2010 Fiscal Year Final Research Report
Temperature-dependent regulation of fowl sperm motility-as a model for signal transduction mechanisms of flagellar movement.
| Project/Area Number |
19580327
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | University of Miyazaki |
|---|
Principal Investigator |
ASHIZAWA Koji University of Miyazaki, 農学部, 教授 (60128353)
|
|---|
| Project Period (FY) |
2007 – 2010
|
| Keywords | 精子 / 鞭毛運動 / 運動調節 / 情報伝達 / リン酸化 |
|---|
| Research Abstract |
The possible role of PI3-K in the reversible temperature-dependent immobilization of fowl sperm motility was investigated by using PI3 kinase inhibitor. Intact fowl sperm motility in TES/NaCl buffer remained negligible at the avian body temperature of 40℃ but was activated immediately after the addition of Ca^<2+>. The subsequent addition of inhibitor inhibited the motility again. In contrast, these inhibition was not observed in the demembranated spermatozoa at 40℃. These results suggest that PI3 kinase which is not retained in the axoneme and/or accessory cytoskeletal components, but in the cell membrane or cytoplasm, may be involved in the maintenance of flagellar movement of fowl spermatozoa at 40℃.
|
-
-
[Journal Article] Temperature-dependent regulation of sperm motility of Ijima's copper pheasants (Syrmaticus soemmerringii ijimae), one of 'near threatened' species.2010
Author(s)
Ashizawa, K., Kawaji, N., Nakamura, S., Nagase, D., Tatemoto, H., Katayama, S., Narumi, K., Tsuzuki, Y.
-
Journal Title
Anim.Reprod.Sci. 121
Pages: 181-187
Peer Reviewed
-
[Journal Article] Effect of the addition of beta-hydroxybutyrate to chemically defined maturation medium on the nuclear maturation, sperm penetration and embryonic development of porcine oocytes in vitro.2010
Author(s)
Endo, R., Ishii, A., Nakanishi, A., Nabenishi, H., Ashizawa, K., Tsuzuki, Y.
-
Journal Title
Asian-Aust.J.Anim.Sci. 23
Pages: 1421-1426
Peer Reviewed
-
-
-
[Journal Article] Intracellular signal transduction pathways in the regulation of fowl sperm motility : Evidence for the involvement of phosphatidylinositol 3-kinase (PI3-K) cascade.2009
Author(s)
Ashizawa, K., Omura, Y., Katayama, S., Tatemoto, H., Narumi, K., Tsuzuki, Y.
-
Journal Title
Mol.Reprod.Develop. 76
Pages: 603-610
Peer Reviewed
-
-
[Journal Article] Characteristics of Okinawan native agu pig spermatozoa after addition of low-density lipoprotein to freezing extender.2009
Author(s)
Yamauchi, S., Nakamura, S., Lay, K.M., Azuma, T., Yakabi, T., Muto, N., Nakada, T., Ashizawa K., Tatemoto, H.
-
Journal Title
J.Reprod.Dev. 55
Pages: 558-565
Peer Reviewed
-
[Journal Article] Prediction of the estrous cycle and optimal insemination time by monitoring vaginal electrical resistance (VER) in order to improve the reproductive efficiency of the Okinawan native Agu pig.2009
Author(s)
Yamauchi, S., Nakamura, S., Yoshimoto, T., Hakada, T., Ashizawa, K., Tatemoto, H.
-
Journal Title
Anim.Reprod.Sci. 113
Pages: 311-316
Peer Reviewed
-
-
-
[Journal Article] Improvement of the post-thaw qualities of Okinawan native pig spermatozoa frozen in an extender supplemented with ascorbic acid 2-O-alpha-glucoside.2008
Author(s)
Yoshimoto, T., Nakamura, S., Yamauchi, S., Muto, N., Nakada, T., Ashizawa, K., Tatemoto H.
-
Journal Title
Cryobiology 57
Pages: 30-36
Peer Reviewed
-
[Journal Article] Antihyaluronidase action of ellagic acid effectively prevents polyspermy as a result of suppression of the acrosome reaction induced by sperm-zona interaction during in vitro fertilization of porcine oocytes.2007
Author(s)
Tokeshi, I., Yoshimoto, T., Muto, N., Nakamura, S., Ashizawa, K., Nakada, T., Tatemoto, H.
-
Journal Title
J.Reprod.Dev. 53
Pages: 755-764
Peer Reviewed
-
-
-
-
-
-
