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2008 Fiscal Year Final Research Report

Studies on development of avian embryo after ICSI

Research Project

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Project/Area Number 19580325
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionNagoya University

Principal Investigator

SHIMADA Kiyoshi  Nagoya University, 生命農学研究科, 名誉教授 (40065579)

Co-Investigator(Renkei-kenkyūsha) ONO Tamao  信州大学, 農学部, 教授 (10177264)
Project Period (FY) 2007 – 2008
Keywords細胞内精子顕微注入(ICSI) / 精子由来卵子活性化因子 / phospholipase PLCζ / cRNA / 胚盤葉 / 外来遺伝子GFP / トランスジェニックウズラ
Research Abstract

非ウィルス法による鳥類トランスジェニック作出は、成功率が極めて低い。よって本研究は、細胞質内精子顕微注射法(Intracytoplasmic Sperm Injection, ICSI)による遺伝子導入法を試みた。外来遺伝子としてGreen Fluorescent Protein(GFP)を単一射出精子とPLCzeta cRNAと同時に注入し、ウズラ胚にGFPを発現させるた。pCX-E GFPプラスミドとPLCzeta cRNAをウズラ未受精卵に同時に顕微注入し、24時間の体外培養(System Q1a)と48時間の追加体外培養の結果、外来遺伝子GFPを胚盤葉で発現していることが観察できた。

  • Research Products

    (13 results)

All 2009 2008 2007

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (3 results)

  • [Journal Article] Identification of differentially expressed genes involved in the regression and development of Mullerian duct of chicken2009

    • Author(s)
      Ha Y, Tsukada A, Saito N, Shimada K
    • Journal Title

      International Journal of Developmental Biology 52

      Pages: 1135-1141

    • Peer Reviewed
  • [Journal Article] Expressionof P450arom, AMH and Erα mRNA in gonads of turkey, duck and goose within one week of age2008

    • Author(s)
      Koba N, Ohfuji T, Ha Y, Mizushima S, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 45

      Pages: 215-219

    • Peer Reviewed
  • [Journal Article] Developmental enhancement of intracytoplasmic sperm injection(ICSI)-generated quail embryos by phospholipase Cζ2008

    • Author(s)
      Mizushima S, Takagi S, Ono T, Atsumi Y, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 45

      Pages: 152-158

    • Peer Reviewed
  • [Journal Article] Profiles of mRNA expression of FOXL2, P450arom, DMRT1, AMH. P450c17, SF1, Era and AR, in relation to gonadal sex differentiation in duck embryo2008

    • Author(s)
      Koba N, Ohfuji T, Ha Y, Mizushima S, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 45

      Pages: 132-138

    • Peer Reviewed
  • [Journal Article] Effects of aromatase inhibitor(Fadrozole)-induced sex-reversal on gonadal differentiation and mRNA expression of P450arom, AMH and Erα in quail embryos and growth in posthaching quail2008

    • Author(s)
      Koba N, Mori M, Ha Y, Mizushima S, Tsukada A, Saito N, Shimada K
    • Journal Title

      Journal of Poultry Science 45

      Pages: 116-124

    • Peer Reviewed
  • [Journal Article] Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail(Coturnix japonica)2008

    • Author(s)
      Mizushima S, Takagi S, Ono T, Atsumi Y, Tsukada A, Saito N, Shimada K
    • Journal Title

      Journal of Experimental Zoology 307

      Pages: 647-53

    • Peer Reviewed
  • [Journal Article] Histocompatible chicken inbred lines : Homogeneities in the major histocompatibility complex antigens of the GSP, GSN/1, PNP/DO and BM-C inbred lines assessed by hemagglutination, mixed lymphocyte reaction and skin transplantation2007

    • Author(s)
      Valdez Jr MB, Mizutani M, Fujihara A Yazawa H, Yamagata T, Shimada K, Namikawa T
    • Journal Title

      Experimental Animals 56

      Pages: 329-338

    • Peer Reviewed
  • [Journal Article] Z-chromosome specific primers for chicken-quail hybrid blastoderm2007

    • Author(s)
      Takagi S, Ono T, Tsukada A, Atsumi Y, Mizushima S, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 44

      Pages: 209-212

    • Peer Reviewed
  • [Journal Article] Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing the W chromosome2007

    • Author(s)
      Takagi S, Ono T, Tsukada A, Atsumi Y, Mizushima S, Saito N, Shimada K
    • Journal Title

      Poultry Science 86

      Pages: 937-943

    • Peer Reviewed
  • [Journal Article] Fertilizing ability of chicken sperm bearing W chromosome2007

    • Author(s)
      Takagi S, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Poultry Science 86

      Pages: 730-737

    • Peer Reviewed
  • [Presentation] ウズラ卵子DNase I活性に及ぼす精子数の効果2009

    • Author(s)
      杉本宰甫、水島秀成、古寺敏子、島田清司、小野珠乙、木村一郎
    • Organizer
      日本家禽学会
    • Place of Presentation
      藤沢市
    • Year and Date
      2009-03-28
  • [Presentation] 顕微授精ウズラ卵cRNA注入の効果2008

    • Author(s)
      水島秀成、島田清司
    • Organizer
      日本家禽学会
    • Place of Presentation
      水戸市
    • Year and Date
      2008-03-28
  • [Presentation] Profiles of mRNA expression of sex-differentiation-related genes in relation to gonadal sexual differentiation in duck2007

    • Author(s)
      Naomi Koba, Shusei Mizushima, Akira Tsukada, Noboru Saito
    • Organizer
      APPC
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2007-02-06

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Published: 2010-06-10   Modified: 2016-04-21  

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