Analysis of function and regulatory mechanism of mRNA expression of bird specific transcription factor in the anterior pituitary gland

2009 Fiscal Year Final Research Report

• Project/Area Number: 19580330
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Azabu University
• Principal Investigator: KANSAKU Norio Azabu University, 獣医学部, 教授 (60333142)
• Project Period (FY): 2007 – 2009
• Keywords: 生産機能制御 / 転写制御

Research Abstract

Intron 1 of the precocial and altricial bird of the Pit-1 gene were cloned and sequenced. Homologous position of putative translation starting site of Pit-1w/γ in the altricial species was not ATG methionin codon. Thus, it is suggested that Pit-1w/γ is not exist in the altricial species. Moreover, transcription starting region was cloned by RT-PCR. Results of this study showed that regulatory region of Pit-1w/γ is smaller than previously expected.

Research Products

• [Journal Article] Changes in post-translational modifications of prolactin during development and reproductive cycles in the chicken. (2009)
  • Author(s): Hiyama, G., Kansaku, N., Sasanami, T., Nakamura, A., Noda, K., Tsukada, A., Shimada, K., Zadworny, D.
  • Journal Title: General and Comparative Endocrinology. 161 Pages: 238-245
  • Peer Reviewed
• [Journal Article] Cloning of PRL and VIP cDNAs of the Java Sparrow (Padda oryzivora). (2009)
  • Author(s): Hiyama, G., Sato, T., Zadworny, D., Kansaku, N.
  • Journal Title: Animal Science Journal. 80 Pages: 176-186
  • Peer Reviewed
• [Journal Article] Cloning and Comparison of Prolactin Promoter in Galliformes. (2009)
  • Author(s): Hiyama, G., Zadworny, D., Kansaku, N.
  • Journal Title: Journal of Poultry Science. 46 Pages: 6-12
  • Peer Reviewed
• [Journal Article] Sequence of the domestic duck (Anas platyrhynchos) growth hormone-encoding gene and genetic variation in the promoter region. (2008)
  • Author(s): Kansaku, N., Soma, A., Furukawa, S., Hiyama, G., Okabayashi, H., Guemene, D., Kuhnlein, U., Zadworny, D.
  • Journal Title: Animal Science Journal. l79 Pages: 163-170
  • Peer Reviewed
• [Journal Article] Prolactin and growth hormone in bird: protein structure, gene structure, and genetic variation. (2008)
  • Author(s): Kansaku, N., Hiyama, G., Sasanami, T., Zadworny.
  • Journal Title: Journal of Poultry Science 45 Pages: 1-6
  • Peer Reviewed
• [Presentation] Detection of alternative splicing form of PRL mRNA in the chicken anterior pituitary gland. (2009)
  • Author(s): N. Kansaku, T. Sasanami, T. Ohkubo, G. Hiyama, D. Zadworny
  • Organizer: 2009 Joint Annual Meeting of the American Dairy Science Association, Canadian Society of Animal Science and the American Society of Animal Science
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2009-07-15
• [Presentation] Sequence comparison of the prolactin (PRL) promoter in BUT and Bettina turkeys (2008)
  • Author(s): Hiyama, G., Kansaku, N., Reddy, I.J., Sotocinal, S., Guemene, D., Zadworny, D.
  • Organizer: 9th International Symposium on Avian Endocrinology
  • Place of Presentation: Leuven Katholic University, Leuven, Belgium
  • Year and Date: 2008-07-13
• [Presentation] Comparison of Pit-1 intron-1 in birds. (2008)
  • Author(s): Kansaku, N., Tanaka, I., Tsushima, K., Zadworny, D.
  • Organizer: 9th International Symposium on Avian Endocrinology
  • Place of Presentation: Leuven Katholic University, Leuven, Belgium
  • Year and Date: 2008-07-12
• [Presentation] Comparison of prolactin in galliforms (2008)
  • Author(s): Hiyama G, Zadworny D, Ohtsuki M, Sasanami T, Kansaku N
  • Organizer: 9th International Symposium on Avian Endocrinology
  • Place of Presentation: Leuven Katholic University, Leuven, Belgium
  • Year and Date: 2008-07-11