper CD files of the 9th Asian Pacific Poultry Conference.D-Tue-09, pp.1-4.
    • Place of Presentation
      Taipei, Republic of China
    • Year and Date
      2011-03-21
  • [Presentation] Basic information on blue-breasted quail and northern bobwhite quail for germline transfer studies.2011

    • Author(s)
      Ono T, Kagami H, Tsudzuki M, Matsuda Y
    • Organizer
      2nd International Workshop on Preservation of Avian Primordial Germ Cells and Its Usage
    • Place of Presentation
      Naha, Okinawa
    • Year and Date
      2011-01-25
  • [Presentation] Depletion of primordial germ cells for the better xenotransplanted germline donor in avian embryos.2010

    • Author(s)
      Ono T.
    • Organizer
      GSR2010 Proceedings, WCU Biomodulation International Symposium on Germ Cell, tem Cell and Reproductive Biology
    • Place of Presentation
      Je ju, Korea
    • Year and Date
      2010-06-07
  • [Presentation] Intracytoplasmic sperm injection (ICSI) for avian transgenesis.2010

    • Author(s)
      Shimada K, Ono T, Mizushima S.
    • Organizer
      GSR2010 Proceedings, WCU Biomodulation International Symposium on Germ Cell, Stem Cell and Reproductive Biology
    • Place of Presentation
      Je ju, Korea
    • Year and Date
      2010-06-07
  • [Presentation] Establishment of intracytoplasmic sperm injection (ICSI) method for green fluorescent (GFP) expression in quail blastoderm.2010

    • Author(s)
      Mizushima S, Ono T, Shimada K.
    • Organizer
      Fullpaper CD files of the 9th Asian Pacific Poultry Conference.Mon-S4-06,pp.1-4.
    • Place of Presentation
      Taipei, Republic of China
    • Year and Date
      2010-03-22
  • [Presentation] cDNA cloning, mRNA expression and oocyte-activating potential of quail phospholipase Czata.2010

    • Author(s)
      Mizushima S, Ono T, Shimada K.
    • Organizer
      Proceedings of the 6th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology p. 80.
    • Place of Presentation
      Palmerston North, New Zealand
    • Year and Date
      2010-01-21
  • [Presentation] Roles of phospholipase C zeta, inositol trisphosphate and calcium in oocyte activation in quail.2010

    • Author(s)
      Shimada K Mizushima S, Ono T, Saito N.
    • Organizer
      Proceedings of the 6th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology p.63.
    • Place of Presentation
      Palmerston North, New Zealand
    • Year and Date
      2010-01-21
  • [Presentation] Germline replacement by transferring primordial germ cells into sterilized embryos in the chicken2009

    • Author(s)
      Nakamura Y, Ono T, Kagami H, Tagami T
    • Organizer
      2009 Annual Meeting Abstracts
    • Place of Presentation
      Poultry Science 88(suppl.1)
    • Year and Date
      2009-07-21
  • [Presentation] Restriction of proliferation of primordial germ cells by soft X-ray irradiation in chick embryos.2008

    • Author(s)
      Atsumi Y, Tagami T, Kagami H, Ono T
    • Organizer
      23rd World Poultry Congress
    • Place of Presentation
      Brisbane, Australia.
    • Year and Date
      2008-06-29
  • [Presentation] A novel strategy for preservation of genetic resources in birds.2008

    • Author(s)
      Nakamura Y, Ono T Kagami H, Tagami T
    • Organizer
      Abstracts, Society for the Study of Reproduction 41st Annual Meeting and World Congress on Reproductive Biology, p.203.
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2008-05-28
  • [Book] DNA多型VOL.17(ニワトリ初期胚におけるDNAメチル化状態の変化)(日本DNA多型学会編)2009

    • Author(s)
      臼井文武・小野珠乙・鏡味裕
    • Total Pages
      216-218
    • Publisher
      東洋書店
  • [Book] DNA多型VOL.15(DifferentialDisplayRT-PCRを用いたニワトリ初期胚での遺伝子発現様式)(日本DNA多型学会編)2007

    • Author(s)
      山本耕裕・福田靖・小野珠乙・鏡味靖
    • Total Pages
      74-76
    • Publisher
      東洋書店
  • [Remarks] ホームページ等

    • URL

      http://karamatsu.shinshu-u.ac.jp/lab/ono_kagami/hasseiken.htm

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Published: 2012-01-26   Modified: 2015-07-14  

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