2008 Fiscal Year Final Research Report
Effects of oxygen tension and follicle cells on maturation and fertilization of porcine oocytes during in vitro culture in follicular fluid
| Project/Area Number |
19580332
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
NAGAI Takashi National Agricultural Research Organization, 畜産草地研究所, 研究管理監 (20391378)
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| Project Period (FY) |
2007 – 2008
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| Keywords | ブタ / 体外成熟 / 体外受精 / 卵胞液 |
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| Research Abstract |
The objective was to investigate the effects of oxygen tension (5 or 20% O2) and follicle cells (FC) during in vitro maturation of porcine oocytes in only porcine follicular fluid, using static (S group) and non-static (rotating)(R group) culture systems, on their nuclear maturation and subsequent in vitro fertilization and development. Both -FC/S and +FC/R culture systems supported meiotic competence, irrespective of oxygen tension. However, the +FC/R culture system may be superior to the -FC/S culture system for promoting in vitro fertilization and development of in vitro matured porcine oocytes.
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